DNA nanotechnology-based development of delivery systems for bioactive compounds

European Journal of Pharmaceutical Sciences

Volumn 58, Issue 1, 2014, Pages 26-33

  Mohri, Kohta ^a   Nishikawa, Makiya ^a   Takahashi, Yuki ^a   Takakura, Yoshinobu ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

CpG DNA; DNA nanotechnology; Drug delivery system; Immune response

Indexed keywords

COMPLEMENTARY DNA; CYTOSINE; DINUCLEOTIDE; DNA; DOUBLE STRANDED DNA; DOXORUBICIN; GOLD NANOPARTICLE; GUANINE; INSULIN; NANOMATERIAL; PHOSPHATE; RNA HELICASE; SMALL INTERFERING RNA; TOLL LIKE RECEPTOR 3; TOLL LIKE RECEPTOR 7; TOLL LIKE RECEPTOR 8; TOLL LIKE RECEPTOR 9;

ARTICLE; BIOLOGICAL ACTIVITY; DNA RNA HYBRIDIZATION; DNA STRUCTURE; DRUG DELIVERY SYSTEM; ENZYMATIC DEGRADATION; HUMAN; HYDROGEL; IMMUNOCOMPETENT CELL; INNATE IMMUNITY; NANOTECHNOLOGY; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE;

DNA; DRUG DELIVERY SYSTEMS; HUMANS; IMMUNITY, INNATE; NANOSTRUCTURES; NANOTECHNOLOGY;

EID: 84898933878     PISSN: 09280987     EISSN: 18790720     Source Type: Journal    
DOI: 10.1016/j.ejps.2014.03.002     Document Type: Review

References (102)

1. S. Adams Toll-like receptor agonists in cancer therapy Immunotherapy 1 2009 949 964
2. E.S. Andersen, M. Dong, M.M. Nielsen, K. Jahn, R. Subramani, W. Mamdouh, M.M. Golas, B. Sander, H. Stark, C.L. Oliveira, J.S. Pedersen, V. Birkedal, F. Besenbacher, K.V. Gothelf, and J. Kjems Self-assembly of a nanoscale DNA box with a controllable lid Nature 459 2009 73 76
3. S. Baron, S.K. Tyring, W.R. Fleischmann Jr., D.H. Coppenhaver, D.W. Niesel, G.R. Klimpel, G.J. Stanton, and T.K. Hughes The interferons. Mechanisms of action and clinical applications JAMA 266 1991 1375 1383
4. A.P. Bird CpG islands as gene markers in the vertebrate nucleus Trends Genet. 3 1987 342 347 (Pubitemid 18001851)
5. D.A. Brown, S.H. Kang, S.M. Gryaznov, L. DeDionisio, O. Heidenreich, S. Sullivan, X. Xu, and M.I. Nerenberg Effect of phosphorothioate modification of oligodeoxynucleotides on specific protein binding J. Biol. Chem. 269 1994 26801 26805 (Pubitemid 24332875)
6. W.C. Brown, D.M. Estes, S.E. Chantler, K.A. Kegerreis, and C.E. Suarez DNA and a CpG oligonucleotide derived from Babesia bovis are mitogenic for bovine B cells Infect. Immun. 66 1998 5423 5432 (Pubitemid 29044640)
7. C.E. Castro, F. Kilchherr, D.N. Kim, E.L. Shiao, T. Wauer, P. Wortmann, M. Bathe, and H. Dietz A primer to scaffolded DNA origami Nat. Methods 8 2011 221 229
8. J.H. Chen, and N.C. Seeman Synthesis from DNA of a molecule with the connectivity of a cube Nature 350 1991 631 633 (Pubitemid 21912289)
9. B.C. Chu, and L.E. Orgel The stability of different forms of double-stranded decoy DNA in serum and nuclear extracts Nucleic Acids Res. 20 1992 5857 5858
10. C. Clusel, E. Ugarte, N. Enjolras, M. Vasseur, and M. Blumenfeld Ex vivo regulation of specific gene expression by nanomolar concentration of double-stranded dumbbell oligonucleotides Nucleic Acids Res. 21 1993 3405 3411 (Pubitemid 23241472)
11. F. Crick Central dogma of molecular biology Nature 227 1970 561 563
12. S.T. Crooke Antisense strategies Curr. Mol. Med. 4 2004 465 487 (Pubitemid 38961192)
13. S.T. Crooke Progress in antisense technology Annu. Rev. Med. 55 2004 61 95 (Pubitemid 38316716)
14. S.M. Douglas, I. Bachelet, and G.M. Church A logic-gated nanorobot for targeted transport of molecular payloads Science 335 2012 831 834
15. A. Dove Antisense and sensibility Nat. Biotechnol. 20 2002 121 124 (Pubitemid 34158027)
16. W. Emlen, and M. Mannik Effect of DNA size and strandedness on the in vivo clearance and organ localization of DNA Clin. Exp. Immunol. 56 1984 185 192 (Pubitemid 14161204)
17. E. Fattal, and A. Bochot Ocular delivery of nucleic acids: antisense oligonucleotides, aptamers and siRNA Adv. Drug Deliv. Rev. 58 2006 1203 1223
18. K.O. Freedman, J. Lee, Y. Li, D. Luo, V.B. Skobeleva, and P.C. Ke Diffusion of single star-branched dendrimer-like DNA J. Phys. Chem. B 109 2005 9839 9842 (Pubitemid 40751083)
19. J. Goodchild, B. Kim, and P.C. Zamecnik The clearance and degradation of oligodeoxynucleotides following intravenous injection into rabbits Antisense Res. Dev. 1 1991 153 160
20. R.P. Goodman, R.M. Berry, and A.J. Turberfield The single-step synthesis of a DNA tetrahedron Chem. Commun. 2004 1372 1373 (Pubitemid 38916129)
21. A. Grünweller, E. Wyszko, B. Bieber, R. Jahnel, V.A. Erdmann, and J. Kurreck Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2′-O-methyl RNA, phosphorothioates and small interfering RNA Nucleic Acids Res. 31 2003 3185 3193 (Pubitemid 37441778)
22. M.R. Hartman, R.C. Ruiz, S. Hamada, C. Xu, K.G. Yancey, Y. Yu, W. Han, and D. Luo Point-of-care nucleic acid detection using nanotechnology Nanoscale 5 2013 10141 10154
23. G. Hartmann, J. Battiany, H. Poeck, M. Wagner, M. Kerkmann, N. Lubenow, S. Rothenfusser, and S. Endres Rational design of new CpG oligonucleotides that combine B cell activation with high IFN-alpha induction in plasmacytoid dendritic cells Eur. J. Immunol. 33 2003 1633 1641 (Pubitemid 36790424)
24. Y. He, T. Ye, M. Su, C. Zhang, A.E. Ribbe, W. Jiang, and C. Mao Hierarchical self-assembly of DNA into symmetric supramolecular polyhedral Nature 452 2008 198 201 (Pubitemid 351380239)
25. Y. He, M. Su, P.A. Fang, C. Zhang, A.E. Ribbe, W. Jiang, and C. Mao On the chirality of self-assembled DNA octahedra Angew. Chem. Int. Ed. 49 2010 748 751
26. R. Holliday A mechanism for gene conversion in fungi Genet. Res. 5 1964 282 304
27. V. Hornung, J. Ellegast, S. Kim, K. Brzózka, A. Jung, H. Kato, H. Poeck, S. Akira, K.K. Conzelmann, M. Schlee, S. Endres, and G. Hartmann 5′-Triphosphate RNA is the ligand for RIG-I Science 314 2006 994 997
28. K.J. Ishii, and S. Akira Toll or toll-free adjuvant path toward the optimal vaccine development J. Clin. Immunol. 27 2007 363 371 (Pubitemid 46906656)
29. Q. Jiang, C. Song, J. Nangreave, X. Liu, L. Lin, D. Qiu, Z.G. Wang, G. Zou, X. Liang, H. Yan, and B. Ding DNA origami as a carrier for circumvention of drug resistance J. Am. Chem. Soc. 134 2012 13396 133403
30. N.R. Kallenbach, R.I. Ma, and N.C. Seeman An immobile nucleic acid junction constructed from oligonucleotides Nature 305 1983 829 831 (Pubitemid 13010965)
31. T. Kawai, and S. Akira Innate immune recognition of viral infection Nat. Immunol. 7 2006 131 137 (Pubitemid 43135878)
32. T. Kawai, S. Sato, K.J. Ishii, C. Coban, H. Hemmi, M. Yamamoto, K. Terai, M. Matsuda, J. Inoue, S. Uematsu, O. Takeuchi, and S. Akira Interferon-alpha induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6 Nat. Immunol. 5 2004 1061 1068 (Pubitemid 41057726)
33. M. Kerkmann, L.T. Costa, C. Richter, S. Rothenfusser, J. Battiany, V. Hornung, J. Johnson, S. Englert, T. Ketterer, W. Heckl, S. Thalhammer, S. Endres, and G. Hartmann Spontaneous formation of nucleic acid-based nanoparticles is responsible for high interferon-alpha induction by CpG-A in plasmacytoid dendritic cells J. Biol. Chem. 280 2005 8086 8093 (Pubitemid 40349707)
34. J.W. Keum, J.H. Ahn, and H. Bermudez Design, assembly, and activity of antisense DNA nanostructures Small 7 2011 3529 3535
35. A.M. Krieg CpG motifs in bacterial DNA and their immune effects Annu. Rev. Immunol. 20 2002 709 760 (Pubitemid 34293438)
36. Y. Krishnamachari, and A.K. Salem Innovative strategies for co-delivering antigens and CpG oligonucleotides Adv. Drug Deliv. Rev. 61 2009 205 217
37. A. Krug, S. Rothenfusser, V. Hornung, B. Jahrsdorfer, S. Blackwell, Z.K. Ballas, S. Endres, A.M. Krieg, and G. Hartmann Identification of CpG oligonucleotide sequences with high induction of IFN-alpha/beta in plasmacytoid dendritic cells Eur. J. Immunol. 31 2001 2154 2163 (Pubitemid 32640732)
38. Y. Kuramoto, M. Nishikawa, K. Hyoudou, F. Yamashita, and M. Hashida Inhibition of peritoneal dissemination of tumor cells by single dosing of phosphodiester CpG oligonucleotide/cationic liposome complex J. Control. Release. 115 2006 226 233 (Pubitemid 44537504)
39. G. Lambert, E. Fattal, and P. Couvreur Nanoparticulate systems for the delivery of antisense oligonucleotides Adv. Drug Deliv. Rev. 47 2001 99 112 (Pubitemid 32209235)
40. J.B. Lee, Y.H. Roh, S.H. Um, H. Funabashi, W. Cheng, J.J. Cha, P. Kiatwuthinon, D.A. Muller, and D. Luo Multifunctional nanoarchitectures from DNA-based ABC monomers Nat. Nanotechnol. 4 2009 430 436
41. H. Lee, A.K. Lytton-Jean, Y. Chen, K.T. Love, A.I. Park, E.D. Karagiannis, A. Sehgal, W. Querbes, C.S. Zurenko, M. Jayaraman, C.G. Peng, K. Charisse, A. Borodovsky, M. Manoharan, J.S. Donahoe, J. Truelove, M. Nahrendorf, R. Langer, and D.G. Anderson Molecularly self-assembled nucleic acid nanoparticles for targeted in vivo siRNA delivery Nat. Nanotechnol. 7 2012 389 393
42. J.B. Lee, S. Peng, D. Yang, Y.H. Roh, H. Funabashi, N. Park, E.J. Rice, L. Chen, R. Long, M. Wu, and D. Luo A mechanical metamaterial made from a DNA hydrogel Nat. Nanotechnol. 7 2012 816 820
43. S.J. Lee, M.S. Huh, S.Y. Lee, S. Min, S. Lee, H. Koo, J.U. Chu, K.E. Lee, H. Jeon, Y. Choi, K. Choi, Y. Byun, S.Y. Jeong, K. Park, K. Kim, and I.C. Kwon Tumor-homing poly-siRNA/glycol chitosan self-cross-linked nanoparticles for systemic siRNA delivery in cancer treatment Angew. Chem. Int. Ed. Engl. 51 2012 7203 7207
44. P. Lenert, A.J. Goeken, and R.F. Ashman Extended sequence preferences for oligodeoxyribonucleotide activity Immunology 117 2006 474 481
45. A.G. Leslie, S. Arnott, R. Chandrasekaran, and R.L. Ratliff Polymorphism of DNA double helices J. Mol. Biol. 143 1980 49 72
46. Y. Li, Y.D. Tseng, S.Y. Kwon, L. D'Espaux, J.S. Bunch, P.L. McEuen, and D. Luo Controlled assembly of dendrimer-like DNA Nat. Mater. 3 2004 38 42
47. J. Li, H. Pei, B. Zhu, L. Liang, M. Wei, Y. He, N. Chen, D. Li, Q. Huang, and C. Fan Self-assembled multivalent DNA nanostructures for noninvasive intracellular delivery of immunostimulatory CpGoligonucleotides ACS Nano 5 2011 8783 8789
48. X. Li, C. Zhang, C. Hao, C. Tian, G. Wang, and C. Mao DNA polyhedra with T-linkage ACS Nano 6 2012 5138 5142
49. X. Liu, Y. Xu, T. Yu, C. Clifford, Y. Liu, H. Yan, and Y. Chang A DNA nanostructure platform for directed assembly of synthetic vaccines Nano Lett. 12 2012 4254 4259
50. R.I. Ma, N.R. Kallenbach, R.D. Sheardy, M.L. Petrillo, and N.C. Seeman Three-arm nucleic acid junctions are flexible Nucleic Acids Res. 14 1986 9745 9753
51. Z. Ma, J. Li, F. He, A. Wilson, B. Pitt, and S. Li Cationic lipids enhance siRNA-mediated interferon response in mice Biochem. Biophys. Res. Commun. 330 2005 755 759 (Pubitemid 40462151)
52. J.D. Marshall, K. Fearon, C. Abbate, S. Subramanian, P. Yee, J. Gregorio, R.L. Coffman, and G. Van Nest Identification of a novel CpG DNA class and motif that optimally stimulate B cell and plasmacytoid dendritic cell functions J. Leukocyte Biol. 73 2003 781 792 (Pubitemid 38040457)
53. N. Matsuoka, M. Nishikawa, K. Mohri, S. Rattanakiat, and Y. Takakura Structural and immunostimulatory properties of Y-shaped DNA consisting of phosphodiester and phosphorothioate oligodeoxynucleotides J. Control. Release 148 2010 311 316
54. P. Matzinger Tolerance, danger, and the extended family Annu. Rev. Immunol. 12 1994 991 1045 (Pubitemid 24140436)
55. K. Mohri, M. Nishikawa, N. Takahashi, T. Shiomi, N. Matsuoka, K. Ogawa, M. Endo, K. Hidaka, H. Sugiyama, Y. Takahashi, and Y. Takakura Design and development of nanosized DNA assemblies in polypod-like structures as efficient vehicles for immunostimulatory CpG motifs to immune cells ACS Nano 6 2012 5931 5940
56. K. Mohri, N. Takahashi, M. Nishikawa, E. Kusuki, T. Shiomi, Y. Takahashi, and Y. Takakura Increased immunostimulatory activity of polypod-like structured DNA by ligation of the terminal loop structures J. Control. Release 163 2012 285 292
57. D.K. Monteith, M.J. Horner, N.A. Gillett, M. Butler, R. Geary, T. Burckin, T. Ushiro-Watanabe, and A.A. Levin Evaluation of the renal effects of an antisense phosphorothioate oligodeoxynucleotide in monkeys Toxicol. Pathol. 27 1999 307 317 (Pubitemid 29245041)
58. S. Nayak, and L.A. Lyon Soft nanotechnology with soft nanoparticles Angew. Chem. Int. Ed. Engl. 44 2005 7686 7708 (Pubitemid 41759533)
59. M. Nishikawa, M. Matono, S. Rattanakiat, N. Matsuoka, and Y. Takakura Enhanced immunostimulatory activity of oligodeoxynucleotides by Y-shape formation Immunology 124 2008 247 255 (Pubitemid 351692905)
60. M. Nishikawa, S. Rattanakiat, and Y. Takakura DNA-based nano-sized systems for pharmaceutical and biomedical applications Adv. Drug Deliv. Rev. 62 2010 626 632
61. M. Nishikawa, Y. Mizuno, K. Mohri, N. Matsuoka, S. Rattanakiat, Y. Takahashi, H. Funabashi, D. Luo, and Y. Takakura Biodegradable CpG DNA hydrogels for sustained delivery of doxorubicin and immunostimulatory signals in tumor-bearing mice Biomaterials 32 2011 488 494
62. A. Ozinsky, D.M. Underhill, J.D. Fontenot, A.M. Hajjar, K.D. Smith, C.B. Wilson, L. Schroeder, and A. Aderem The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors Proc. Natl. Acad. Sci. USA 97 2000 13766 13771
63. H. Poeck, M. Wagner, J. Battiany, S. Rothenfusser, D. Wellisch, V. Hornung, B. Jahrsdorfer, T. Giese, S. Endres, and G. Hartmann Plasmacytoid dendritic cells, antigen, and CpG-C license human B cells for plasma cell differentiation and immunoglobulin production in the absence of T-cell help Blood 103 2004 3058 3064 (Pubitemid 38451681)
64. H. Poeck, R. Besch, C. Maihoefer, M. Renn, D. Tormo, S.S. Morskaya, S. Kirschnek, E. Gaffal, J. Landsberg, J. Hellmuth, A. Schmidt, D. Anz, M. Bscheider, T. Schwerd, C. Berking, C. Bourquin, U. Kalinke, E. Kremmer, H. Kato, S. Akira, R. Meyers, G. Häcker, M. Neuenhahn, D. Busch, J. Ruland, S. Rothenfusser, M. Prinz, V. Hornung, S. Endres, T. Tüting, and G. Hartmann 5′-Triphosphate-siRNA: turning gene silencing and Rig-I activation against melanoma Nat. Med. 14 2008 1256 1263
65. S. Rakoff-Nahoum, J. Paglino, F. Eslami-Varzaneh, S. Edberg, and R. Medzhitov Recognition of commensal microflora by Toll-like receptors is required for intestinal homeostasis Cell 118 2004 229 241 (Pubitemid 38962578)
66. R. Rankin, R. Pontarollo, X. Ioannou, A.M. Krieg, R. Hecker, L.A. Babiuk, and S. van Drunen Little-van den Hurk CpG motif identification for veterinary and laboratory species demonstrates that sequence recognition is highly conserved Antisense Nucleic Acid Drug Dev. 11 2001 333 340 (Pubitemid 33070092)
67. S. Rattanakiat, M. Nishikawa, H. Funabashi, D. Luo, and Y. Takakura The assembly of a short linear natural cytosine-phosphate-guanine DNA into dendritic structures and its effect on immunostimulatory activity Biomaterials 30 2009 5701 5706
68. Y.H. Roh, J.B. Lee, P. Kiatwuthinon, M.R. Hartman, J.J. Cha, S.H. Um, D.A. Muller, and D. Luo DNAsomes: multifunctional DNA-based nanocarriers Small 7 2011 74 78
69. Y.H. Roh, R.C. Ruiz, S. Peng, J.B. Lee, and D. Luo Engineering DNA-based functional materials Chem. Soc. Rev. 40 2011 5730 5744
70. P.W. Rothemund Folding DNA to create nanoscale shapes and patterns Nature 440 2006 297 302
71. H. Sands, L.J. Gorey-Feret, A.J. Cocuzza, F.W. Hobbs, D. Chidester, and G.L. Trainor Biodistribution and metabolism of internally 3H-labeled oligonucleotides. I. Comparison of a phosphodiester and a phosphorothioate Mol. Pharmacol. 45 1994 932 943 (Pubitemid 24155784)
72. M. Schlee, A. Roth, V. Hornung, C.A. Hagmann, V. Wimmenauer, W. Barchet, C. Coch, M. Janke, A. Mihailovic, G. Wardle, S. Juranek, H. Kato, T. Kawai, H. Poeck, K.A. Fitzgerald, O. Takeuchi, S. Akira, T. Tuschl, E. Latz, J. Ludwig, and G. Hartmann Recognition of 5′ triphosphate by RIG-I helicase requires short blunt double-stranded RNA as contained in panhandle of negative-strand virus Immunity 31 2009 25 34
73. M. Schmidt, K. Anton, C. Nordhaus, C. Junghans, B. Wittig, and M. Worm Cytokine and Ig-production by CG-containing sequences with phosphorodiester backbone and dumbbell-shape Allergy 61 2006 56 63 (Pubitemid 43381192)
74. V.J. Schüller, S. Heidegger, N. Sandholzer, P.C. Nickels, N.A. Suhartha, S. Endres, C. Bourquin, and T. Liedl Cellular immunostimulation by CpG-sequence-coated DNA origami structures ACS Nano 5 2011 9696 9702
75. N.C. Seeman Nucleic acid junctions and lattices J. Theor. Biol. 99 1982 237 247 (Pubitemid 13215414)
76. N.C. Seeman Nanomaterials based on DNA Annu. Rev. Biochem. 79 2010 65 87
77. H. Shinagawa, and H. Iwasaki Molecular mechanisms of Holliday junction processing in Escherichia coli Adv. Biophys. 31 1995 49 65
78. F.C. Simmel DNA-based assembly lines and nanofactories Curr. Opin. Biotechnol. 23 2012 516 521
79. R. Smolarczyk, T. Cichoń, A. Sochanik, and S. Szala Negligible induction of IFN-gamma, IL-12 and TNF-alpha by DNA-PEI 750 kDa/albumin complexes Cytokine 29 2005 283 287 (Pubitemid 40320929)
80. M.J. Smyth, M. Taniguchi, and S.E. Street The anti-tumor activity of IL-12: mechanisms of innate immunity that are model and dose dependent J. Immunol. 165 2000 2665 2670 (Pubitemid 30660715)
81. C.A. Stein, C. Subasinghe, K. Shinozuka, and J.S. Cohen Physicochemical properties of phosphorothioate oligodeoxynucleotides Nucleic Acids Res. 16 1988 3209 3221
82. M. Swiecki, S.A. McCartney, Y. Wang, and M. Colonna TLR7/9 versus TLR3/MDA5 signaling during virus infections and diabetes J. Leukoc. Biol. 90 2011 691 701
83. Y. Takakura, M. Nishikawa, F. Yamashita, and M. Hashida Development of gene drug delivery systems based on pharmacokinetic studies Eur. J. Pharm. Sci. 13 2001 71 76 (Pubitemid 32259541)
84. A. Takaoka, Z. Wang, M.K. Choi, H. Yanai, H. Negishi, T. Ban, Y. Lu, M. Miyagishi, T. Kodama, K. Honda, Y. Ohba, and T. Taniguchi DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response Nature 448 2007 501 505 (Pubitemid 47123520)
85. T. Tokunaga, S. Yamamoto, and K. Namba A synthetic single-stranded DNA, poly(dG,dC), induces interferon-alpha/beta and -gamma, augments natural killer activity, and suppresses tumor growth Jpn. J. Cancer Res. 79 1988 682 686
86. N. Tomita, T. Ogihara, and R. Morishita Transcription factors as molecular targets: molecular mechanisms of decoy ODN and their design Curr. Drug Targets 4 2003 603 608 (Pubitemid 37236375)
87. C.Y. Tsai, S.L. Lu, C.W. Hu, C.S. Yeh, G.B. Lee, and H.Y. Lei Size-dependent attenuation of TLR9 signaling by gold nanoparticles in macrophages J. Immunol. 188 2012 68 76
88. S. Uematsu, and S. Akira Toll-like receptors and Type I interferons J. Biol. Chem. 282 2007 15319 15323
89. S.H. Um, J.B. Lee, N. Park, S.Y. Kwon, C.C. Umbach, and D. Luo Enzyme-catalysed assembly of DNA hydrogel Nat. Mater. 5 2006 797 801 (Pubitemid 44570861)
90. Uno, S., Nishikawa, M., Mohri, K., Umeki, Y., Matsuzaki, N., Takahashi, Y., Fujita, H., Kadowaki, N., Takakura, Y., Efficient delivery of immunostimulatory DNA to mouse and human immune cells through the construction of polypod-like structured DNA. Nanomedicine, in press.
91. J. Vollmer, R. Weeratna, P. Payette, M. Jurk, C. Schetter, M. Laucht, T. Wader, S. Tluk, M. Liu, H.L. Davis, and A.M. Krieg Characterization of three CpG oligodeoxynucleotide classes with distinct immunostimulatory activities Eur. J. Immunol. 34 2004 251 262 (Pubitemid 38111541)
92. R.W. Wagner Gene inhibition using antisense oligodeoxynucleotides Nature 372 1994 333 335 (Pubitemid 24363432)
93. Z. Wang, M.K. Choi, T. Ban, H. Yanai, H. Negishi, Y. Lu, T. Tamura, A. Takaoka, K. Nishikura, and T. Taniguchi Regulation of innate immune responses by DAI (DLM-1/ZBP1) and other DNA-sensing molecules Proc. Natl. Acad. Sci. USA 105 2008 5477 5482 (Pubitemid 351753888)
94. J.D. Watson, and F.H. Crick Molecular structure of nucleic acids: a structure for deoxyribose nucleic acid Nature 248 1974 765
95. D.Y. Wu, and R.B. Wallace Specificity of the nick-closing activity of bacteriophage T4 DNA ligase Gene 76 1989 245 254 (Pubitemid 19111899)
96. L. Yang, X. Wu, M. Wan, Y. Yu, Y. Yu, and L. Wang CpG oligodeoxynucleotides with double stem-loops show strong immunostimulatory activity Int. Immunopharmacol. 15 2013 89 96
97. A.K. Yi, D.M. Klinman, T.L. Martin, S. Matson, and A.M. Krieg Rapid immune activation by CpG motifs in bacterial DNA. Systemic induction of IL-6 transcription through an antioxidant-sensitive pathway J. Immunol. 157 1996 5394 5402 (Pubitemid 126448958)
98. A.K. Yi, M. Chang, D.W. Peckham, A.M. Krieg, and R.F. Ashman CpG oligodeoxyribonucleotides rescue mature spleen B cells from spontaneous apoptosis and promote cell cycle entry J. Immunol. 160 1998 5898 5906 (Pubitemid 28298049)
99. C. Zhang, M. Su, Y. He, X. Zhao, P.A. Fang, A.E. Ribbe, W. Jiang, and C. Mao Conformational flexibility facilitates self-assembly of complex DNA nanostructures Proc. Natl. Acad. Sci. USA 105 2008 10665 10669
100. C. Zhang, S.H. Ko, M. Su, Y. Leng, A.E. Ribbe, W. Jiang, and C. Mao Symmetry controls the face geometry of DNA polyhedra J. Am. Chem. Soc. 131 2009 1413 1415
101. C. Zhang, M. Su, Y. He, Y. Leng, A.E. Ribbe, G. Wang, W. Jiang, and C. Mao Exterior modification of a DNA tetrahedron Chem. Commun. 46 2010 6792 6794
102. Y.X. Zhao, A. Shaw, X. Zeng, E. Benson, A.M. Nyström, and B. Högberg DNA origami delivery system for cancer therapy with tunable release properties ACS Nano 6 2012 8684 8691