Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection

Cytotechnology

Volumn 60, Issue 1-3, 2009, Pages 115-123

  Reisinger, Hannes ^a   Steinfellner, Willibald ^a   Katinger, Hermann ^a,b   Kunert, Renate ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b POLYMUN SCIENTIFIC IMMUNBIOLOGISCHE FORSCHUNG GMBH   (Austria)

Author keywords

Antibody expression; DOTAP DOPE; PEI; Serum free transfection

Indexed keywords

1,2 DIOLEOYL 3 TRIMETHYLAMMONIOPROPANE; 3,4 DIHYDROXYPHENYLETHYLENE GLYCOL; ENHANCED GREEN FLUORESCENT PROTEIN; METHOTREXATE; MONOCLONAL ANTIBODY 2F5; POLYETHYLENEIMINE;

ARTICLE; CELL COUNT; CELL CYCLE; CELL EXPANSION; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PROTEIN EXPRESSION; SCREENING; TRANSIENT EXPRESSION;

EID: 71349086605     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-009-9224-x     Document Type: Article

References (29)

1. FW Alt RE Kellems JR Bertino RT Schimke 1978 Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells J Biol Chem 253 1357 1370 1:CAS:528:DyaE1cXhtlCmsbc%3D
2. Backliwal G, Hildinger M, Chenuet S, DeJesus M, Wurm F (2008) Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells. Nat Biotechnol 25:162-166
3. JY Cherng NM Schuurmans-Nieuwenbroek W Jiskoot H Talsma NJ Zuidam WE Hennink DJ Crommelin 1999 Effect of DNA topology on the transfection efficiency of poly((2-dimethylamino)ethyl methacrylate)-plasmid complexes J Control Release 60 343 353 10.1016/S0168-3659(99)00089-9 1:CAS:528:DyaK1MXks1Gjs70%3D (Pubitemid 29335959)
4. V Escriou M Carriere F Bussone P Wils D Scherman 2001 Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer J Gene Med 3 179 187 10.1002/jgm.174 1:STN:280:DC%2BD3MvkslGkug%3D%3D (Pubitemid 33685445)
5. DJ Galbraith AS Tait AJ Racher JR Birch DC James 2006 Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells Biotechnol Prog 22 753 762 10.1021/bp050339v 1:CAS:528:DC%2BD28XitlSktLo%3D (Pubitemid 43877444)
6. WT Godbey KK Wu GJ Hirasaki AG Mikos 1999 Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency Gene Ther 6 1380 1388 10.1038/sj.gt.3300976 1:CAS:528:DyaK1MXlt1Cis7o%3D (Pubitemid 29396726)
7. BA Jardin Y Zhao M Selvaraj J Montes R Tran S Prakash CB Elias 2008 Expression of SEAP (secreted alkaline phosphatase) by baculovirus mediated transduction of HEK 293 cells in a hollow fiber bioreactor system J Biotechnol 135 272 280 10.1016/j.jbiotec.2008.04.006 1:CAS:528:DC%2BD1cXns1SmsLc%3D
8. IA Khalil K Kogure H Akita H Harashima 2006 Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery Pharmacol Rev 58 32 45 10.1124/pr.58.1.8 1:CAS:528:DC%2BD28XjtlCqsLw%3D (Pubitemid 43313695)
9. R Kunert F Ruker H Katinger 1998 Molecular characterization of five neutralizing anti-HIV type 1 antibodies: identification of nonconventional D segments in the human monoclonal antibodies 2G12 and 2F5 AIDS Res Hum Retroviruses 14 1115 1128 10.1089/aid.1998.14.1115 1:CAS:528:DyaK1cXmtFSms74%3D
10. R Kunert W Steinfellner M Purtscher A Assadian H Katinger 2000 Stable recombinant expression of the anti HIV-1 monoclonal antibody 2F5 after IgG3/IgG1 subclass switch in CHO cells Biotechnol Bioeng 67 97 103 10.1002/(SICI)1097- 0290(20000105)67:1<97::AID-BIT11>3.0.CO;2-2 1:CAS:528:DC%2BD3cXltlej (Pubitemid 30029430)
11. PU Le IR Nabi 2003 Distinct caveolae-mediated endocytic pathways target the golgi apparatus and the endoplasmic reticulum J Cell Sci 116 1059 1071 10.1242/jcs.00327 1:CAS:528:DC%2BD3sXis1antrg%3D (Pubitemid 36395616)
12. DJ Leisy TD Lewis JA Leong GF Rohrmann 2003 Transduction of cultured fish cells with recombinant baculoviruses J Gen Virol 84 1173 1178 10.1099/vir.0.18861-0 1:CAS:528:DC%2BD3sXjsFCrsbw%3D (Pubitemid 36592610)
13. AJ Mahonen KJ Airenne S Purola E Peltomaa MU Kaikkonen MS Riekkinen T Heikura K Kinnunen MM Roschier T Wirth, et al. 2007 Post-transcriptional regulatory element boosts baculovirus-mediated gene expression in vertebrate cells J Biotechnol 131 1 8 10.1016/j.jbiotec.2007.05.022 (Pubitemid 47042932)
14. N Muller P Girard DL Hacker M Jordan FM Wurm 2005 Orbital shaker technology for the cultivation of mammalian cells in suspension Biotechnol Bioeng 89 400 406 10.1002/bit.20358 1:CAS:528:DC%2BD2MXhtFektL0%3D (Pubitemid 40288900)
15. N Muller M Derouazi F Van Tilborgh S Wulhfard DL Hacker M Jordan FM Wurm 2007 Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems Biotechnol Lett 29 703 711 10.1007/s10529-006-9298-x 1:CAS:528:DC%2BD2sXjvFagu78%3D (Pubitemid 46569215)
16. W Ping J Ge S Li H Zhou K Wang Y Feng Z Lou 2006 Baculovirus-mediated gene expression in chicken primary cells Avian Dis 50 59 63 10.1637/7418- 080705R.1
17. C Plank K Mechtler FC Szoka Jr E Wagner 1996 Activation of the complement system by synthetic DNA complexes: a potential barrier for intravenous gene delivery Hum Gene Ther 7 1437 1446 10.1089/hum.1996.7.12-1437 1:CAS:528:DyaK28Xltlaltbs%3D
18. AE Regelin S Fankhaenel L Gurtesch C Prinz G von Kiedrowski U Massing 2000 Biophysical and lipofection studies of DOTAP analogs Biochim Biophys Acta 1464 151 164 10.1016/S0005-2736(00)00126-7 1:CAS:528:DC%2BD3cXhsFGkt70%3D (Pubitemid 30110526)
19. H Reisinger E Sevcsik K Vorauer-Uhl K Lohner H Katinger R Kunert 2007 Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles Cytotechnology 54 157 168 10.1007/s10616-007-9070-7 (Pubitemid 47339779)
20. A Spenger W Ernst JP Condreay TA Kost R Grabherr 2004 Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells Protein Expr Purif 38 17 23 10.1016/j.pep.2004.08.001 1:CAS:528:DC%2BD2cXotlSlt7s%3D (Pubitemid 39348446)
21. K Takei V Haucke 2001 Clathrin-mediated endocytosis: membrane factors pull the trigger Trends Cell Biol 11 385 391 10.1016/S0962-8924(01)02082-7 1:CAS:528:DC%2BD3MXmtV2gsLY%3D (Pubitemid 32900531)
22. JD Tousignant AL Gates LA Ingram CL Johnson JB Nietupski SH Cheng SJ Eastman RK Scheule 2000 Comprehensive analysis of the acute toxicities induced by systemic administration of cationic lipid: plasmid DNA complexes in mice Hum Gene Ther 11 2493 2513 10.1089/10430340050207984 1:CAS:528:DC%2BD3cXptVKmt78%3D
23. G Urlaub LA Chasin 1980 Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity Proc Natl Acad Sci U S A 77 4216 4220 10.1073/pnas.77.7.4216 1:CAS:528:DyaL3cXltFWku7o%3D (Pubitemid 10022580)
24. MA van der Aa US Huth SY Hafele R Schubert RS Oosting E Mastrobattista WE Hennink R Peschka-Suss GA Koning DJ Crommelin 2007 Cellular uptake of cationic polymer-DNA complexes via caveolae plays a pivotal role in gene transfection in COS-7 cells Pharm Res 24 1590 1598 10.1007/s11095-007-9287-3 1:CAS:528:DC%2BD2sXmvVyqsbs%3D (Pubitemid 46988225)
25. R Wattiaux M Jadot MT Warnier-Pirotte S Wattiaux-De Coninck 1997 Cationic lipids destabilize lysosomal membrane in vitro FEBS Lett 417 199 202 10.1016/S0014-5793(97)01283-0 1:CAS:528:DyaK2sXnt1ektr0%3D (Pubitemid 27491732)
26. R Wattiaux N Laurent S Wattiaux-De Coninck M Jadot 2000 Endosomes, lysosomes: their implication in gene transfer Adv Drug Deliv Rev 41 201 208 10.1016/S0169-409X(99)00066-6 1:CAS:528:DC%2BD3cXhsVWqsb4%3D (Pubitemid 30122959)
27. I Wrobel D Collins 1995 Fusion of cationic liposomes with mammalian cells occurs after endocytosis Biochim Biophys Acta 1235 296 304 10.1016/0005- 2736(95)80017-A
28. FM Wurm 2004 Production of recombinant protein therapeutics in cultivated mammalian cells Nat Biotechnol 22 1393 1398 10.1038/nbt1026 1:CAS:528:DC%2BD2cXptlSls7k%3D (Pubitemid 39482858)
29. IS Zuhorn U Bakowsky E Polushkin WH Visser MC Stuart JB Engberts D Hoekstra 2005 Nonbilayer phase of lipoplex-membrane mixture determines endosomal escape of genetic cargo and transfection efficiency Mol Ther 11 801 810 10.1016/j.ymthe.2004.12.018 1:CAS:528:DC%2BD2MXjsFGqtrw%3D (Pubitemid 40567984)