Evaluation of a shortened QIAsymphony DNA extraction protocol for stool samples using a multiplex real-time PCR for the detection of enteric pathogens

Journal of Microbiological Methods

Volumn 84, Issue 2, 2011, Pages 365-367

  van Zanten, E ^a   Wisselink, G J ^a   Stoll, S ^b   Alvarez, R ^b   Kooistra Smid, A M D ^a  

^a Department of Research and Development ^*  (Netherlands)

^b QIAGEN GMBH   (Germany)

Author keywords

DNA extraction; Enteric pathogens; Evaluation; QIAsymphony; Real time PCR

Indexed keywords

ARTICLE; CAMPYLOBACTER JEJUNI; CONTROLLED STUDY; CRYPTOSPORIDIUM; DIENTAMOEBA; DNA EXTRACTION; ENTAMOEBA HISTOLYTICA; FECES ANALYSIS; GIARDIA LAMBLIA; MICROORGANISM DETECTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SALMONELLA ENTERICA; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SHIGELLA;

BACTERIOLOGICAL TECHNIQUES; DNA; ENTEROBACTERIACEAE; FECES; HUMANS; TIME FACTORS;

EID: 79251602149     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.12.023     Document Type: Article

References (2)

1. de Boer, R.F., Ott, A., Kesztyus, B., Kooistra-Smid, A.M., 2010. Improved detection of five major gastrointestinal pathogens by use of a molecular screening approach. J. Clin. Microbiol. 48, 4140-4146.
2. van Zanten, E., Wisselink, G.J., de Boer, W., Stoll, S., Alvarez, R., Kooistra-Smid, A.M.D., 2010. Comparison of the QIAsymphony automated Nucleic Acid Extraction and PCR setup platforms with NucliSens easyMAG and Corbett CAS-1200 liquid handling station for the detection of enteric pathogens in fecal samples. (Electronic publication ahead of print).