Humanization of yeast to produce complex terminally sialylated glycoproteins

Science

Volumn 313, Issue 5792, 2006, Pages 1441-1443

  Hamilton, Stephen R ^a   Davidson, Robert C ^a   Sethuraman, Natarajan ^a   Nett, Juergen H ^a   Jiang, Youwei ^a   Rios, Sandra ^a   Bobrowicz, Piotr ^a   Stadheim, Terrance A ^a   Li, Huijuan ^a   Choi, Byung Kwon ^a   Hopkins, Daniel ^a   Wischnewski, Harry ^a   Roser, Jessica ^a   Mitchell, Teresa ^a   Strawbridge, Rendall R ^b   Hoopes, Jack ^b   Wildt, Stefan ^a   Gerngross, Tillman U ^a,c  

^a GlycoFi Incorporated   (United States)

^b DARTMOUTH HITCHCOCK MEDICAL CENTER   (United States)

^c DARTMOUTH COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; COMPLEXATION; GENES; PROTEINS;

COMPLEX TERMINALLY SIALYLATED GLYCOPROTEINS; GLYCOSYLATION; PICHIA PASTORIS;

YEAST;

GLYCAN; RECOMBINANT ERYTHROPOIETIN; RECOMBINANT PROTEIN; SIALOGLYCOPROTEIN;

GENE; PROTEIN; YEAST;

ARTICLE; GENE REPLICATION; GLYCOSYLATION; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; SIALYLATION; YEAST;

ANIMALS; CELL LINE; CLONING, MOLECULAR; CYTIDINE MONOPHOSPHATE N-ACETYLNEURAMINIC ACID; ERYTHROPOIETIN, RECOMBINANT; GENETIC VECTORS; GLYCOSYLATION; HUMANS; PICHIA; PROTEIN ENGINEERING; RATS; RECOMBINANT PROTEINS; SIALIC ACIDS; SIALOGLYCOPROTEINS; TRANSFORMATION, GENETIC;

PICHIA PASTORIS;

EID: 33748483846     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1130256     Document Type: Article

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13. We thank A. Kull, H. Lynaugh, and A. Thompson for their technical assistance. T.U.G. is a founder of GlycoFi and has equity holdings in Merck. GlycoFi is a wholly owned subsidiary of Merck as of 6 June 2006.