Giardia/giardiasis - A perspective on diagnostic and analytical tools

Biotechnology Advances

Volumn 32, Issue 2, 2014, Pages 280-289

  Koehler, Anson V ^a   Jex, Aaron R ^a   Haydon, Shane R ^b   Stevens, Melita A ^b   Gasser, Robin B ^a,c  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b MELBOURNE WATER CORPORATION   (Australia)

^c Inst Parasitol and Trop Vet Med   (Germany)

Author keywords

Detection; Diagnosis; Genetic characterisation; Giardia; Giardia duodenalis; Giardiasis; Metamonada; Protista

Indexed keywords

DISEASES; ERROR DETECTION; GENES; PROTOZOA;

GENETIC CHARACTERISATION; GIARDIA; GIARDIA DUODENALIS; GIARDIASIS; METAMONADA; PROTISTA;

DIAGNOSIS;

EPIDEMIOLOGY; GASTROENTERITIS; GENETIC ANALYSIS; GENETIC VARIATION; GENOTYPE; PARASITIC DISEASE; PROTIST; SOCIOECONOMIC STATUS;

ANIMALIA; GIARDIA; GIARDIA INTESTINALIS; METAMONADA; PROTISTA;

ANIMAL; DETECTION; DIAGNOSIS; GENETIC CHARACTERISATION; GENETICS; GIARDIA; GIARDIA LAMBLIA; GIARDIASIS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METAMONADA; METHODOLOGY; MOUSE; PARASITOLOGY; PROTIST; REVIEW;

DETECTION; DIAGNOSIS; GENETIC CHARACTERISATION; GIARDIA; GIARDIA DUODENALIS; GIARDIASIS; METAMONADA; PROTISTA;

ANIMALS; GIARDIA; GIARDIASIS; HUMANS; MICE; PARASITOLOGY;

EID: 84895473135     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2013.10.009     Document Type: Review

References (219)

1. Abbaszadegan M., Huber M.S., Gerba C.P., Pepper I.L. Detection of viable Giardia cysts by amplification of heat shock-induced mRNA. Appl Environ Microbiol 1997, 63:324-328.
2. Abeywardena H., Jex A.R., Nolan M.J., Haydon S.R., Stevens M.A., McAnulty R.W., et al. Genetic characterisation of Cryptosporidium and Giardia from dairy calves: discovery of species/genotypes consistent with those found in humans. Infect Genet Evol 2012, 12:1984-1993.
3. Adam R.D. Biology of Giardia lamblia. Clin Microbiol Rev 2001, 14:447-475.
4. Adamska M., Leonska-Duniec A., Maciejewska A., Sawczuk M., Skotarczak B. Comparison of efficiency of various DNA extraction methods from cysts of Giardia intestinalis measured by PCR and TaqMan real time PCR. Parasite 2010, 17:299.
5. Addiss D.G., Mathews H.M., Stewart J.M., Wahlquist S.P., Williams R.M., Finton R.J., et al. Evaluation of a commercially available enzyme-linked immunosorbent assay for Giardia lamblia antigen in stool. J Clin Microbiol 1991, 29:1137-1142.
6. Aldeen W.E., Carroll K., Robison A., Morrison M., Hale D. Comparison of nine commercially available enzyme-linked immunosorbent assays for detection of Giardia lamblia in fecal specimens. J Clin Microbiol 1998, 36:1338-1340.
7. Aldeen W.E., Hale D., Robison A.J., Carroll K. Evaluation of a commercially available ELISA assay for detection of Giardia lamblia in fecal specimens. Diagn Microbiol Infect Dis 1995, 21:77-79.
8. Alles A.J., Waldron M.A., Sierra L.S., Mattia A.R. Prospective comparison of direct immunofluorescence and conventional staining methods for detection of Giardia and Cryptosporidium spp. in human fecal specimens. J Clin Microbiol 1995, 33:1632-1634.
9. Almeida A., Pozio E., Cacciò S.M. Genotyping of Giardia duodenalis cysts by new real-time PCR assays for detection of mixed infections in human samples. Appl Environ Microbiol 2010, 76:1895-1901.
10. Alonso J.L., Amorós I., Cañigral I. Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples. Appl Microbiol Biotechnol 2011, 89:1203-1211.
11. Alonso J.L., Amoros I., Cuesta G. LNA probes in a real-time TaqMan PCR assay for genotyping of Giardia duodenalis in wastewaters. J Appl Microbiol 2010, 108:1594-1601.
12. Amann R., Ludwig W. Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology. FEMS Microbiol Rev 2000, 24:555-565.
13. Amann R.I., Binder B.J., Olson R.J., Chisholm S.W., Devereux R., Stahl D.A. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 1990, 56:1919-1925.
14. Amann R.I., Ludwig W., Schleifer K.-H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 1995, 59:143-169.
15. Amar C.F.L. Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from human faeces. J Med Microbiol 2003, 52:681-683.
16. Amar C.F.L., Dear P.H., Pedraza-Diaz S., Looker N., Linnane E., McLauchlin J. Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces. J Clin Microbiol 2002, 40:446-452.
17. Ament M.E. Diagnosis and treatment of giardiasis. J Pediatr 1972, 80:633-637.
18. Anceno A.J., Katayama H., Houpt E.R., Chavalitshewinkoon-Petmitr P., Chuluun B., Shipin O.V. IMS-free DNA extraction for the PCR-based quantification of Cryptosporidium parvum and Giardia lamblia in surface and waste water. Int J Environ Health Res 2007, 17:297-310.
19. Ankarklev J., Jerlström-Hultqvist J., Ringqvist E., Troell K., Svärd S.G. Behind the smile: cell biology and disease mechanisms of Giardia species. Nature Rev Microbiol 2010, 8:413-422.
20. Appelbee A.J., Thompson R.C.A., Olson M.E. Giardia and Cryptosporidium in mammalian wildlife - current status and future needs. Trends Parasitol 2005, 21:370-376.
21. Asher A.J., Waldron L.S., Power M.L. Evaluation of a PCR protocol for sensitive detection of Giardia intestinalis in human faeces. Parasitol Res 2012, 110:853-858.
22. Asher A.J., Waldron L.S., Power M.L. Rapid identification of Giardia duodenalis assemblages in NSW using terminal-restriction fragment length polymorphism. Parasitology 2012, 139:1005-1013.
23. ASTM. American Society for Testing and Materials. Philadelphia; 1991. p. 925-35.
24. Aziz H., Beck C.E., Lux M.F., Hudson M.J. A comparison study of different methods used in the detection of Giardia lamblia. Clin Lab Sci 2001, 14:150.
25. Babaei Z., Oormazdi H., Rezaie S., Rezaeian M., Razmjou E. Giardia intestinalis: DNA extraction approaches to improve PCR results. Exp Parasitol 2011, 128:159-162.
26. Baig M.F., Kharal S.A., Qadeer S.A., Badvi J.A. A comparative study of different methods used in the detection of Giardia lamblia on fecal specimens of children. Ann Trop Med Public Health 2012, 5:163-167.
27. Baker D.A., Holberton D.V., Marshall J. Sequence of a giardin subunit cDNA from Giardia lamblia. Nucleic Acids Res 1988, 16:7177.
28. Baldursson S., Karanis P. Waterborne transmission of protozoan parasites: review of worldwide outbreaks - an update 2004-2010. Water Res 2011, 45:6603-6614.
29. Baque R.H., Gilliam A.O., Robles L.D., Jakubowski W., Slifko T.R. A real-time RT-PCR method to detect viable Giardia lamblia cysts in environmental waters. Water Res 2011, 45:3175-3184.
30. Barbosa J., Costa-de-Oliveira S., Rodrigues A.G., Pina-Vaz C. Optimization of a flow cytometry protocol for detection and viability assessment of Giardia lamblia. Travel Med Infect Dis 2008, 6:234-239.
31. Baruch A.C., Isaac-Renton J., Adam R.D. The molecular epidemiology of Giardia lamblia: a sequence-based approach. J Infect Dis 1996, 174:233-236.
32. Beck R., Sprong H., Bata I., Lucinger S., Pozio E., Cacciò S.M. Prevalence and molecular typing of Giardia spp. in captive mammals at the zoo of Zagreb, Croatia. Vet Parasitol 2011, 175:40-46.
33. Beck R., Sprong H., Lucinger S., Pozio E., Cacciò S.M. A large survey of Croatian wild mammals for Giardia duodenalis reveals a low prevalence and limited zoonotic potential. Vector Borne Zoonotic Dis 2011, 11:1049-1055.
34. Beck R., Sprong H., Pozio E., Cacciò S.M. Genotyping Giardia duodenalis isolates from dogs: lessons from a multilocus sequence typing study. Vector Borne Zoonotic Dis 2012, 12:206-213.
35. Bednarska M., Bajer A., Sinski E., Girouard A.S., Tamang L., Graczyk T.K. Fluorescent in situ hybridization as a tool to retrospectively identify Cryptosporidium parvum and Giardia lamblia in samples from terrestrial mammalian wildlife. Parasitol Res 2007, 100:455-460.
36. Behr M.A., Kokoskin E., Gyorkos T.W., Cédilotte L., Faubert G.M., MacLean J.D. Laboratory diagnosis for Giardia lamblia infection: a comparison of microscopy, coprodiagnosis and serology. Can J Infect Dis 1997, 8:33-38.
37. Bertrand I., Gantzer C., Chesnot T., Schwartzbrod J. Improved specificity for Giardia lamblia cyst quantification in wastewater by development of a real-time PCR method. J Microbiol Meth 2004, 57:41-53.
38. Bertrand I., Maux M., Helmi K., Hoffmann L., Schwartzbrod J., Cauchie H.-M. Quantification of Giardia transcripts during in vitro excystation: interest for the estimation of cyst viability. Water Res 2009, 43:2728-2738.
39. Betancourt W.Q., Rose J.B. Drinking water treatment processes for removal of Cryptosporidium and Giardia. Vet Parasitol 2004, 126:219-234.
40. Buret A.G., Cotton J. Pathophysiological processes and clinical manifestations of giardiasis. Svärd S, editors 2011, 301-318. Springer, Giardia. H.D. Luján (Ed.).
41. Cacciò S.M., Beck R., Almeida A., Bajer A., Pozio E. Identification of Giardia species and Giardia duodenalis assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers. Parasitology 2010, 137:919-925.
42. Cacciò S.M., Beck R., Lalle M., Marinculic A., Pozio E. Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. Int J Parasitol 2008, 38:1523-1531.
43. Cacciò S.M., Ryan U.M. Molecular epidemiology of giardiasis. Mol Biochem Parasitol 2008, 160:75-80.
44. Chan R., Chen J., York M.K., Setijono N., Kaplan R.L., Graham F., et al. Evaluation of a combination rapid immunoassay for detection of Giardia and Cryptosporidium antigens. J Clin Microbiol 2000, 38:393-394.
45. Cole L., Schupp D.G., Erlandsen S.L. Viability of Giardia cysts suspended in lake, river, and tap water. Appl Environ Microbiol 1989, 55:1223-1229.
46. Cooper M.A., Adam R.D., Worobey M., Sterling C.R. Population genetics provides evidence for recombination in Giardia. Curr Biol 2007, 17:1984-1988.
47. Costache C.A., Colosi I.A., Colosi H.A. Immunochromatography versus microscopy for the identification of Giardia lamblia and Cryptosporidium parvum in human feces. Sci Parasitol 2009, 10:26-31.
48. de Souza W., Lanfredi-Rangel A., Campanati L. Contribution of microscopy to a better knowledge of the biology of Giardia lamblia. Microsc Microanal 2004, 10:513-527.
49. Deng M.-Q., Cliver D.O. Rapid DNA extraction methods and new primers for randomly amplified polymorphic DNA analysis of Giardia duodenalis. J Microbiol Meth 1999, 37:193-200.
50. DiGiorgio C.L., Gonzalez D.A., Huitt C.C. Cryptosporidium and Giardia recoveries in natural waters by using Environmental Protection Agency Method 1623. Appl Environ Microbiol 2002, 68:5952-5955.
51. Dixon B.R., Parenteau M., Martineau C., Fournier J. A comparison of conventional microscopy, immunofluorescence microscopy and flow cytometry in the detection of Giardia lamblia cysts in beaver fecal samples. J Immunol Meth 1997, 202:27-33.
52. Dorsch M.R., Veal D.A. Oligonucleotide probes for specific detection of Giardia lamblia cysts by fluorescent in situ hybridization. J Appl Microbiol 2001, 90:836-842.
53. Ellsworth D.L., Rittenhouse K.D., Honeycutt R.L. Artifactual variation in randomly amplified polymorphic DNA banding patterns. BioTechniques 1993, 14:214-217.
54. Elsafi S.H., Al-Maqati T.N., Hussein M.I., Adam A.A., Hassan M.M.A., Al Zahrani E.M. Comparison of microscopy, rapid immunoassay, and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum. Parasitol Res 2013, 112:1641-1646.
55. Elwakil H.S., Ahmed M.A. A single-step immunochromatographic lateral-flow assay for detection of Giardia lamblia and Cryptosporidium parvum antigens in human fecal samples. J Egypt Soc Parasitol 2008, 38:797-804.
56. Erlandsen S.L., Jarroll E., Wallis P., van Keulen H. Development of species-specific rDNA probes for Giardia by multiple fluorescent in situ hybridization combined with immunocytochemical identification of cyst wall antigens. J Histochem Cytochem 2005, 53:917-927.
57. Erlandsen S.L., VanKeulen H., Gurien A., Jakubowski W., Schafer F.W., Wallis P. Molecular approach to speciation and detection of Giardia: fluorochrome rDNA probes for identification of Giardia lamblia, Giardia muris, and Giardia ardeae in laboratory and environmental samples by in situ hybridization. Giardia: from molecules to disease 1994, 64-65. CAB International, Oxford. R.C.A. Thompson, J.A. Reynoldson, A.J. Lymbery (Eds.).
58. Escobedo A.A., Cimerman S. Giardiasis: a pharmacotherapy review. Expert Opin Pharmacotherapy 2007, 8:1885-1902.
59. Farthing M., Mata L., Urrutia J.J., Kronmal R.A. Natural history of Giardia infection of infants and children in rural Guatemala and its impact on physical growth. Am J Clin Nutr 1986, 43:395-405.
60. Fedorko D.P., Williams E.C., Nelson N.A., Calhoun L.B., Yan S.S. Performance of three enzyme immunoassays and two direct fluorescence assays for detection of Giardia lamblia in stool specimens preserved in ECOFIX. J Clin Microbiol 2000, 38:2781-2783.
61. Feng Y., Xiao L. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev 2011, 24:110-140.
62. Feng Y., Xiao L. Giardia. Molecular detection of human parasitic pathogens 2012, 77-90. CRC Press, Boca Raton. D. Liu (Ed.).
63. Ferguson C., Kaucner C., Krogh M., Deere D., Warnecke M. Comparison of methods for the concentration of Cryptosporidium oocysts and Giardia cysts from raw waters. Can J Microbiol 2004, 50:675-682.
64. Ferrari B.C., Veal D. Analysis-only detection of Giardia by combining immunomagnetic separation and two-color flow cytometry. Cytometry A 2003, 51:79-86.
65. Filice F.P. Studies on the cytology and life history of a Giardia from the laboratory rat. U Calif Pub Zool 1952, 57:53-146.
66. Fletcher S.M., Stark D., Harkness J., Ellis J. Enteric protozoa in the developed world: a public health perspective. Clin Microbiol Rev 2012, 25:420-449.
67. Franzen O., Jerlstrom-Hultqvist J., Castro E., Sherwood E., Ankarklev J., Reiner D.S., et al. Draft genome sequencing of Giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?. PLoS Pathog 2009, 5:e1000560.
68. Garcia L.S. Diagnostic medical parasitology: ASM Press 2007.
69. Garcia L.S. Practical guide to diagnostic parasitology 2009, ASM Press, Washington, DC. 2nd ed.
70. Garcia L.S., Garcia J.P. Detection of Giardia lamblia antigens in human fecal specimens by a solid-phase qualitative immunochromatographic assay. J Clin Microbiol 2006, 44:4587-4588.
71. Garcia L.S., Shimizu R.Y. Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens. J Clin Microbiol 1997, 35:1526-1529.
72. Garcia L.S., Shimizu R.Y., Novak S., Carroll M., Chan F. Commercial assay for detection of Giardia lamblia and Cryptosporidium parvum antigens in human fecal specimens by rapid solid-phase qualitative immunochromatography. J Clin Microbiol 2003, 41:209-212.
73. Gardner T.B., Hill D.R. Treatment of giardiasis. Clin Microbiol Rev 2001, 14:114-128.
74. Gasser R.B. Molecular tools - advances, opportunities and prospects. Vet Parasitol 2006, 136:69-89.
75. Gasser R.B., Hu M., Chilton N.B., Campbell B.E., Jex A.J., Otranto D., et al. Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation. Nat Protoc 2006, 1:3121-3128.
76. Geurden T., Berkvens D., Casaert S., Vercruysse J., Claerebout E. A Bayesian evaluation of three diagnostic assays for the detection of Giardia duodenalis in symptomatic and asymptomatic dogs. Vet Parasitol 2008, 157:14-20.
77. Geurden T., Claerebout E., Vercruysse J., Berkvens D. A Bayesian evaluation of four immunological assays for the diagnosis of clinical cryptosporidiosis in calves. Vet J 2008, 176:400-402.
78. Geurden T., Geldhof P., Levecke B., Martens C., Berkvens D., Casaert S., et al. Mixed Giardia duodenalis assemblage A and E infections in calves. Int J Parasitol 2008, 38:259-264.
79. Geurden T., Levecke B., Cacciò S., Visser A., De Groote G., Casaert S., et al. Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium. Parasitology 2009, 136:1161.
80. Geurden T., Levecke B., Pohle H., De Wilde N., Vercruysse J., Claerebout E. A Bayesian evaluation of two dip-stick assays for the on-site diagnosis of infection in calves suspected of clinical giardiasis. Vet Parasitol 2010, 172:337-340.
81. Geurden T., Vercruysse J., Claerebout E. Is Giardia a significant pathogen in production animals?. Exp Parasitol 2010, 124:98-10.
82. Goka A.K., Mathan V.I., Rolston D.D.K., Farthing M.J.G. Diagnosis of giardiasis by specific IgM antibody enzyme-linked immunosorbent assay. Lancet 1986, 328:184-186.
83. Goka A.K.J., Rolston D.D.K., Mathan V.I., Farthing M.J.G. The relative merits of faecal and duodenal juice microscopy in the diagnosis of giardiasis. Trans R Soc Trop Med Hyg 1990, 84:66-67.
84. Goñi P., Martín B., Villacampa M., García A., Seral C., Castillo F.J., et al. Evaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp., Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples. Eur J Clin Microbiol Infect Dis 2012, 31:2077-2082.
85. Green E.L., Miles M.A., Warhurst D.C. Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme-linked immunosorbent assay. Lancet 1985, 326:691-693.
86. Grigoriew G.A., Walmsley S., Law L., Chee S.L., Yang J., Keystone J., et al. Evaluation of the merifluor immunofluorescent assay for the detection of Cryptosporidium and Giardia in sodium acetate formalin-fixed stools. Diagn Microbiol Infect Dis 1994, 19:89-91.
87. Gutiérrez-Cisneros M.J., Martínez-Ruiz R., Subirats M., Merino F.J., Millán R., Fuentes I. Assessment of two commercially available immunochromatographic assays for a rapid diagnosis of Giardia duodenalis and Cryptosporidium spp. in human fecal specimens. Enferm Infecc Microbiol Clin 2011, 29:201-203.
88. Guy R.A., Payment P., Krull U.J., Horgen P.A. Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl Environ Microbiol 2003, 69:5178-5185.
89. Hadrys H., Balick M., Schierwater B. Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol Ecol 1992, 1:55-63.
90. Haque R., Roy S., Kabir M., Stroup S.E., Mondal D., Houpt E.R. Giardia assemblage A infection and diarrhea in Bangladesh. J Infect Dis 2005, 192:2171-2173.
91. Haque R., Roy S., Siddique A., Mondal U., Rahman S.M., Mondal D., et al. Multiplex real-time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. Am J Trop Med Hyg 2007, 76:713-717.
92. Hashimoto T., Nakamura Y., Nakamura F., Shirakura T., Adachi J., Goto N., et al. Protein phylogeny gives a robust estimation for early divergences of eukaryotes: phylogenetic place of a mitochondria-lacking protozoan, Giardia lamblia. Mol Biol Evol 1994, 11:65-71.
93. Higuchi R., Dollinger G., Walsh P.S., Griffith R. Simultaneous amplification and detection of specific DNA sequences. Nat Biotech 1992, 10:413-417.
94. Hill V.R., Polaczyk A.L., Kahler A.M., Cromeans T.L., Hahn D., Amburgey J.E. Comparison of hollow-fiber ultrafiltration to the USEPA VIRADEL technique and USEPA Method 1623. J Environ Qual 2009, 38:822-825.
95. Hopkins R.M., Meloni B.P., Groth D.M., Wetherall J.D., Reynoldson J.A., Thompson R.C.A. Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. J Parasitol 1997, 83:44-51.
96. Hsu B.-M., Huang C. Recovery of Giardia and Cryptosporidium from water by various concentration, elution, and purification techniques. J Environ Qual 2000, 29:1587-1593.
97. Hu J., Feng Y., Ong S.L., Ng W.J., Song L., Tan X., et al. Improvement of recoveries for the determination of protozoa Cryptosporidium and Giardia in water using Method 1623. J Microbiol Meth 2004, 58:321-325.
98. Huang D.B., White A.C. An updated review on Cryptosporidium and Giardia. Gastroenterol Clin North Am 2006, 35:291-314.
99. Hunt C.L., Ionas G., Brown T.J. Prevalence and strain differentiation of Giardia intestinalis in calves in the Manawatu and Waikato regions of North Island, New Zealand. Vet Parasitol 2000, 91:7-13.
100. Jerlstrom-Hultqvist J., Franzen O., Ankarklev J., Xu F., Nohynkova E., Andersson J.O., et al. Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate. BMC Genomics 2010, 11:543.
101. Jex A.R., Stanley K.K., Lo W., Littman R., Verweij J.J., Campbell B.E., et al. Detection of diarrhoeal pathogens in human faeces using an automated, robotic platform. Mol Cell Probes 2012, 26:11-15.
102. Johnston S.P., Ballard M.M., Beach M.J., Causer L., Wilkins P.P. Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens. J Clin Microbiol 2003, 41:623-626.
103. Karanis P., Kourenti C., Smith H. Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt. J Water Health 2007, 5:1-38.
104. Katanik M.T., Schneider S.K., Rosenblatt J.E., Hall G.S., Procop G.W. Evaluation of ColorPAC Giardia/Cryptosporidium rapid assay and ProSpecT Giardia/Cryptosporidium microplate assay for detection of Giardia and Cryptosporidium in fecal specimens. J Clin Microbiol 2001, 39:4523-4525.
105. Kaucner C., Stinear T. Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR. Appl Environ Microbiol 1998, 64:1743-1749.
106. Keserue H.-A., Füchslin H.P., Egli T. Rapid detection and enumeration of Giardia lamblia cysts in water samples by immunomagnetic separation and flow cytometric analysis. Appl Environ Microbiol 2011, 77:5420-5427.
107. Kimble G, Amburgey J, Hilger H. Improvements in Cryptosporidium recovery and variability through modifications to United States Environmental Protection Agency Method 1623. Water Environ J 2012.
108. Knisley C.V., Engelkirk P.G., Pickering L.K., West M.S., Janoff E.N. Rapid detection of Giardia antigen in stool with the use of enzyme immunoassays. Am J Clin Pathol 1989, 91:704-708.
109. Kulda J., Nohynkova E. Flagellates of the human intestine and of intestines of other species. Parasitic protozoa, vol II, intestinal flagellates: histomonads, trichomonads, amoeba, opalinids, and ciliates 1978, 1-138. Academic Press, New York. J. Kreier (Ed.).
110. Kutz SJ, Thompson RCA, Polley L. Wildlife with Giardia: villain, or victim and vector. Giardia and Cryptosporidium: from molecules to disease. Oxford: Oxford University Press; 2009. p. 94-106.
111. Lee G.-C., Nam S.-H., Chae J.-C., Lee C.-H. Giardia duodenalis: improved detection of viable cysts by reverse transcription-PCR of heat shock-inducible hsp 70 gene. Exp Parasitol 2009, 123:377-380.
112. Lemos V., Graczyk T.K., Alves M., Lobo M.L., Sousa M.C., Antunes F., et al. Identification and determination of the viability of Giardia lamblia cysts and Cryptosporidium parvum and Cryptosporidium hominis oocysts in human fecal and water supply samples by fluorescent in situ hybridization (FISH) and monoclonal antibodies. Parasitol Res 2005, 98:48-53.
113. Li W., Zhang N., Gong P., Cao L., Li J., Su L., et al. A novel multiplex PCR coupled with Luminex assay for the simultaneous detection of Cryptosporidium spp., Cryptosporidium parvum and Giardia duodenalis. Vet Parasitol 2010, 173:11-18.
114. Liu J., Gratz J., Amour C., Kibiki G., Becker S., Janaki L., et al. A laboratory-developed TaqMan array card for simultaneous detection of 19 enteropathogens. J Clin Microbiol 2013, 51:472-480.
115. Liu Q., Sommer S.S. Restriction endonuclease fingerprinting (REF): a sensitive method for screening mutations in long, contiguous segments of DNA. BioTechniques 1995, 18:470-477.
116. Lu S.Q., Baruch A.C., Adam R.D. Molecular comparison of Giardia lamblia isolates. Int J Parasitol 1998, 28:1341-1345.
117. MacPherson J.M., Eckstein P.E., Scoles G.J., Gajadhar A.A. Variability of the random amplified polymorphic DNA assay among thermal cyclers, and effects of primer and DNA concentration. Mol Cell Probes 1993, 7:293-299.
118. Mank T.G., Zaat J.O.M., Deelder A.M., van Eijk J.T.M., Polderman A.M. Sensitivity of microscopy versus enzyme immunoassay in the laboratory diagnosis of giardiasis. Eur J Clin Microbiol Infect Dis 1997, 16:615-619.
119. Maraha B., Buiting A.G.M. Evaluation of four enzyme immunoassays for the detection of Giardia lamblia antigen in stool specimens. Eur J Clin Microbiol Infect Dis 2000, 19:485-487.
120. Mason P.R., Patterson B.A. Epidemiology of Giardia lamblia infection in children: cross-sectional and longitudinal studies in urban and rural communities in Zimbabwe. Am J Trop Med Hyg 1987, 37:277-282.
121. McCuin R.M., Clancy J.L. Modifications to United States Environmental Protection Agency Methods 1622 and 1623 for detection of Cryptosporidium oocysts and Giardia cysts in water. Appl Environ Microbiol 2003, 69:267-274.
122. McRoberts K.M., Meloni B.P., Morgan U.M., Marano R., Binz N., Erlandsen S.L., et al. Morphological and molecular characterization of Giardia isolated from the straw-necked ibis (Threskiornis spinicollis) in Western Australia. J Parasitol 1996, 82:711-718.
123. Mekaru S.R., Marks S.L., Felley A.J., Chouicha N., Kass P.H. Comparison of direct immunofluorescence, immunoassays, and fecal flotation for detection of Cryptosporidium spp. and Giardia spp. in naturally exposed cats in 4 Northern California animal shelters. J Vet Intern Med 2007, 21:959-965.
124. Monis P.T., Andrews R.H., Mayrhofer G., Ey P.L. Molecular systematics of the parasitic protozoan Giardia intestinalis. Mol Biol Evol 1999, 16:1135-1144.
125. Monis P.T., Andrews R.H., Mayrhofer G., Ey P.L. Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect Genet Evol 2003, 3:29-38.
126. Monis P.T., Cacciò S.M., Thompson R.C.A. Variation in Giardia: towards a taxonomic revision of the genus. Trends Parasitol 2009, 25:93-100.
127. Monis P.T., Giglio S., Keegan A.R., Thompson R.C.A. Emerging technologies for the detection and genetic characterization of protozoan parasites. Trends Parasitol 2005, 21:340-346.
128. Monis P.T., Mayrhofer G., Andrews R.H., Homan W.L., Limper L., Ey P.L. Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus. Parasitology 1996, 112:1-12.
129. Morgan U.M., Constantine C.C., Greene W.K., Thompson R.C.A. RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data. Trans R Soc Trop Med Hyg 1993, 87:702-705.
130. Morrison H.G., McArthur A.G., Gillin F.D., Aley S.B., Adam R.D., Olsen G.J., et al. Genomic minimalism in the early diverging intestinal parasite Giardia lamblia. Science 2007, 317:1921-1926.
131. Muhsen K., Levine M.M. A systematic review and meta-analysis of the association between Giardia lamblia and endemic pediatric diarrhea in developing countries. Clin Infect Dis 2012, 55:S271-S293.
132. Nantavisai K., Mungthin M., Tan-ariya P., Rangsin R., Naaglor T., Leelayoova S. Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens. J Clin Microbiol 2007, 45:581-583.
133. Nash T.E., Herrington D.A., Levine M.M. Usefulness of an enzyme-linked immunosorbent assay for detection of Giardia antigen in feces. J Clin Microbiol 1987, 25:1169-1171.
134. Nash T.E., Mowatt M.R. Characterization of a Giardia lamblia variant-specific surface protein (VSP) gene from isolate GS/M and estimation of the VSP gene repertoire size. Mol Biochem Parasitol 1992, 51:219-227.
135. Nash T.E., Mowatt M.R. Identification and characterization of a Giardia lamblia group-specific gene. Exp Parasitol 1992, 75:369-378.
136. Ng C.T., Gilchrist C.A., Lane A., Roy S., Haque R., Houpt E.R. Multiplex real-time PCR assay using Scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples. J Clin Microbiol 2005, 43:1256-1260.
137. Nguyen T.K.T., Kherouf H., Blanc-Pattin V., Allais E., Chevalier Y., Richez A., et al. Evaluation of an immunochromatographic assay: Giardia-Strip® (Coris BioConcept) for detection of Giardia intestinalis in human fecal specimens. Eur J Clin Microbiol Infect Dis 2012, 31:623-625.
138. Nolan M.J., Jex A.R., Koehler A.V., Haydon S.R., Stevens M.A., Gasser R.B. Molecular-based investigation of Cryptosporidium and Giardia from animals in water catchments in southeastern Australia. Water Res 2013, 47:1726-1740.
139. Nygård K., Schimmer B., Søbstad Ø., Walde A., Tveit I., Langeland N., et al. A large community outbreak of waterborne giardiasis-delayed detection in a non-endemic urban area. BMC Public Health 2006, 6:1-10.
140. O'Handley R.M., Olson M.E. Giardiasis and cryptosporidiosis in ruminants. Vet Clin North Am - Food A 2006, 22:623.
141. Olson M.E., Goh J., Phillips M., Guselle N., McAllister T.A. Giardia cyst and Cryptosporidium oocyst survival in water, soil, and cattle feces. J Environ Qual 1999, 28:1991-1996.
142. Olson M.E., O'Handley R.M., Ralston B.J., McAllister T.A., Thompson R.C.A. Update on Cryptosporidium and Giardia infections in cattle. Trends Parasitol 2004, 20:185-191.
143. Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci USA 1989, 86:2766-2770.
144. Oster N., Gehrig-Feistel H., Jung H., Kammer J., McLean J.E., Lanzer M. Evaluation of the immunochromatographic CORIS Giardia-Strip test for rapid diagnosis of Giardia lamblia. Eur J Clin Microbiol Infect Dis 2006, 25:112-115.
145. Paintlia A.S., Mahajan R.C., Chakraborti A., Sehgal R., Ganguly N.K. Characterization of Giardia lamblia groups A and B from North India by isoenzyme and random amplified polymorphic DNA analysis. Parasitol Res 1999, 85:510-512.
146. Papini R., Cardini G. Evaluation of a rapid Cryptosporidium/Giardia immunochromatographic test for diagnosis of giardiasis in dogs. Rev Med Vet 2006, 157:490-493.
147. Pelayo L., Fraga J., Núñez F.A., Mendoza D., Torres D.R., Finlay C.M. Genetic characterization by random amplified polymorphic DNA analysis (RAPD) of 18 isolates of Giardia lamblia obtained from day care children. Exp Parasitol 2003, 104:162-166.
148. Perez T., Albornoz J., Dominguez A. An evaluation of RAPD fragment reproducibility and nature. Mol Ecol 1998, 7:1347-1357.
149. Perry D.A., Morrison H.G., Adam R.D. Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate. Mol Biochem Parasitol 2011, 180:112-114.
150. Pillai D.R., Kain K.C. Immunochromatographic strip-based detection of Entamoeba histolytica, E. dispar and Giardia lamblia coproantigen. J Clin Microbiol 1999, 37:3017-3019.
151. Quintero-Betancourt W., Gennaccaro A.L., Scott T.M., Rose J.B. Assessment of methods for detection of infectious Cryptosporidium oocysts and Giardia cysts in reclaimed effluents. Appl Environ Microbiol 2003, 69:5380-5388.
152. Read C.M., Monis P.T., Thompson R.C.A. Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. Infect Genet Evol 2004, 4:125-130.
153. Regnath T., Klemm T., Ignatius R. Rapid and accurate detection of Giardia lamblia and Cryptosporidium spp. antigens in human fecal specimens by new commercially available qualitative immunochromatographic assays. Eur J Clin Microbiol Infect Dis 2006, 25:807-809.
154. Riggs J.L., Dupuis K.W., Nakamura K., Spath D.P. Detection of Giardia lamblia by immunofluorescence. Appl Environ Microbiol 1983, 45:698-700.
155. Rimhanen-Finne R., Enemark H.L., Kolehmainen J., Toropainen P., Hänninen M.L. Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs. Vet Parasitol 2007, 145:345-348.
156. Robertson L.J., Forberg T., Hermansen L., Hamnes I.S., Gjerde B. Giardia duodenalis cysts isolated from wild moose and reindeer in Norway: genetic characterization by PCR-RFLP and sequence analysis at two genes. J Wildl Dis 2007, 43:576-585.
157. Rocha M.O., Gomes M.A., Costa A.O., Furst C., Silva E.F. Molecular characterization of Brazilian human Giardia duodenalis isolates using isoenzyme and random amplified polymorphic DNA analysis. Diagn Microbiol Infect Dis 2003, 46:273-278.
158. Rosenblatt J.E., Sloan L.M., Schneider S.K. Evaluation of an enzyme-linked immunosorbent assay for the detection of Giardia lamblia in stool specimens. Diagn Microbiol Infect Dis 1993, 16:337-341.
159. Rosoff J.D., Sanders C.A., Sonnad S.S., De Lay P.R., Hadley W.K., Vincenzi F.F., et al. Stool diagnosis of giardiasis using a commercially available enzyme immunoassay to detect Giardia-specific antigen 65 (GSA 65). J Clin Microbiol 1989, 27:1997-2002.
160. Ross A.G., Olds G.R., Cripps A.W., Farrar J.J., McManus D.P. Enteropathogens and chronic illness in returning travelers. New Engl J Med 2013, 368:1817-1825.
161. Ryan U.M., Cacciò S.M. Zoonotic potential of Giardia. Int J Parasitol 2013, 43:943-956.
162. Sauch J.F. Use of immunofluorescence and phase-contrast microscopy for detection and identification of Giardia cysts in water samples. Appl Environ Microbiol 1985, 50:1434-1438.
163. Sauch J.F., Flanigan D., Galvin M.L., Berman D., Jakubowski W. Propidium iodide as an indicator of Giardia cyst viability. Appl Environ Microbiol 1991, 57:3243-3247.
164. Savioli L., Smith H.V., Thompson R.C.A. Giardia and Cryptosporidium join the 'Neglected Diseases Initiative'. Trends Parasitol 2006, 22:203-208.
165. Scheffler E.H., Van Etta L.L. Evaluation of rapid commercial enzyme immunoassay for detection of Giardia lamblia in formalin-preserved stool specimens. J Clin Microbiol 1994, 32:1807-1808.
166. Schunk M., Jelinek T., Wetzel K., Nothdurft H.D. Detection of Giardia lamblia and Entamoeba histolytica in stool samples by two enzyme immunoassays. Eur J Clin Microbiol Infect Dis 2001, 20:389-391.
167. Schupp D.G., Erlandsen S.L. A new method to determine Giardia cyst viability: correlation of fluorescein diacetate and propidium iodide staining with animal infectivity. Appl Environ Microbiol 1987, 53:704-707.
168. Schupp D.G., Januschka M.M., Sherlock L.A., Stibbs H.H., Meyer E.A., Bemrick W.J., et al. Production of viable Giardia cysts in vitro: determination by fluorogenic dye staining, excystation, and animal infectivity in the mouse and Mongolian gerbil. Gastroenterology 1988, 95:1-10.
169. Schuurman T., Lankamp P., Van Belkum A., Kooistra-Smid M., Van Zwet A. Comparison of microscopy, real-time PCR and a rapid immunoassay for the detection of Giardia lamblia in human stool specimens. Clin Microbiol Infect 2007, 13:1186-1191.
170. Šedinová J., Flegr J., Ey P.L., Kulda J. Use of random amplified polymorphic DNA (RAPD) analysis for the identification of Giardia intestinalis subtypes and phylogenetic tree construction. J Euk Microbiol 2003, 50:198-203.
171. Smith A.L., Smith H.V. A comparison of fluorescein diacetate and propidium iodide staining and in vitro excystation for determining Giardia intestinalis cyst viability. Parasitology 1989, 99:329-331.
172. Smith H.V., Paget T. Giardia. Infectious disease: foodborne diseases 2007, 303-336. Humana Press, Totowa, New Jersey. S. Simjee (Ed.).
173. Sogin M.L., Gunderson J.H., Elwood H.J., Alonso R.A., Peattie D.A. Phylogenetic meaning of the kingdom concept: an unusual ribosomal RNA from Giardia lamblia. Science 1989, 243:75.
174. Sousa M.C., Morais J.B., Machado J.E., Poiares-da-Silva J. Genotyping of Giardia lamblia human isolates from Portugal by PCR-RFLP and sequencing. J Euk Microbiol 2006, 53:S174-S176.
175. Stark D., Al-Qassab S.E., Barratt J.L.N., Stanley K., Roberts T., Marriott D., et al. Evaluation of multiplex tandem real-time PCR for detection of Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in clinical stool samples. J Clin Microbiol 2011, 49:257-262.
176. Stark D., Barratt J.L., van Hal S., Marriott D., Harkness J., Ellis J.T. Clinical significance of enteric protozoa in the immunosuppressed human population. Clin Microbiol Rev 2009, 22:634-650.
177. Stibbs H.H., Samadpour M., Manning J.F. Enzyme immunoassay for detection of Giardia lamblia cyst antigens in formalin-fixed and unfixed human stool. J Clin Microbiol 1988, 26:1665-1669.
178. Strand E., Robertson L., Hanevik K., Alvsvåg J., Mørch K., Langeland N. Sensitivity of a Giardia antigen test in persistent giardiasis following an extensive outbreak. Clin Microbiol Infect 2008, 14:1069-1071.
179. Sulaiman I.M., Fayer R., Bern C., Gilman R.H., Trout J.M., Schantz P.M., et al. Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis. Emerg Infect Dis 2003, 9:1444-1452.
180. Sulaiman I.M., Jiang J., Singh A., Xiao L. Distribution of Giardia duodenalis genotypes and subgenotypes in raw urban wastewater in Milwaukee, Wisconsin. Appl Environ Microbiol 2004, 70:3776-3780.
181. Taniuchi M., Verweij J.J., Noor Z., Sobuz S.U., Lieshout L., Petri W.A., et al. High throughput multiplex PCR and probe-based detection with Luminex beads for seven intestinal parasites. Am J Trop Med Hyg 2011, 84:332-337.
182. Thiriat L., Sidaner F., Schwartzbrod J. Determination of Giardia cyst viability in environmental and faecal samples by immunofluorescence, fluorogenic dye staining and differential interference contrast microscopy. Lett Appl Microbiol 1998, 26:237-242.
183. Thompson S. Giardia lamblia in children and the child care setting: a review of the literature. J Paediatr Child Health 1994, 30:202-209.
184. Thompson R.C.A. The zoonotic significance and molecular epidemiology of Giardia and giardiasis. Vet Parasitol 2004, 126:15-35.
185. Thompson R.C.A., Constantine C.C., Morgan U.M. Overview and significance of molecular methods: what role for molecular epidemiology?. Parasitology 1998, 117:S161-S175.
186. Thompson R.C.A., Monis P.T. Variation in Giardia: implications for taxonomy and epidemiology. Adv Parasitol 2004, 58:69-137.
187. Thompson R.C.A., Monis P.T. Taxonomy of Giardia species. Giardia 2011, 3-15. Springer, Vienna. H.D. Lujan, S. Svärd (Eds.).
188. Thompson R.C.A., Monis P.T. Giardia - from genome to proteome. Adv Parasitol 2012, 78:57-95.
189. Thornton S.A., West A.H., DuPont H.L., Pickering L.K. Comparison of methods for identification of Giardia lamblia. Am J Clin Pathol 1983, 80:858-860.
190. Ungar B.L.P., Yolken R.H., Nash T.E., Quinn T.C. Enzyme-linked immunosorbent assay for the detection of Giardia lamblia in fecal specimens. J Infect Dis 1984, 149:90-97.
191. Upcroft J.A., Krauer K.G., Upcroft P. Chromosome sequence maps of the Giardia lamblia assemblage A isolate WB. Trends Parasitol 2010, 26:484-491.
192. US-EPA. National primary drinking regulation: monitoring requirements for public drinking water supplies: Cryptosporidium, Giardia, viruses, disinfection byproducts, water treatment plant data and other information requirements. 1996.
193. US-EPA. Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. 1999.
194. US-EPA. Method 1623.1: Cryptosporidium and Giardia in water by filtration/IMS/FA, 2012.
195. van Belkum A., Homan W., Limper L., Quint W.G.V. Genotyping isolates and clones of Giardia duodenalis by polymerase chain reaction: implications for the detection of genetic variation among protozoan parasite species. Mol Biochem Parasitol 1993, 61:69-77.
196. Van Kerkhoven D., Lontie M., Verhaegen J., Lagrou K. Evaluation of the Giardia-strip: an in vitro immunochromatographic test for the detection of Giardia lamblia cysts in faecal specimens. Clin Microbiol Infect 2006, 12:1.
197. van Keulen H., Homan W.L., Erlandsen S.L., Jarroll E.L. A three nucleotide signature sequence in small subunit rRNA divides human Giardia in two different genotypes. J Euk Microbiol 1995, 42:392-394.
198. van Keulen H., Macechko P.T., Wade S., Schaaf S., Wallis P.M., Erlandsen S.L. Presence of human Giardia in domestic, farm and wild animals, and environmental samples suggests a zoonotic potential for giardiasis. Vet Parasitol 2002, 108:97-107.
199. van Zanten E., Wisselink G.J., Stoll S., Alvarez R., Kooistra-Smid A.M.D. Evaluation of a shortened QIAsymphony DNA extraction protocol for stool samples using a multiplex real-time PCR for the detection of enteric pathogens. J Microbiol Meth 2011, 84:365-367.
200. Vesey G., Hutton P., Champion A., Ashbolt N., Williams K.L., Warton A., et al. Application of flow cytometric methods for the routine detection of Cryptosporidium and Giardia in water. Cytometry 1994, 16:1-6.
201. Wallner G., Amann R., Beisker W. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 1993, 14:136-143.
202. Warren W., Hovig E., Smith-Sørensen B., Børresen A.L., Fujimura F.K., Liu Q., et al. Detection of mutations by single-strand conformation polymorphism (SSCP) analysis and SSCP-hybrid methods. Curr Protoc Human Genetics 1997, 7(4):1-23.
203. Weiss J.B., van Keulen H., Nash T.E. Classification of subgroups of Giardia lamblia based upon ribosomal RNA gene sequence using the polymerase chain reaction. Mol Biochem Parasitol 1992, 54:73-86.
204. Weitzel T., Dittrich S., Möhl I., Adusu E., Jelinek T. Evaluation of seven commercial antigen detection tests for Giardia and Cryptosporidium in stool samples. Clin Microbiol Infect 2006, 12:656-659.
205. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990, 18:7213-7218.
206. WHO. Guidelines for drinking-water quality. Fourth edition. Geneva: World Health Organization; 2011a.
207. WHO. Microbial fact sheets. Guidelines for drinking-water quality, second edition. Geneva: World Health Organization; 2011b. p. 231-306.
208. Wielinga C.M., Thompson R.C.A. Comparative evaluation of Giardia duodenalis sequence data. Parasitology 2007, 134:1795-1821.
209. Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A., Tingey S.V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990, 18:6531-6535.
210. Wolfe M.S. Clinical symptoms and diagnosis by traditional methods. Giardiasis 1990, 175-185. Elsevier, New York. E.A. Meyer (Ed.).
211. Xiao L., Fayer R. Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission. Int J Parasitol 2008, 38:1239-1255.
212. Yee J., Dennis P.P. Isolation and characterization of a NADP-dependent glutamate dehydrogenase gene from the primitive eucaryote Giardia lamblia. J Bio Chem 1992, 267:7539-7544.
213. Yolken R., Ungar B. ELISA detection of Giardia lamblia. The Lancet 1985, 326:1120.
214. Yong T., Han K., Yang H., Park S. PCR-RFLP analysis of Giardia intestinalis using a Giardia-specific gene, GLORF-C4. Parasite 2002, 9:65-70.
215. Yu X., Van Dyke M.I., Portt A., Huck P.M. Development of a direct DNA extraction protocol for real-time PCR detection of Giardia lamblia from surface water. Ecotoxicology 2009, 18:661-668.
216. Zarlenga D.S., Trout J.M. Concentrating, purifying and detecting waterborne parasites. Vet Parasitol 2004, 126:195-217.
217. Zhang P, Liu Y, Alsarakibi M, Li J, Liu T, Li Y, et al. Application of HRM assays with EvaGreen dye for genotyping Giardia duodenalis zoonotic assemblages. Parasitol Res 2012:1-7.
218. Zimmerman S.K., Needham C.A. Comparison of conventional stool concentration and preserved-smear methods with Merifluor Cryptosporidium/Giardia direct immunofluorescence assay and ProSpecT Giardia EZ microplate assay for detection of Giardia lamblia. J Clin Microbiol 1995, 33:1942-1943.
219. Zuckerman U., Tzipori S. Portable continuous flow centrifugation and Method 1623 for monitoring of waterborne protozoa from large volumes of various water matrices. J Appl Microbiol 2006, 100:1220-1227.