Characterization of the covalent binding of allyl isothiocyanate to β -lactoglobulin by fluorescence quenching, equilibrium measurement, and mass spectrometry

Journal of Biomolecular Structure and Dynamics

Volumn 32, Issue 7, 2014, Pages 1103-1117

  Keppler, Julia Katharina ^a   Koudelka, Tomas ^a   Palani, Kalpana ^a   Stuhldreier, Mayra Christina ^a   Temps, Friedrich ^a   Tholey, Andreas ^a   Schwarz, Karin ^a  

^a UNIVERSITY OF KIEL   (Germany)

Author keywords

allyl isothiocyanate; covalent ligand binding; fluorescence quenching; tryptic digestion; lactoglobulin

Indexed keywords

ALLYL ISOTHIOCYANATE; AMINE; BETA LACTOGLOBULIN; CYSTEINE; THIOL GROUP; ISOTHIOCYANIC ACID DERIVATIVE; LACTOGLOBULIN; LIGAND; PROTEIN BINDING;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; COMPLEX FORMATION; CONTROLLED STUDY; COVALENT BOND; DISSOCIATION CONSTANT; DISULFIDE BOND; ELECTROSPRAY MASS SPECTROMETRY; EQUILIBRIUM DIALYSIS; FLUORESCENCE ANALYSIS; FLUORESCENCE QUENCHING; HEADSPACE WATER EQUILIBRIUM; INTERMETHOD COMPARISON; LIGAND BINDING; MASS SPECTROMETRY; MEASUREMENT; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DENATURATION; PROTEIN INTERACTION; PROTEIN LIGAND BINDING; PROTEIN UNFOLDING; REPRODUCIBILITY; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; TANDEM MASS SPECTROMETRY; CHEMISTRY; SPECTROFLUOROMETRY;

ISOTHIOCYANATES; LACTOGLOBULINS; LIGANDS; MASS SPECTROMETRY; PROTEIN BINDING; SPECTROMETRY, FLUORESCENCE;

EID: 84899946156     PISSN: 07391102     EISSN: 15380254     Source Type: Journal    
DOI: 10.1080/07391102.2013.809605     Document Type: Article

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