SCOPUS 정보 검색 플랫폼

Journal of the American Chemical Society

Volumn 129, Issue 43, 2007, Pages 12946-12947

Product deuterium isotope effect for orotidine 5′-monophosphate decarboxylase: Evidence for the existence of a short-lived carbanion intermediate

(6) Toth, Krisztina a Amyes, Tina L a Wood, Bryant M b Chan, Kui b Gerlt, John A b Richard, John P a

a UNIVERSITY AT BUFFALO (United States)

b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANION; CARBANION; DEUTERIUM; ISOTOPE; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL ANALYSIS; COMPLEX FORMATION; CRYSTAL STRUCTURE; DECARBOXYLATION; ENZYME BINDING; PHASE TRANSITION; PROTON TRANSPORT; REACTION ANALYSIS; STATISTICAL ANALYSIS; X RAY ANALYSIS; YEAST;

CARBON; DEUTERIUM; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR STRUCTURE; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; URIDINE MONOPHOSPHATE;

EID: 35848965479 PISSN: 00027863 EISSN: None Source Type: Journal
DOI: 10.1021/ja076222f Document Type: Article

Times cited : (44)

References (23)

1
- 35848948282
- Residues are numbered according to the sequence for the enzyme from yeast
- Residues are numbered according to the sequence for the enzyme from yeast.

2
- 0034524410
- Begley, T. P.; Appleby, T. C.; Ealick, S. E. Curr. Opin. Struct. Biol. 2000, 10, 711-718.
- (2000) Curr. Opin. Struct. Biol , vol.10 , pp. 711-718
- Begley, T.P.¹ Appleby, T.C.² Ealick, S.E.³

3
- 4644237751
- Begley, T. P.; Ealick, S. E. Curr. Opin. Chem. Biol. 2004, 8, 508-515.
- (2004) Curr. Opin. Chem. Biol , vol.8 , pp. 508-515
- Begley, T.P.¹ Ealick, S.E.²

4
- 0035997393
- Miller, B. G.; Wolfenden, R. Annu. Rev. Biochem. 2002, 71, 847-885.
- (2002) Annu. Rev. Biochem , vol.71 , pp. 847-885
- Miller, B.G.¹ Wolfenden, R.²

5
- 0028918401
- Radzicka, A.; Wolfenden, R. Science 1995, 267, 90-93.
- (1995) Science , vol.267 , pp. 90-93
- Radzicka, A.¹ Wolfenden, R.²

6
- 27844574242
- Amyes, T. L.; Richard, J. P.; Tait, J. J. J. Am. Chem. Soc. 2005, 127, 15708-15709.
- (2005) J. Am. Chem. Soc , vol.127 , pp. 15708-15709
- Amyes, T.L.¹ Richard, J.P.² Tait, J.J.³

7
- 0033612737
- Ehrlich, J. I.; Hwang, C.-C.; Cook, P. F.; Blanchard, J. S. J. Am. Chem. Soc. 1999, 121, 6966-6967.
- (1999) J. Am. Chem. Soc , vol.121 , pp. 6966-6967
- Ehrlich, J.I.¹ Hwang, C.-C.² Cook, P.F.³ Blanchard, J.S.⁴

8
- 0038998655
- Rishavy, M. A.; Cleland, W. W. Biochemistry 2000, 39, 4569-4574.
- (2000) Biochemistry , vol.39 , pp. 4569-4574
- Rishavy, M.A.¹ Cleland, W.W.²

9
- 0034102419
- (a) Wu, N.; Mo, Y.; Gao, J.; Pai, E. F. Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 2017-2022.
- (2000) Proc. Natl. Acad. Sci. U.S.A , vol.97 , pp. 2017-2022
- Wu, N.¹ Mo, Y.² Gao, J.³ Pai, E.F.⁴

10
- 24644514643
- (b) Gao, J.; Byun, K. L.; Kluger, R. Top. Curr. Chem. 2004, 238, 113-136.
- (2004) Top. Curr. Chem , vol.238 , pp. 113-136
- Gao, J.¹ Byun, K.L.² Kluger, R.³

11
- 0034610398
- (c) Warshel, A.; Strajbl, M.; Villa, J.; Florian, J. Biochemistry 2000, 39, 14728-14738.
- (2000) Biochemistry , vol.39 , pp. 14728-14738
- Warshel, A.¹ Strajbl, M.² Villa, J.³ Florian, J.⁴

12
- 34548250959
- Tsang, W.-Y.; Richard, J. P. J. Am. Chem. Soc. 2007, 129, 10330-10331.
- (2007) J. Am. Chem. Soc , vol.129 , pp. 10330-10331
- Tsang, W.-Y.¹ Richard, J.P.²

13
- 35848964267
- 1) and with suppression of the water peak. Baselines were subjected to first-order drift correction before integration of the signals.
- 1) and with suppression of the water peak. Baselines were subjected to first-order drift correction before integration of the signals.

14
- 0001735348
- Reaction mixtures were buffered with 50 mM 3-(N-morpholino) propanesulfonic acid (50% free base). Values of pL were obtained by adding 0.18 to the reading of the pH meter [Pentz, L.; Thornton, E. R. J. Am. Chem. Soc. 1967, 89, 6931-6938].
- Reaction mixtures were buffered with 50 mM 3-(N-morpholino) propanesulfonic acid (50% free base). Values of pL were obtained by adding 0.18 to the reading of the pH meter [Pentz, L.; Thornton, E. R. J. Am. Chem. Soc. 1967, 89, 6931-6938].

15
- 35848969958
- D (eq 1) remains constant over a period of ca. 20 hours. The variation in this ratio determined from integration of different NMR spectra is less than 3%.
- D (eq 1) remains constant over a period of ca. 20 hours. The variation in this ratio determined from integration of different NMR spectra is less than 3%.

16
- 0036040211
- Harris, P.; Poulsen, J.-C. N.; Jensen, K. F.; Larsen, S. J. Mol. Biol. 2002, 318, 1019-1029.
- (2002) J. Mol. Biol , vol.318 , pp. 1019-1029
- Harris, P.¹ Poulsen, J.-C.N.² Jensen, K.F.³ Larsen, S.⁴

17
- 0034104285
- Miller, B. G.; Hassell, A. M.; Wolfenden, R.; Milburn, M. V.; Short, S. A. Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 2011-2016.
- (2000) Proc. Natl. Acad. Sci. U.S.A , vol.97 , pp. 2011-2016
- Miller, B.G.¹ Hassell, A.M.² Wolfenden, R.³ Milburn, M.V.⁴ Short, S.A.⁵

18
- 0020346954
- Schowen, K. B.; Schowen, R. L. Methods Enzymol. 1982, 87, 551-606.
- (1982) Methods Enzymol , vol.87 , pp. 551-606
- Schowen, K.B.¹ Schowen, R.L.²

19
- 0000397708
- (a) Thibblin, A.; Jencks, W. P. J. Am. Chem. Soc. 1979, 101, 4963-4973.
- (1979) J. Am. Chem. Soc , vol.101 , pp. 4963-4973
- Thibblin, A.¹ Jencks, W.P.²

20
- 33847086871
- (b) Jencks, W. P. Acc. Chem. Res. 1980, 13, 161-169.
- (1980) Acc. Chem. Res , vol.13 , pp. 161-169
- Jencks, W.P.¹

21
- 0001762590
- Fishbein, J. C.; Jencks, W. P. J. Am. Chem. Soc. 1988, 110, 5075-5086.
- (1988) J. Am. Chem. Soc , vol.110 , pp. 5075-5086
- Fishbein, J.C.¹ Jencks, W.P.²

22
- 35848962176
- The primary 13C KIE on OMPDC-catalyzed decarboxylation of OMP labeled at the carboxylate carbon decreases from 1.043 for reaction in H2O to 1.034 for the 25% slower reaction in D2O ref 7, which shows that decarboxylation is less rate-determining in D 2O than in H2O. This result is not easily rationalized by a mechanism in which proton transfer from Lys-93 to C-6 of OMP is concerted with the loss of CO2 because the change from H2O to D2O should raise the barrier to a reaction in which proton transfer is concerted with loss of CO2, as a result of a normal primary KIE. This would cause the loss of CO2 to become more rate-determining in a multistep enzymatic reaction in D2O and would result in an increase, rather than the observed decrease, in the 13C isotope effect
- 13C isotope effect.

23
- 0034716756
- Richard, J. P.; Tsuji, Y. J. Am. Chem. Soc. 2000, 122, 3963-3964.
- (2000) J. Am. Chem. Soc , vol.122 , pp. 3963-3964
- Richard, J.P.¹ Tsuji, Y.²

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.