-
2
-
-
0028128589
-
-
H P. Buisman et al , Proc. Natl. Acad. Sci U S.A. 85, 7988 (1988); A. Zambon et al., Cell. Immunol. 156, 458 (1994)
-
(1994)
Cell. Immunol.
, vol.156
, pp. 458
-
-
Zambon, A.1
-
4
-
-
0029064374
-
-
D. K Blanchard et al., Blood 85, 3173 (1995); F. DiVirgilio, P Pizzo, P. Zanovello, V. Bronte, D Collavo, Immunol Today 11, 274 (1990); C. C. Liou C. M. Walsh, J D E. Young, ibid. 16, 194 (1995).
-
(1995)
Blood
, vol.85
, pp. 3173
-
-
Blanchard, D.K.1
-
5
-
-
0025316913
-
-
D. K Blanchard et al., Blood 85, 3173 (1995); F. DiVirgilio, P Pizzo, P. Zanovello, V. Bronte, D Collavo, Immunol Today 11, 274 (1990); C. C. Liou C. M. Walsh, J D E. Young, ibid. 16, 194 (1995).
-
(1990)
Immunol Today
, vol.11
, pp. 274
-
-
DiVirgilio, F.1
Pizzo, P.2
Zanovello, P.3
Bronte, V.4
Collavo, D.5
-
6
-
-
0028961333
-
-
D. K Blanchard et al., Blood 85, 3173 (1995); F. DiVirgilio, P Pizzo, P. Zanovello, V. Bronte, D Collavo, Immunol Today 11, 274 (1990); C. C. Liou C. M. Walsh, J D E. Young, ibid. 16, 194 (1995).
-
(1995)
Immunol Today
, vol.16
, pp. 194
-
-
Liou, C.C.1
Walsh, C.M.2
Young, J.D.E.3
-
9
-
-
0029665752
-
-
G. Collo et al., J. Neurosci 16, 2495 (1996); A. J. Brake, M. J. Wagenbach, D. Julius, Nature 371, 519 (1994); S. Valera et al., ibid , p. 516; C. Chen et al., ibid. 377, 428 (1995), C Lewis et al., ibid , p. 432.
-
(1996)
J. Neurosci
, vol.16
, pp. 2495
-
-
Collo, G.1
-
10
-
-
0028025001
-
-
G. Collo et al., J. Neurosci 16, 2495 (1996); A. J. Brake, M. J. Wagenbach, D. Julius, Nature 371, 519 (1994); S. Valera et al., ibid , p. 516; C. Chen et al., ibid. 377, 428 (1995), C Lewis et al., ibid , p. 432.
-
(1994)
Nature
, vol.371
, pp. 519
-
-
Brake, A.J.1
Wagenbach, M.J.2
Julius, D.3
-
11
-
-
0029665752
-
-
G. Collo et al., J. Neurosci 16, 2495 (1996); A. J. Brake, M. J. Wagenbach, D. Julius, Nature 371, 519 (1994); S. Valera et al., ibid , p. 516; C. Chen et al., ibid. 377, 428 (1995), C Lewis et al., ibid , p. 432.
-
Nature
, pp. 516
-
-
Valera, S.1
-
12
-
-
0029102288
-
-
G. Collo et al., J. Neurosci 16, 2495 (1996); A. J. Brake, M. J. Wagenbach, D. Julius, Nature 371, 519 (1994); S. Valera et al., ibid , p. 516; C. Chen et al., ibid. 377, 428 (1995), C Lewis et al., ibid , p. 432.
-
(1995)
Nature
, vol.377
, pp. 428
-
-
Chen, C.1
-
13
-
-
0029665752
-
-
G. Collo et al., J. Neurosci 16, 2495 (1996); A. J. Brake, M. J. Wagenbach, D. Julius, Nature 371, 519 (1994); S. Valera et al., ibid , p. 516; C. Chen et al., ibid. 377, 428 (1995), C Lewis et al., ibid , p. 432.
-
Nature
, pp. 432
-
-
Lewis, C.1
-
14
-
-
0029671045
-
-
G Buell, C Lewis, G. Collo, R A North, A Surprenant, EMBO J 15, 55 (1996).
-
(1996)
EMBO J
, vol.15
, pp. 55
-
-
Buell, G.1
Lewis, C.2
Collo, G.3
North, R.A.4
Surprenant, A.5
-
15
-
-
5544287416
-
-
note
-
7(1-418).
-
-
-
-
17
-
-
5544220195
-
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note
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+ RNA of each sample that had been electrophoresed and transferred to nylon; hybridization was detected by chemiluminescence
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18
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0020000229
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7 receptors and J774 cells, we constructed concentration-response curves in low divalent solution by recording currents in response to 1-s applications of agonist at 8-min intervals with normal external solution present for 6 min before each application. This protocol was necessary to prevent the development of sustained inward currents (see Figs. 3 and 4) Lipofectin was used for transfection as described (6).
-
(1982)
J Physiol (London)
, vol.331
, pp. 577
-
-
Fenwick, E.M.1
Marty, A.2
Neher, E.3
-
19
-
-
5544328803
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note
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4- resulting from the addition of 100 μM Na-ATP to these solutions would be 3.7 μM, 5 1 μM, and 29 μM, respectively.
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20
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0027530024
-
-
M. Murgia, S Hanau, P Pizzo, M. Rippa, F. DiVirgilio, J. Biol. Chem. 268, 8199 (1993).
-
(1993)
J. Biol. Chem.
, vol.268
, pp. 8199
-
-
Murgia, M.1
Hanau, S.2
Pizzo, P.3
Rippa, M.4
DiVirgilio, F.5
-
21
-
-
5544266063
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note
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revF/ RT)](20 + 140α)}/145. In order of size (Rg. 3C), X was cesium, methylamine, tris(hydroxymethyl)-aminomethane, tetraethylammonium, and N-methyl-D-glucamine The internal solution also contained 10 mM EGTA and 5 mM Hepes External solutions also contained 10 mM glucose and normal or low concentrations of divalent cations; pH was maintained at 7.3 with HCl, histidine, or Hepes as required, and the osmolarity of all solutions was 295 to 315.
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-
-
-
23
-
-
0028072899
-
-
S. E Hickman, J El-Khoury, S Greenberg, I. Schieren, S C. Silverstein, Blood 84, 2452 (1994).
-
(1994)
Blood
, vol.84
, pp. 2452
-
-
Hickman, S.E.1
El-Khoury, J.2
Greenberg, S.3
Schieren, I.4
Silverstein, S.C.5
-
24
-
-
0028182521
-
-
J S. Wiley, J R. Chen, M. B. Snook, G. P Jamieson, Br. J. Pharmacol 112, 946 (1994); T H. Steinberg, A S Newman, J. A. Swanson, S C. Silverstein, J. Biol. Chem. 262, 8884 (1987).
-
(1994)
Br. J. Pharmacol
, vol.112
, pp. 946
-
-
Wiley, J.S.1
Chen, J.R.2
Snook, M.B.3
Jamieson, G.P.4
-
25
-
-
0023664593
-
-
J S. Wiley, J R. Chen, M. B. Snook, G. P Jamieson, Br. J. Pharmacol 112, 946 (1994); T H. Steinberg, A S Newman, J. A. Swanson, S C. Silverstein, J. Biol. Chem. 262, 8884 (1987).
-
(1987)
J. Biol. Chem.
, vol.262
, pp. 8884
-
-
Steinberg, T.H.1
Newman, A.S.2
Swanson, J.A.3
Silverstein, S.C.4
-
26
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5544259000
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note
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The induction and kinetics of this sustained current were the same for inward current at - 70 mV and for outward current at 50 mV (n = 3) Indeed, it did not require any current to flow during the first, conditioning agonist applications when BzATP was applied 4 to 12 times in normal divalent solution while the reversal potential was held (0 mV in NaCl or -90 mV in NMDG), the sustained current was still evoked by the subsequent application of BzATP when the low divalent solution was introduced and the holding potential was set to -70 mV (n = 3, A Surprenant et al., data not shown).
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27
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5544280964
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note
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YO-PRO-1 (10 μM; Molecular Probes, Eugene, OR) was added to the superfusion fluid during electrophysiological recordings 3 to 6 min before switching to low divalent solution and washed out upon switching back to normal divalent solution, after which the fluorescent lamp was turned on and cells were examined with a fluorescein isothiocyanate filter. For cell counts, 500 cells per cover slip were counted in each case.
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29
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5544307367
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data not shown
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A. Surprenant et al., data not shown.
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Surprenant, A.1
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30
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0024093449
-
-
D. W Choi, Neuron 1, 623 (1988)
-
(1988)
Neuron
, vol.1
, pp. 623
-
-
Choi, D.W.1
-
31
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5544289151
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note
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Abbreviations for the amino acid residues are as follows. A, Ala; C, Cys, D, Asp; E, Glu, F, Phe, G, Gly; H, His; I, Ile; K, Lys, L, Leu; M, Met; N, Asn, P, Pro; Q, Gin, R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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32
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5544257296
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note
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2 cDNA and M J Solazzo for sequencing We are indebted to D Estoppey for cell culture, transfections, and generating stable cell lines.
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