CRISPR-Cas-mediated targeted genome editing in human cells

Methods in Molecular Biology

Volumn 1114, Issue , 2014, Pages 245-267

  Yang, Luhan ^a   Mali, Prashant ^a   Kim Kiselak, Caroline ^a   Church, George ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Cas9; CRISPR; hiPSCs; Human genome engineering

Indexed keywords

CAS9 PROTEIN; CRISPR ASSOCIATED PROTEIN; ENDONUCLEASE; GUIDE RNA; RNA; UNCLASSIFIED DRUG;

ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; DNA CLEAVAGE; DNA END JOINING REPAIR; GENE DELIVERY SYSTEM; GENE SEQUENCE; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; HUMAN GENOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA SYNTHESIS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; STREPTOCOCCUS PYOGENES; AGAR GEL ELECTROPHORESIS; BACTERIUM CULTURE; CLONING VECTOR; EMBRYO; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSFECTION; KIDNEY CELL; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PROMOTER REGION; SPECTROPHOTOMETER; SYNTHESIS; TEMPERATURE; TIME; TISSUE CULTURE;

BACTERIA (MICROORGANISMS); STREPTOCOCCUS PYOGENES;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DNA END-JOINING REPAIR; GENE TARGETING; GENOME, HUMAN; HEK293 CELLS; HOMOLOGOUS RECOMBINATION; HUMANS; INDUCED PLURIPOTENT STEM CELLS; PLASMIDS; RNA EDITING; TRANSFECTION;

EID: 84922015344     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-761-7_16     Document Type: Article

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