Identifying inhibitory threshold values of repressing metabolites in CHO cell culture using multivariate analysis methods

Biotechnology Progress

Volumn 24, Issue 3, 2008, Pages 675-683

  Xing, Zizhuo ^a   Li, Zhengjian ^a   Chow, Vincent ^b   Lee, Steven S ^a  

^a BRISTOL MYERS SQUIBB COMPANY   (United States)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; AMMONIUM COMPOUNDS; ANIMAL CELL CULTURE; BIOMOLECULES; CARBON; CARBON DIOXIDE; CELL CULTURE; CELL GROWTH; CHEMICAL REACTORS; DECISION MAKING; ENGINEERS; GLOBAL WARMING; GROWTH KINETICS; MAMMALS; METABOLITES; MICROFLUIDICS; MULTIVARIANT ANALYSIS; NUMERICAL METHODS; PHOTOACOUSTIC EFFECT; RANDOM VARIABLES; REGRESSION ANALYSIS; VARIATIONAL TECHNIQUES;

(ALKYL) AMMONIUM; (I ,J) CONDITIONS; AMERICAN CHEMICAL SOCIETY (ACS); AMERICAN INSTITUTE OF CHEMICAL ENGINEERS (AICHE); APPLIED (CO); CANONICAL CORRELATIONS; CELL DENSITIES; CELL-VIABILITY; FED BATCHES; FUSION PROTEINS; LACTATE (LAC); MAMMALIAN CELL CULTURES; MULTIVARIATE ANALYSIS METHODS; OSMOLALITY; PEAK VALUES; PRINCIPAL FACTOR ANALYSIS (PFA); PRODUCT QUALITIES; PROTEIN QUALITY; QUALITY ATTRIBUTES; THRESHOLD VALUE(T);

BATCH CELL CULTURE;

RECOMBINANT PROTEIN;

ANIMAL; BIOLOGICAL MODEL; BIOSYNTHESIS; CHO CELL; COMPUTER SIMULATION; CONFERENCE PAPER; CRICETULUS; CULTURE TECHNIQUE; DIFFERENTIAL THRESHOLD; FEEDBACK SYSTEM; HAMSTER; METABOLISM; METHODOLOGY; MULTIVARIATE ANALYSIS; PHYSIOLOGY;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; COMPUTER SIMULATION; CRICETINAE; CRICETULUS; DIFFERENTIAL THRESHOLD; FEEDBACK; MODELS, BIOLOGICAL; MULTIVARIATE ANALYSIS; RECOMBINANT PROTEINS;

MAMMALIA;

EID: 45149099751     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp070466m     Document Type: Conference Paper

References (39)

1. Farid, S. S. Process economics of industrial monoclonal antibody manufacture. J. Chromatogr. B 2007, 848, 8-18.
2. Jayapal, K. P.; Wlaschin, K. F.; Hu, W. S.; Yap, M. G. S. Recombinant protein therapeutics from CHO cells-20 years and counting. Chem. Eng. Prog. 2007, 103, 40-47.
3. Wurm, F. M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 2004, 22, 1393-1398.
4. Butler, M. Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals. Appl. Microbial. Biotechnol. 2005, 68, 283-291.
5. Butler, M. Optimisation of the cellular metabolism of glycosylation for recombinant proteins produced by mammalian cell systems. Cytotechnology 2006, 50, 57-76.
6. Hassell, T.; Gleave, S.; Butler, M. Growth inhibition in animal cell culture. The effect of lactate and ammonia. Appl. Biochem. Biotechnol., Part A 1991, 30, 29-41.
7. 2 and osmolality: Intracellular pH, cell size, apoptosis, and metabolism. Biotechnol. Bioeng. 2002, 77, 369-380.
8. Ozturk, S. S.; Riley, M. R.; Palsson, B. Ø. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production. Biotechnol. Bioeng. 1992, 39, 418-431.
9. Yang, M.; Butler, M. Effects of ammonia on CHO cell growth, erythropoietin production, and glycosylation. Biotechnol. Bioeng. 2000, 68, 370-380.
10. Lao, M. S.; Toth, D. Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture. Biotechnol. Prog. 1997, 13, 688-691.
11. Zhu, M. M.; Goyal, A.; Rank, D. L.; Gupta, S. K.; Vanden Boom, T.; Lee, S. S. Effects of elevated pCO2 and osmolality on growth of CHO cells and production of antibody-fusion protein B1: a case study. Biotechnol. Prog. 2005, 21, 70-77.
12. deZengotita, V. M.; Abston, L. R.; Schmelzer, A. E.; Shaw, S.; Miller, W. M. Selected amino acids protect hybridoma and CHO cells from elevated carbon dioxide and osmolality. Biotechnol. Bioeng. 2002, 78, 741-752.
13. Shen, D.; Sharfstein, S. Genome-wide analysis of the transcriptional response of murine hybridomas to osmotic shock. Biotechnol. Bioeng. 2006, 93, 132-145.
14. Lee, M. S.; Kim, K. W.; Kim, Y. H.; Lee, G. M. Proteome analysis of antibody-expressing CHO cells in response to hyperosmotic pressure. Biotechnol. Prog. 2003, 19, 1734-1741.
15. Ryu, J. S.; Kim, T. K.; Chung, J. Y.; Lee, G. M. Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant Chinese hamster ovary cell cultures differs among cell lines. Biotechnol. Bioeng. 2000, 70, 167-175.
16. Wong, D. C. F.; Wong, K. T. K.; Goh, L. T.; Heng, C. K.; Yap, M. G. S. Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures. Biotechnol. Bioeng. 2005, 89, 164-177.
17. Altamirano, C.; Illanes, A.; Casablancas, A.; Gámez, X.; Cairó, J. J.; Gòdia, C. Analysis of CHO cells metabolic redistribution in a glutamate-based defined medium in continuous culture. Biotechnol. Prog. 2001, 17, 1032-1041.
18. Altamirano, C.; Illanes, A.; Becerra, S.; Cairó, J. J.; Gòdia, F. Considerations on the lactate consumption by CHO cells in the presence of galactose. J. Biotechnol. 2006, 125, 547-556.
19. 2 removal in large-scale fed-batch cultures. Biotechnol. Prog. 2003, 19, 45-51.
20. Marks, D. M. Equipment design considerations for larger scale cell culture. Cytotechnology 2003, 42, 21-33.
21. Nienow, A. W. Reactor engineering in large scale animal cell culture. Cytotechnology 2006, 50, 9-33.
22. Lilly, M. D. Problems in process scale-up. In Bioactive Microbial Products; Nisbet, L. J., Winstanley, D. J., Eds.; Academic Press: New York, 1983; pp 79-89.
23. Serrato, J. A.; Palomares, L. A.; Meneses-Acosta, A.; Ramirez, O. T. Heterogeneous conditions in dissolved oxygen affect N-glycosylation but not productivity of a monoclonal antibody in hybridoma cultures. Biotechnol. Bioeng. 2004, 88, 176-188.
24. Kirdar, A. O.; Conner, J. S.; Baclaski, J.; Rathore, A. S. Application of multivariate analysis toward biotech processes: case study of a cell-culture unit operation. Biotechnol. Prog. 2007, 23, 61-67.
25. Bylund, F.; Collet, E.; Enfors, S. O.; Larsson, G. Substrate gradient formation in the large-scale bioreactor lowers cell yield and increases by-product formation. Bioprocess Eng. 1998, 18, 171-180.
26. Amanullah, A.; McFarlane, C. M.; Emery, A. N.; Nienow, A. W. Scale-down model to simulate spatial pH variations in large-scale bioreactors. Biotechnol. Bioeng. 2001, 73, 390-399.
27. Portner, R.; Schäfer, T. Modelling hybridoma cell growth and metabolism - a comparison of selected models and data, J. Biotechnol. 1996, 49, 119-135.
28. Batt, B. C.; Kompala, D. S. A structured kinetic modeling framework for the dynamics of Hybridoma growth and monoclonal antibody production in continuous suspension culture, Biotechnol. Bioeng. 1989, 34, 15-531.
29. Bree, M. A.; Dhurjati, P. Kinetic modeling of hybridoma cell growth and immunoglobulin production in a large-scale suspension culture, Biotechnol. Bioeng. 1988, 32, 1067-1072.
30. Miller, W. M; Blanch, H. W.; Wilke, C. R. A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: effect of nutrient concentration, dilution rate, and pH. Biotechnol. Bioeng. 1988, 32, 947-965.
31. Lennox, B.; Kipling, K.; Glassey, J.; Montague, G.; Willis, M.; Hiden, H. Automated production support for the bioprocess industry. Biotechnol. Prog. 2002, 18, 269-275.
32. De Alwis, D. M.; Dutton, R. L.; Scharer, J.; Moo-Young, M. Statistical methods in media optimization for batch and fed-batch animal cell culture. Bioprocess Biosyst. Eng. 2007, 30, 107-113.
33. Heinen, A.; Rengifo, E. Multivariate reduced rank regression in non-Gaussian contexts, using copulas. Comput. Statist. Data Anal. 2008, 52, 2931-2944.
34. Manly, B. F. J. Multivariate Statistical Methods: A Primer; Chapman & Hall: London, 1991.
35. Stevens, J. Applied Multivariate Statistics for the Social Sciences; Lawrence Erlbaum Associates: Mahwah, NJ, 2002.
36. Namdev, P. K.; Yegneswaran, P. K.; Thompson, B. G.; Gray, M. R. Experimental simulation of large-scale bioreactor environments using a Monte Carlo method. Can. J. Chem. Eng. 1991, 69, 513-519.
37. Zar, J. H. Biostatistical Analysis; Prentice Hall Inc.: Englewood Cliffs, NJ, 1984.
38. Frame, K. K.; Hu, W-S. Kinetic study of hybridoma cell growth in continuous culture. I. A model for non-producing cells. Biotechnol. Bioeng. 1991, 37, 55-64.
39. 2 in large-scale and high-density CHO cell perfusion culture. Cytotechnology 1996, 22, 65-78.