Comparative characterization of the lactimidomycin and iso-migrastatin biosynthetic machineries revealing unusual features for acyltransferase-less type I polyketide synthases and providing an opportunity to engineer new analogues

Biochemistry

Volumn 53, Issue 49, 2014, Pages 7854-7865

  Seo, Jeong Woo ^a,d   Ma, Ming ^b   Kwong, Thomas ^b   Ju, Jianhua ^a,e   Lim, Si Kyu ^a,f   Jiang, Hui ^a,g   Lohman, Jeremy R ^b   Yang, Chunying ^b,h   Cleveland, John ^b   Zazopoulos, Emmanuel ^c   Farnet, Chris M ^c   Shen, Ben ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c Thallion Pharmaceuticals Inc   (Canada)

^d Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^e SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY   (China)

^f GenoTech Corporation   (South Korea)

^g ZHEJIANG UNIVERSITY   (China)

^h H LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOCHEMISTRY; CHEMICAL BONDS; CLONING; ENZYMES; GENES; KETONES; MACHINERY; PLANTS (BOTANY);

BIOSYNTHETIC GENE CLUSTER; CHEMICAL EQUATIONS; COMPARATIVE ANALYSIS; COMPARATIVE CHARACTERIZATIONS; DOUBLE SUBSTITUTION; POLYKETIDE SYNTHASES; STREPTOMYCES PLATENSIS; STRUCTURE ACTIVITY RELATIONSHIPS;

BIOSYNTHESIS;

8,9 DIHYDRO 8 HYDROXY LACTIMIDOMYCIN; 8,9 DIHYDRO 8 METHOXY LACTIMIDOMYCIN; 8,9 DIHYDRO 9 HYDROXY LACTIMIDOMYCIN; 8,9 DIHYDRO LACTIMIDOMYCIN; ACYLTRANSFERASE; ANTINEOPLASTIC AGENT; CYTOCHROME P450; GLUTARIMIDE DERIVATIVE; ISO MIGRASTATIN; LACTIMIDOMYCIN; POLYKETIDE SYNTHASE; UNCLASSIFIED DRUG; ANTINEOPLASTIC ANTIBIOTIC; BACTERIAL PROTEIN; ISOMIGRASTATIN; MACROLIDE; MUTANT PROTEIN; PIPERIDONE DERIVATIVE; POLYKETIDE; RECOMBINANT PROTEIN;

ANTIBIOTIC RESISTANCE; APOPTOSIS; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BREAST CANCER; CANCER CELL; CARBON NUCLEAR MAGNETIC RESONANCE; CONTROLLED STUDY; DNA SEQUENCE; DRUG SYNTHESIS; ESCHERICHIA COLI; FEMALE; GENE CLUSTER; GENE INACTIVATION; GENE OVEREXPRESSION; HELA CELL LINE; HETERONUCLEAR MULTIPLE BOND CORRELATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; JURKAT CELL LINE; MIGRATION; MOLECULAR CLONING; NONHUMAN; REGULATOR GENE; STREPTOMYCES; STREPTOMYCES AMPHIBIOSPORUS; STRUCTURE ACTIVITY RELATION; TUMOR CELL; UTERINE CERVIX CARCINOMA; ANTAGONISTS AND INHIBITORS; BIOLOGICAL MODEL; BIOREACTOR; BIOSYNTHESIS; CELL SURVIVAL; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; DRUG DESIGN; DRUG EFFECTS; ENZYMOLOGY; GENE SILENCING; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MULTIGENE FAMILY; NEOPLASMS; NUCLEOTIDE SEQUENCE; PROTEIN ENGINEERING; STEREOISOMERISM; TUMOR CELL LINE;

STREPTOMYCES AMPHIBIOSPORUS; STREPTOMYCES PLATENSIS;

ANTIBIOTICS, ANTINEOPLASTIC; BACTERIAL PROTEINS; BASE SEQUENCE; BIOREACTORS; CELL LINE, TUMOR; CELL SURVIVAL; DRUG DESIGN; GENE SILENCING; HUMANS; MACROLIDES; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MULTIGENE FAMILY; MUTANT PROTEINS; NEOPLASMS; PIPERIDONES; POLYKETIDE SYNTHASES; POLYKETIDES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; STEREOISOMERISM; STREPTOMYCES; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84918552662     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi501396v     Document Type: Article

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