Characterization of three CpG oligodeoxynucleotide classes with distinct immunostimulatory activities

European Journal of Immunology

Volumn 34, Issue 1, 2004, Pages 251-262

  Vollmer, Jörg ^a   Weeratna, Risini ^b   Payette, Paul ^b   Jurk, Marion ^a   Schetter, Christian ^a   Laucht, Meike ^a   Wader, Tanja ^a   Tluk, Sibylle ^a   Liu, Ming ^b   Davis, Heather L ^b   Krieg, Arthur M ^a  

^a PFIZER INC   (United States)

^b NONE   (Canada)

Author keywords

CpG; IFN ; Immunostimulation; Oligodeoxynucleotides; Toll like receptor 9

Indexed keywords

ALPHA INTERFERON; BACTERIAL DNA; CELL SURFACE RECEPTOR; CYTOKINE; DEOXYCYTIDINE; DEOXYGUANOSINE; DINUCLEOTIDE; GAMMA INTERFERON; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; IMMUNOLOGICAL ADJUVANT; MEMBRANE PROTEIN; OLIGODEOXYNUCLEOTIDE; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE; OLIGODEOXYRIBONUCLEOTIDE; PALINDROMIC DNA; TOLL LIKE RECEPTOR; TOLL LIKE RECEPTOR 9;

ANIMAL; ANIMAL CELL; ARTICLE; B LYMPHOCYTE; B LYMPHOCYTE ACTIVATION; BIOSYNTHESIS; CANCER THERAPY; CELL ACTIVATION; CELL STIMULATION; CLASSIFICATION; CONTROLLED STUDY; CPG ISLAND; CYTOKINE PRODUCTION; CYTOKINE RELEASE; DENDRITIC CELL; HUMAN; HUMAN CELL; IMMUNOLOGY; IMMUNOSTIMULATION; IN VIVO STUDY; INFECTION; METABOLISM; METHYLATION; MOLECULAR RECOGNITION; MOUSE; NATURAL KILLER CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN MOTIF; RECEPTOR UPREGULATION; SIGNAL TRANSDUCTION; SPLEEN CELL; TH1 CELL;

ANIMALS; B-LYMPHOCYTES; HUMANS; INTERFERON-ALPHA; MEMBRANE GLYCOPROTEINS; MICE; NF-KAPPA B; OLIGODEOXYRIBONUCLEOTIDES; RECEPTORS, CELL SURFACE; TOLL-LIKE RECEPTOR 9; TOLL-LIKE RECEPTORS;

EID: 9144238063     PISSN: 00142980     EISSN: None     Source Type: Journal    
DOI: 10.1002/eji.200324032     Document Type: Article

References (35)

1. Hemmi, H., Takeuchi, O., Kawai, T., Kaisho, T., Sato, S., Sanjo, H., Matsumoto, M., Hoshino, K., Wagner, H., Takeda, K. and Akira, S., A Toll-like receptor recognizes bacterial DNA. Nature 2000. 408: 740-745.
2. Hemmi, H., Kaisho, T., Takeuchi, O., Sato, S., Sanjo, H., Hoshino, K., Horiuchi, T., Tomizawa, H., Takeda, K. and Akira, S., Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway. Nat. Immunol. 2002. 3: 196-200.
3. Jurk, M., Heil, F, Vollmer, J., Schetter, C., Krieg, A. M., Wagner, H., Lipford, G. and Bauer, S., Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848. Nat. Immunol. 2002. 3: 499.
4. Alexopoulou, L., Holt, A. C., Medzhitov, R. and Flavell, R. A., Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 2001. 413: 732-738.
5. Poltorak, A., He, X., Smirnova, I., Liu, M. Y., Huffel, C. V., Du, X., Birdwell, D., Alejos, E., Silva, M., Galanos, C., Freudenberg, M., Ricciardi-Castagnoli, P., Layton, B. and Beutler, B., Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 1998. 282: 2085-2088.
6. Krieg, A. M., CpG motifs in bacterial DNA and their immune effects. Annu. Rev. Immunol. 2002. 20: 709-760.
7. Krieg, A. M., Yi, A. K., Matson, S., Waldschmidt, T. J., Bishop, G. A., Teasdale, R., Koretzky, G. A. and Klinman, D. M., CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 1995. 374: 546-549.
8. Bauer, S., Kirschning, C. J., Hacker, H., Redecke, V., Hausmann, S., Akira, S., Wagner, H. and Lipford, G. B., Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc. Natl. Acad. Sci. USA 2001. 98: 9237-9242.
9. Hornung, V., Rothenfusser, S., Britsch, S., Krug, A., Jahrsdorfer, B., Giese, T., Endres, S. and Hartmann, G., Quantitative expression of Toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides. J. Immunol. 2002. 168: 4531-4537.
10. Manzel, L., Strekowski, L., Ismail, F. M., Smith, J. C. and Macfarlane, D. E., Antagonism of immunostimulatory CpG-oligodeoxynucleotides by 4-aminoquinolines and other weak bases: mechanistic studies. J. Pharmacol. Exp. Ther. 1999. 291: 1337-1347.
11. Ahmad-Nejad, P., Hacker, H., Rutz, M., Bauer, S., Vabulas, R. M. and Wagner, H., Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur. J. Immunol. 2002. 32: 1958-1968.
12. Manzel, L. and Macfarlane, D. E., Lack of immune stimulation by immobilized CpG-oligodeoxynucleotide. Antisense Nucleic Acid Drug Dev. 1999. 9: 459-464.
13. Takeshita, F., Leifer, C. A., Gursel, I., Ishii, K. J., Takeshita, S., Gursel, M. and Klinman, D. M., Cutting edge: role of Toll-like receptor 9 in CpG DNA-induced activation of human cells. J. Immunol. 2001. 167: 3555-3558.
14. Hartmann, G. and Krieg, A. M., Mechanism and function of a newly identified CpG DNA motif in human primary B cells. J. Immunol. 2000. 164: 944-953.
15. Gursel, M., Verthelyi, D., Gursel, I., Ishii, K. J. and Klinman, D. M., Differential and competitive activation of human immune cells by distinct classes of CpG oligodeoxynucleotide. J. Leukoc. Biol. 2002. 71: 813-820.
16. Hartmann, G., Weeratna, R. D., Ballas, Z. K., Payette, P., Blackwell, S., Suparto, I., Rasmussen, W. L., Waldschmidt, M., Sajuthi, D., Purcell, R. H., Davis, H. L. and Krieg, A. M., Delineation of a CpG phosphorothioate oligodeoxynucleotide for activating primate immune responses in vitro and in vivo. J. Immunol. 2000. 164: 1617-1624.
17. Krug, A., Rothenfusser, S., Hornung, V., Jahrsdorfer, B., Blackwell, S., Ballas, Z. K., Endres, S., Krieg, A. M. and Hartmann, G., Identification of CpG oligonucleotide sequences with high induction of IFN-alpha/beta in plasmacytoid dendritic cells. Eur. J. Immunol. 2001. 31: 2154-2163.
18. Ballas, Z. K., Rasmussen, W. L. and Krieg, A. M., Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA. J. Immunol. 1996. 157: 1840-1845.
19. Verthelyi, D., Ishii, K. J., Gursel, M., Takeshita, F. and Klinman, D. M., Human peripheral blood cells differentially recognize and respond to two distinct CPG motifs. J. Immunol. 2001. 166: 2372-2377.
20. Vollmer, J., Janosch, A., Laucht, M., Ballas, Z. K., Schetter, C. and Krieg, A. M., Highly immunostimulatory CpG-free oligodeoxynucleotides for activation of human leukocytes. Antisense Nucleic Acid Drug Dev. 2002. 12: 165-175.
21. Redford, T W., Yi, A. K., Ward, C. T. and Krieg, A. M., Cyclosporin A enhances IL-12 production by CpG motifs in bacterial DNA and synthetic oligodeoxynucleotides. J. Immunol. 1998. 161: 3930-3935.
22. Uhlmann, E. and Vollmer, J., Recent advances in the development of immunostimulatory oligonucleotides. Curr. Opin. Drug Discov. Devel. 2003. 6. 204-217.
23. Hemmi, H., Kaisho, T, Takeda, K. and Akira, S., The roles of Toll-like receptor 9, MyD88, and DNA-dependent protein kinase catalytic subunit in the effects of two distinct CpG DNAs on dendritic cell subsets. J. Immunol. 2003. 170: 3059-3064.
24. Klinman, D. M., Takeshita, F., Gursel, I., Leifer, C., Ishii, K. J., Verthelyi, D. and Gursel, M., CpG DNA: recognition by and activation of monocytes. Microbes Infect. 2002. 4: 897-901.
25. Hacker, H., Vabulas, R. M., Takeuchi, O., Hoshino, K., Akira, S. and Wagner, H., Immune cell activation by bacterial CpG-DNA through myeloid differentiation marker 88 and tumor necrosis factor receptor-associated factor (TRAF)6. J. Exp. Med. 2000. 192: 595-600.
26. Schnare, M., Holt, A. C., Takeda, K., Akira, S. and Medzhitov, R., Recognition of CpG DNA is mediated by signaling pathways dependent on the adaptor protein MyD88. Curr. Biol. 2000. 10: 1139-1142.
27. Horng, T., Barton, G. M., Flavell, R. A. and Medzhitov, R., The adaptor molecule TIRAP provides signalling specificity for Toll-like receptors. Nature 2002. 420: 329-333.
28. Yamamoto, M., Sato, S., Mori, K., Hoshino, K., Takeuchi, O., Takeda, K. and Akira, S., Cutting edge: a novel Toll/IL-1 receptor domain-containing adapter that preferentially activates the IFN-beta promoter in the Toll-like receptor signaling. J. Immunol. 2002. 169: 6668-6672.
29. Dalpke, A. H., Opper, S., Zimmermann, S. and Heeg, K., Suppressors of cytokine signaling (SOCS)-1 and SOCS-3 are induced by CpG-DNA and modulate cytokine responses in APCs. J. Immunol. 2001. 166: 7082-7089.
30. Hacker, H., Mischak, H., Hacker, G., Eser, S., Prenzal, N., Ullrich, A. and Wagner, H., Cell type-specific activation of mitogen-activated protein kinases by CpG-DNA controls interleukin-12 release from antigen- presenting cells. EMBO J. 1999. 18: 6973-6982.
31. Kobayashi, K., Hernandez, L. D., Galan, J. E., Janeway, C. A., Jr., Medzhitov, R. and Flavell, R. A., IRAK-M is a negative regulator of Toll-like receptor signaling. Cell 2002. 110: 191-202.
32. Davis, H. L., Use of CpG DNA for enhancing specific immune responses. Curr. Top. Microbiol. Immunol. 2000. 247: 171-183.
33. Davis, H. L., Weeratna, R., Waldschmidt, T. J., Tygrett, L., Schorr, J., Krieg, A. M. and Weeranta, R., CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen. J. Immunol. 1998. 160: 870-876.
34. Verthelyi, D., Kenney, R. T., Seder, R. A., Gam, A. A., Friedag, B. and Klinman, D. M., CpG oligodeoxynucleotides as vaccine adjuvants in primates. J. Immunol. 2002. 168: 1659-1663.
35. Blazar, B. R., Krieg, A. M. and Taylor, P. A., Synthetic unmethylated cytosine-phosphate-guanosine oligodeoxynucleotides are potent stimulators of antileukemia responses in naive and bone marrow transplant recipients. Blood 2001. 98: 1217-1225.