The thiolase reaction mechanism: the importance of Asn316 and His348 for stabilizing the enolate intermediate of the Claisen condensation

Biochemistry

Volumn 48, Issue 46, 2009, Pages 11011-11025

  Meriläinen, Gitte ^a   Poikela, Visa ^a   Kursula, Petri ^a   Wierenga, Rik K ^a  

^a UNIVERSITY OF OULU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL-COA; ACTIVE SITE; C-C BOND FORMATION; CARBONYL CARBON; CATALYTIC BASE; CATALYTIC CAVITIES; CATALYTIC CYCLES; CATALYTIC MECHANISMS; CATALYTIC TRIAD; DUAL ROLE; ENOLATE INTERMEDIATES; ENOLATES; HYDROGEN BOND DONORS; HYDROGEN BONDING INTERACTIONS; MAIN CHAINS; NH GROUPS; OPTIMAL SETS; OXYANION HOLE; RATE-LIMITING STEPS; REACTION MECHANISM; STRUCTURAL CHANGE; TETRAHEDRAL INTERMEDIATES; TRANSITION STATE; TRANSITION STATE STABILIZATION; WATER MOLECULE;

AMINES; BINDING SITES; CATALYSIS; CONDENSATION; CONDENSATION REACTIONS; DEGRADATION; ENZYMES; FLUORINE CONTAINING POLYMERS; HYDROGEN; MOLECULES; POSITIVE IONS; QUANTUM CHEMISTRY; WATER ANALYSIS;

HYDROGEN BONDS;

ACETYL COENZYME A ACETYLTRANSFERASE;

ARTICLE; CATALYSIS; CHEMICAL BOND; CLAISEN CONDENSATION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME STRUCTURE; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL;

ACETYL COENZYME A; ACETYL-COA C-ACETYLTRANSFERASE; AMINO ACID SUBSTITUTION; ASPARAGINE; BIOCATALYSIS; CALORIMETRY; CATALYTIC DOMAIN; CIRCULAR DICHROISM; COENZYME A; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; HISTIDINE; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; STATIC ELECTRICITY; THERMODYNAMICS; TRANSITION TEMPERATURE; WATER; ZOOGLOEA;

EID: 72749112569     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi901069h     Document Type: Article

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