Efficient simultaneous excision of multiple selectable marker cassettes using I-SceI-induced double-strand DNA breaks in Saccharomyces cerevisiae

FEMS Yeast Research

Volumn 14, Issue 5, 2014, Pages 741-754

  Solis Escalante, Daniel ^a   Kuijpers, Niels G A ^a   van der Linden, Franka H ^a   Pronk, Jack T ^a,b   Daran, Jean Marc ^a,b   Daran Lapujade, Pascale ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^b Platform Green Synthetic Biology   (Netherlands)

Author keywords

Auxotrophic marker; Budding yeast; Dominant marker; Double strand DNA break; I SceI; Marker excision

Indexed keywords

SACCHAROMYCES CEREVISIAE PROTEIN; SCEI PROTEIN, S CEREVISIAE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

DOUBLE STRANDED DNA BREAK; ENZYMOLOGY; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC SELECTION; GENETICS; HOMOLOGOUS RECOMBINATION; METABOLIC ENGINEERING; METABOLISM; MICROBIAL GENETICS; PLASMID; PROCEDURES; SACCHAROMYCES CEREVISIAE;

DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA BREAKS, DOUBLE-STRANDED; GENE DELETION; GENETICS, MICROBIAL; HOMOLOGOUS RECOMBINATION; METABOLIC ENGINEERING; MUTAGENESIS, INSERTIONAL; PLASMIDS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SELECTION, GENETIC;

EID: 84905577099     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/1567-1364.12162     Document Type: Article

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