Product deuterium isotope effects for orotidine 5′-monophosphate decarboxylase: Effect of changing substrate and enzyme structure on the partitioning of the vinyl carbanion reaction intermediate

Journal of the American Chemical Society

Volumn 132, Issue 20, 2010, Pages 7018-7024

  Toth, Krisztina ^a,b   Amyes, Tina L ^a,b   Wood, Bryant M ^a,b   Chan, Kui ^a,b   Gerlt, John A ^a,b   Richard, John P ^a,b  

^a UNIVERSITY AT BUFFALO   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOND ROTATIONS; CONCERTED REACTIONS; DECARBOXYLASES; DEUTERIUM ISOTOPE EFFECT; ENZYME STRUCTURES; METHANOTHERMOBACTER THERMAUTOTROPHICUS; MONOPHOSPHATES; PRODUCT YIELDS; REACTION INTERMEDIATE; S.CEREVISIAE; SACCHAROMYCES CEREVISIAE; SIDE CHAINS;

CARRIER MOBILITY; DEUTERIUM; ENZYMES; ESCHERICHIA COLI; REACTION INTERMEDIATES;

CARBOXYLATION;

5 FLUOROOROTIDINE 5' MONOPHOSPHATE; CARBON DIOXIDE; CARBOXYLYASE; DEUTERIUM; LYSINE; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; UNCLASSIFIED DRUG; URIDINE PHOSPHATE; VINYL DERIVATIVE;

ARTICLE; BACTERIUM; CHEMICAL BOND; DECARBOXYLATION; ENZYME ACTIVE SITE; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; METHANOTHERMOBACTER THERMAUTOTROPHICUS; MUTATION; NONHUMAN; PROTON TRANSPORT; SACCHAROMYCES CEREVISIAE;

BIOCATALYSIS; DEUTERIUM; ESCHERICHIA COLI; METHANOBACTERIACEAE; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; RIBONUCLEOTIDES; SACCHAROMYCES CEREVISIAE; URIDINE MONOPHOSPHATE;

EID: 77952556805     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja102408k     Document Type: Article

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