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Volumn 272, Issue 5262, 1996, Pages 735-738

The cytolytic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; PURINE P2 RECEPTOR; PURINE P2X RECEPTOR; 3' O (4 BENZOYL)BENZOYLADENOSINE 5' TRIPHOSPHATE; 3'-O-(4-BENZOYL)BENZOYLADENOSINE 5'-TRIPHOSPHATE; COMPLEMENTARY DNA; DIVALENT CATION; DRUG DERIVATIVE; ION CHANNEL; PURINE P2X7 RECEPTOR;

EID: 0029665112     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5262.735     Document Type: Article
Times cited : (1589)

References (32)
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    • 4- resulting from the addition of 100 μM Na-ATP to these solutions would be 3.7 μM, 5 1 μM, and 29 μM, respectively.
  • 21
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    • note
    • revF/ RT)](20 + 140α)}/145. In order of size (Rg. 3C), X was cesium, methylamine, tris(hydroxymethyl)-aminomethane, tetraethylammonium, and N-methyl-D-glucamine The internal solution also contained 10 mM EGTA and 5 mM Hepes External solutions also contained 10 mM glucose and normal or low concentrations of divalent cations; pH was maintained at 7.3 with HCl, histidine, or Hepes as required, and the osmolarity of all solutions was 295 to 315.
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    • note
    • The induction and kinetics of this sustained current were the same for inward current at - 70 mV and for outward current at 50 mV (n = 3) Indeed, it did not require any current to flow during the first, conditioning agonist applications when BzATP was applied 4 to 12 times in normal divalent solution while the reversal potential was held (0 mV in NaCl or -90 mV in NMDG), the sustained current was still evoked by the subsequent application of BzATP when the low divalent solution was introduced and the holding potential was set to -70 mV (n = 3, A Surprenant et al., data not shown).
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    • YO-PRO-1 (10 μM; Molecular Probes, Eugene, OR) was added to the superfusion fluid during electrophysiological recordings 3 to 6 min before switching to low divalent solution and washed out upon switching back to normal divalent solution, after which the fluorescent lamp was turned on and cells were examined with a fluorescein isothiocyanate filter. For cell counts, 500 cells per cover slip were counted in each case.
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    • note
    • Abbreviations for the amino acid residues are as follows. A, Ala; C, Cys, D, Asp; E, Glu, F, Phe, G, Gly; H, His; I, Ile; K, Lys, L, Leu; M, Met; N, Asn, P, Pro; Q, Gin, R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
  • 32
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    • note
    • 2 cDNA and M J Solazzo for sequencing We are indebted to D Estoppey for cell culture, transfections, and generating stable cell lines.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.