Investigating the role of a backbone to substrate hydrogen bond in OMP decarboxylase using a site-specific amide to ester substitution

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 42, 2014, Pages 15066-15071

  Desai, Bijoy J ^a,b   Goto, Yuki ^c   Cembran, Alessandro ^d,g   Fedorov, Alexander A ^e   Almo, Steven C ^e   Gao, Jiali ^d,f   Suga, Hiroaki ^c   Gerlt, John A ^a,b  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c UNIVERSITY OF TOKYO   (Japan)

^d UNIVERSITY OF MINNESOTA   (United States)

^e ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^f JILIN UNIVERSITY   (China)

^g UNIVERSITY OF MINNESOTA DULUTH   (United States)

Author keywords

Cell free translation; Enzymology; Flexible tRNA acylation ribozyme; Unnatural protein residue

Indexed keywords

AMIDE; ESTER; LYSINE; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; OXYGEN; PYRIMIDINE; SERINE; RIBOZYME; TRANSFER RNA;

ARTICLE; CATALYSIS; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HYDROGEN BOND; METHANOBACTER THERMOAUTOTROPHICUM; METHANOBACTERIUM; MOLECULAR INTERACTION; MOLECULAR STABILITY; NONHUMAN; SUBSTITUTION REACTION; CELL FREE SYSTEM; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; EURYARCHAEOTA; MASS SPECTROMETRY; METABOLISM; MOLECULAR DYNAMICS; PH; PROTEIN SECONDARY STRUCTURE; PROTEIN SYNTHESIS; PROTEIN TERTIARY STRUCTURE; STATIC ELECTRICITY; TANDEM MASS SPECTROMETRY; TEMPERATURE; ULTRAVIOLET SPECTROPHOTOMETRY; X RAY CRYSTALLOGRAPHY;

AMIDES; CATALYSIS; CATALYTIC DOMAIN; CELL-FREE SYSTEM; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESTERS; EURYARCHAEOTA; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; PROTEIN BIOSYNTHESIS; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RNA, CATALYTIC; RNA, TRANSFER; SPECTROPHOTOMETRY, ULTRAVIOLET; STATIC ELECTRICITY; TANDEM MASS SPECTROMETRY; TEMPERATURE;

EID: 84908065770     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1411772111     Document Type: Article

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