Cell-free translation reconstituted with purified components

Nature Biotechnology

Volumn 19, Issue 8, 2001, Pages 751-755

  Shimizu, Yoshihiro ^a   Inoue, Akio ^a   Tomari, Yukihide ^a   Suzuki, Tsutomu ^a   Yokogawa, Takashi ^b   Nishikawa, Kazuya ^b   Ueda, Takuya ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b GIFU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CHROMATOGRAPHY; PROTEINS; RNA;

CELL-FREE TRANSLATION;

BIOTECHNOLOGY;

DIHYDROFOLATE REDUCTASE; TRANSFER RNA;

AFFINITY CHROMATOGRAPHY; AMINO ACID SUBSTITUTION; APPARATUS; ARTICLE; ENZYME ACTIVITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

AMINO ACIDS; CELL-FREE SYSTEM; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HISTIDINE; PLASMIDS; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; RIBOSOMES; RNA, TRANSFER; RNA, TRANSFER, AMINO ACYL; TIME FACTORS;

EID: 0034904102     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/90802     Document Type: Article

References (26)

1. The potentials of the in vitro protein biosynthesis system
2. Recent advances in producing and selecting functional proteins by using cell-free translation
3. Cooperativity of stabilized mRNA and enhanced translation activity in the cell-free system
4. Effect of the ATP level on the overall protein biosynthesis rate in a wheat germ cell-free system
5. A continuous cell-free translation system capable of producing polypeptides in high yield
6. A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane
7. DNA-directed in vitro synthesis of beta-galactosidase. Studies with purified factors
8. Isolation and point of action of a factor from Escherichia coli required to reconstruct translation
9. Rate of translation of natural mRNAs in an optimized in vitro system
10. Release factor RF3 abolishes competition between release factor RF1 and ribosome recycling factor (RRF) for a ribosome binding site
11. Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli
12. The mechanism of action of inititation factor F1 from Eschenchia coli
13. The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers
14. A general method for site-specific incorporation of unnatural amino acids into proteins
15. A highly efficient cell-free protein synthesis system from Escherichia coli
16. Identification of a soluble protein that stimulates peptide bond synthesis
17. Purification and properties of peptidyl-tRNA hydrolase from Escherichia coli
18. Molecular characterization of a prokaryotic translation factor homologous to the eukaryotic initiation factor elF4A
19. Isolation and analysis of ribosomes from prokaryotes, eukaryotes, and organelles
20. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli
21. Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro
22. Nucleoside triphosphate regeneration decreases the frequency of translation errors
23. Rate of elongation of polyphenylalanine in vitro
24. Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli
25. L-arabinose operon messenger of Escherichia coli. Its inducibility and translation efficiency relative to lactose operon messenger
26. Effect of release factor 1 on in vitro protein translation and the elaboration of protein containing unnatural amino acids