Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides

BMC Immunology

Volumn 9, Issue , 2008, Pages

  Maecker, Holden T ^a   Hassler, Jeffrey ^b   Payne, Janice K ^c   Summers, Amanda ^d   Comatas, Karrie ^d   Ghanayem, Manar ^d   Morse, Michael A ^d   Clay, Timothy M ^d   Lyerly, Herbert K ^d   Bhatia, Sonny ^a   Ghanekar, Smita A ^a   Maino, Vernon C ^a   delaRosa, Corazon ^e   Disis, Mary L ^e  

^a BD BIOSCIENCES   (United States)

^b Fred Hutchinson Cancer Research Center   (United States)

^c BECKMAN COULTER INC   (United States)

^d DUKE CANCER INSTITUTE   (United States)

^e UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; CYTOMEGALOVIRUS ANTIGEN PP65; GAMMA INTERFERON; TETRAMER; PEPTIDE; VIRUS PROTEIN;

ACCURACY; ARTICLE; CD8+ T LYMPHOCYTE; CONTROLLED STUDY; CRYOPRESERVATION; DONOR; ENZYME LINKED IMMUNOSPOT ASSAY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; PERIPHERAL BLOOD MONONUCLEAR CELL; THAWING; VALIDATION PROCESS; CHEMISTRY; CYTOMEGALOVIRUS; ENZYME LINKED IMMUNOSORBENT ASSAY; METHODOLOGY; REPRODUCIBILITY;

CYTOMEGALOVIRUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMANS; INTERFERON TYPE II; PEPTIDES; REPRODUCIBILITY OF RESULTS; TISSUE DONORS; VIRAL PROTEINS;

EID: 41549126364     PISSN: None     EISSN: 14712172     Source Type: Journal    
DOI: 10.1186/1471-2172-9-9     Document Type: Article

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