Probenecid blocks human P2X7 receptor-induced dye uptake via a pannexin-1 independent mechanism

PLoS ONE

Volumn 9, Issue 3, 2014, Pages

  Bhaskaracharya, Archana ^a   Dao Ung, Phuong ^a   Jalilian, Iman ^b   Spildrejorde, Mari ^b   Skarratt, Kristen K ^a   Fuller, Stephen J ^a   Sluyter, Ronald ^b   Stokes, Leanne ^a,c  

^a UNIVERSITY OF SYDNEY   (Australia)

^b UNIVERSITY OF WOLLONGONG   (Australia)

^c RMIT UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CARBENOXOLONE; CARRIER PROTEIN; DYE; INTERLEUKIN 1BETA; PANNEXIN 1; PROBENECID; PURINERGIC P2X7 RECEPTOR; UNCLASSIFIED DRUG; ETHIDIUM; FLUORESCENT DYE; GAP JUNCTION PROTEIN; ISOQUINOLINE DERIVATIVE; LIPOPOLYSACCHARIDE; LUCIFER YELLOW; NERVE PROTEIN; PANX1 PROTEIN, HUMAN; PURINERGIC P2X RECEPTOR ANTAGONIST;

ARTICLE; CALCIUM TRANSPORT; CONTROLLED STUDY; CYTOKINE RELEASE; HEK293 CELL LINE; HUMAN; HUMAN CELL; IC 50; MONOCYTE; PATCH CLAMP TECHNIQUE; ACTIVE TRANSPORT; CALCIUM SIGNALING; IC50; IMMUNOLOGY; METABOLISM; PHYSIOLOGY; SECRETION (PROCESS);

ADENOSINE TRIPHOSPHATE; BIOLOGICAL TRANSPORT, ACTIVE; CALCIUM SIGNALING; CONNEXINS; ETHIDIUM; FLUORESCENT DYES; HEK293 CELLS; HUMANS; INHIBITORY CONCENTRATION 50; INTERLEUKIN-1BETA; ISOQUINOLINES; LIPOPOLYSACCHARIDES; MONOCYTES; NERVE TISSUE PROTEINS; PROBENECID; PURINERGIC P2X RECEPTOR ANTAGONISTS; RECEPTORS, PURINERGIC P2X7;

EID: 84899801491     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0093058     Document Type: Article

References (37)

1. Surprenant A, Rassendren F, Kawashima E, North RA, Buell G (1996) The cytolytic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 272: 735-738.
2. Wiley JS, Sluyter R, Gu BJ, Stokes L, Fuller SJ (2011) The human P2X7 receptor and its role in innate immunity. Tissue Antigens 78: 321-332.
3. Schachter J, Motta AP, de Souza Zamorano A, da Silva-Souza HA, Guimaraes MZ, et al. (2008) ATP-induced P2X7-associated uptake of large molecules involves distinct mechanisms for cations and anions in macrophages. J Cell Sci 121: 3261-3270.
4. Ferrari D, Pizzirani C, Adinolfi E, Lemoli RM, Curti A, et al. (2006) The P2X7 receptor: a key player in IL-1 processing and release. Journal of Immunology 176: 3877-3883. (Pubitemid 44264361)
5. Sluyter R, Dalitz JG, Wiley JS (2004) P2X7 receptor polymorphism impairs extracellular adenosine 5′-triphosphate-induced interleukin-18 release from human monocytes. Genes Immun 5: 588-591. (Pubitemid 39529976)
6. Sluyter R, Shemon AN, Wiley JS (2004) Glu496 to Ala polymorphism in the P2X7 receptor impairs ATP-induced IL-1 beta release from human monocytes. J Immunol 172: 3399-3405. (Pubitemid 38337918)
7. Pelegrin P, Surprenant A (2006) Pannexin-1 mediates large pore formation and interleukin-1beta release by the ATP-gated P2X7 receptor. EMBOJ 25: 5071-5082. (Pubitemid 44658525)
8. Qu Y, Misaghi S, Newton K, Gilmour LL, Louie S, et al. (2011) Pannexin-1 is required for ATP release during apoptosis but not for inflammasome activation. J Immunol 186: 6553-6561.
9. Hanley PJ, Kronlage M, Kirschning C, del Rey A, Di Virgilio F, et al. (2012) Transient P2X7 receptor activation triggers macrophage death independent of Toll-like receptors 2 and 4, caspase-1, and pannexin-1 proteins. J Biol Chem 287: 10650-10663.
10. Pelegrin P, Surprenant A (2007) Pannexin-1 couples to maitotoxin- and nigericin-induced interleukin-1beta release through a dye uptake-independent pathway. J Biol Chem 282: 2386-2394. (Pubitemid 47076789)
11. Kanneganti TD, Lamkanfi M, Kim YG, Chen G, Park JH, et al. (2007) Pannexin-1-mediated recognition of bacterial molecules activates the cryopyrin inflammasome independent of Toll-like receptor signaling. Immunity 26: 433-443. (Pubitemid 46603000)
12. Alberto AVP, Faria RX, Couto CGC, Ferreira LGB, Souza CAM, et al. (2013) Is pannexin the pore associated with the P2X7 receptor? Naunyn-Schmiedeberg's Archives of Pharmacology 386: 775-787.
13. Stokes L, Fuller SJ, Sluyter R, Skarratt KK, Gu BJ, et al. (2010) Two haplotypes of the P2X(7) receptor containing the Ala-348 to Thr polymorphism exhibit a gain-of-function effect and enhanced interleukin-1beta secretion. The FASEB Journal 24: 2916-2927.
14. Steinberg TH, Newman AS, Swanson JA, Silverstein SC (1987) Macrophages possess probenecid-inhibitable organic anion transporters that remove fluorescent dyes from the cytoplasmic matrix. J Cell Biol 105: 2695-2702. (Pubitemid 18017700)
15. Ma W, Hui H, Pelegrin P, Surprenant A (2009) Pharmacological characterization of pannexin-1 currents expressed in mammalian cells. J Pharmacol Exp Ther 328: 409-418.
16. Silverman W, Locovei S, Dahl G (2008) Probenecid, a gout remedy, inhibits pannexin 1 channels. Am J Physiol Cell Physiol 295: C761-767.
17. Bartlett R, Yerbury JJ, Sluyter R (2013) P2X7 Receptor Activation Induces Reactive Oxygen Species Formation and Cell Death in Murine EOC13 Microglia. Mediators of Inflammation 2013: 271813.
18. Di Virgilio F, Steinberg TH, Swanson JA, Silverstein SC (1988) Fura-2 secretion and sequestration in macrophages. A blocker of organic anion transport reveals that these processes occur via a membrane transport system for organic anions. J Immunol 140: 915-920. (Pubitemid 18067283)
19. Deeley RG, Westlake C, Cole SP (2006) Transmembrane transport of endo- and xenobiotics by mammalian ATP-binding cassette multidrug resistance proteins. Physiol Rev 86: 849-899. (Pubitemid 44033384)
20. Greene TA, Alarcon S, Thomas A, Berdougo E, Doranz BJ, et al. (2011) Probenecid inhibits the human bitter taste receptor TAS2R16 and suppresses bitter perception of salicin. PLoS One 6: e20123.
21. McClenaghan C, Zeng F, Verkuyl JM (2012) TRPA1 Agonist Activity of Probenecid Desensitizes Channel Responses: Consequences for Screening. Assay Drug Dev Technol 10: 533-541.
22. Bang S, Kim KY, Yoo S, Lee SH, Hwang SW (2007) Transient receptor potential V2 expressed in sensory neurons is activated by probenecid. Neurosci Lett 425: 120-125. (Pubitemid 47410367)
23. Gadeock S, Pupovac A, Sluyter V, Spildrejorde M, Sluyter R (2012) P2X7 receptor activation mediates organic cation uptake into human myeloid leukaemic KG-1 cells. Purinergic Signal 8: 669-676.
24. Roch-Ramel F, Guisan B, Diezi J (1997) Effects of Uricosuric and Antiuricosuric Agents on Urate Transport in Human Brush-Border Membrane Vesicles. Journal of Pharmacology and Experimental Therapeutics 280: 839-845. (Pubitemid 27171347)
25. Shemon AN, Sluyter R, Conigrave AD, Wiley JS (2004) Chelerythrine and other benzophenanthridine alkaloids block the human P2X7 receptor. Br J Pharmacol 142: 1015-1019. (Pubitemid 39037051)
26. Shemon AN, Sluyter R, Stokes L, Manley PW, Wiley JS (2008) Inhibition of the human P2X7 receptor by a novel protein tyrosine kinase antagonist. Biochem Biophys Res Commun 365: 515-520. (Pubitemid 350181690)
27. Michel AD, Thompson KM, Simon J, Boyfield I, Fonfria E, et al. (2006) Species and response dependent differences in the effects of MAPK inhibitors on P2X(7) receptor function. Br J Pharmacol 149: 948-957. (Pubitemid 44851719)
28. Pupovac A, Stokes L, Sluyter R (2013) CAY10593 inhibits the human P2X7 receptor independently of phospholipase D1 stimulation. Purinergic Signalling: 9: 609-619.
29. Takahashi A, Camacho P, Lechleiter JD, Herman B (1999) Measurement of intracellular calcium. Physiol Rev 79: 1089-1125. (Pubitemid 29473312)
30. Khanna D, Fitzgerald JD, Khanna PP, Bae S, Singh MK, et al. (2012) 2012 American College of Rheumatology guidelines for management of gout. Part 1: systematic nonpharmacologic and pharmacologic therapeutic approaches to hyperuricemia. Arthritis Care Res 64: 1431-1446.
31. Martinon F (2010) Mechanisms of uric acid crystal-mediated autoinflammation. Immunol Rev 233: 218-232.
32. Martinon F, Petrilli V, Mayor A, Tardivel A, Tschopp J (2006) Gout-associated uric acid crystals activate the NALP3 inflammasome. Nature 440: 237-241. (Pubitemid 43372104)
33. Riteau N, Baron L, Villeret B, Guillou N, Savigny F, et al. (2012) ATP release and purinergic signaling: a common pathway for particle-mediated inflammasome activation. Cell Death Dis 3: e403.
34. Kingsbury SR, Conaghan PG, McDermott MF (2011) The role of the NLRP3 inflammasome in gout. J Inflamm Res 4: 39-49.
35. Piccini A, Carta S, Tassi S, Lasiglie D, Fossati G, et al. (2008) ATP is released by monocytes stimulated with pathogen-sensing receptor ligands and induces IL-1beta and IL-18 secretion in an autocrine way. Proc Natl Acad SciUS A 105: 8067-8072. (Pubitemid 351904709)
36. Marques-da-Silva C, Chaves MM, Castro NG, Coutinho-Silva R, Guimaraes MZ (2011) Colchicine inhibits cationic dye uptake induced by ATP in P2X2 and P2X7 receptor-expressing cells: implications for its therapeutic action. Br J Pharmacol 163: 912-926.
37. Tao JH, Zhang Y, Li XP (2013) P2X7R: A potential key regulator of acute gouty arthritis. Seminars in Arthritis and Rheumatism 43: 376-380.