Analysis-only Detection of Giardia by Combining Immunomagnetic Separation and Two-Color Flow Cytometry

Cytometry Part A

Volumn 51, Issue 2, 2003, Pages 79-86

  Ferrari, Belinda C ^a   Veal, Duncan ^a  

^a MACQUARIE UNIVERSITY   (Australia)

Author keywords

Flow cytometry; Fluorescent in situ hybridization; Giardia; Immunomagnetic separation; Monoclonal antibody; Peptide nucleic acid; Two color

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE; MONOCLONAL ANTIBODY; PEPTIDE NUCLEIC ACID; PHYCOERYTHRIN;

ARTICLE; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; GIARDIA; IMMUNOMAGNETIC SEPARATION; NONHUMAN; PRIORITY JOURNAL; PROTOZOON; RELIABILITY; WATER ANALYSIS;

ANIMALS; ANTIBODIES, MONOCLONAL; FLOW CYTOMETRY; FLUORESCENT DYES; GIARDIA LAMBLIA; IMMUNOMAGNETIC SEPARATION; IN SITU HYBRIDIZATION, FLUORESCENCE; REPRODUCIBILITY OF RESULTS;

EID: 0348135033     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Adam DA. The biology of Giardia spp. Microbiol Rev 1991;55:706-732.
2. O'Donoghue PJ. Cryptosporidium and cryptosporidiosis in man and animals. Int J Parasitol 1995;25:1-55.
3. Water supply (water quality) (amendment) regulations SI No 1524: Cryptosporidium in water supplies. London: Drinking Water Inspectorate, Department of the Environment, Transport and the Regions; 1999.
4. Campbell AT, Gron B, Johnson SE. Immunomagnetic separation of Cryptosporidium oocysts from high turbidity water sample concentrates. In: Fricker CR, Clancy JL, Rochelle PA, editors. International Symposium on Waterborne Cryptosporidium. Denver, CO: American Water Works Association; 1997. p 91-96.
5. Bukhari Z, McCuin RM, Fricker CR, Clancy JL. Immunomagnetic separation of Cryptosporidium parvum from source water samples of various turbidities. Appl Environ Microbiol 1998;64:4495-4499.
6. Arrowood MJ and Sterling CR. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J Parasitol 1987;73:314.
7. Vesey G, Slade JS, Fricker CR. Taking the eye strain out of environmental Cryptosporidium analysis. Lett Appl Microbiol 1991;13:62-65.
8. Vesey G, Hutton P, Champion A, Ashbolt N, Williams KL, Warton A, Veal DA, Application of flow cytometric methods for the routine detection of Cryptosporidium and Giardia in water. Cytometry 1994;16:1-6.
9. Hoffman RM, Standridge JH, Prieve AF, Cununato JC, Bernhardt M. Using flow cytometry to detect protozoa. J Am Water Works Assoc 1997;89:104-111.
10. Parks DR, Hardy RR, Herzenberg LA. Three colour immunofluorescence analysis of mouse B-lymphocyte sub-populations. Cytometry 1984;5:159-168.
11. Festin R, Bjorklund B, Totterman TH. Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry with colloidal gold, fluorescein and phycoerythrin as labels. J Immunol Methods 1987;101:23-28.
12. Shapiro HM. Practical flow cytometry. 3rd ed. New York: Alan R. Liss; 1995.
13. Moore AG, Vesey G, Champion A, Scandizzo P, Deere D, Veal DA, Williams KL. Viable Cryptosporidium parvum oocysts exposed to chlorine or other oxidising conditions may lack identifying epitopes. Int J Parasitol 1998;28:1205-1212.
14. Weir C, Vesey G, Slade M, Ferrari B, Williams K, Veal DA. An immunoglobulin G1 monoclonal antibody highly specific to the wall of Cryptosporidium oocysts. Clin Diagn Lab Immunol 2000;7:745-750.
15. Ferrari BC, Vesey G, Davis KA, Gauci M, Veal D. A novel two-color flow cytometric assay for the detection of Cryptosporidium in environmental water samples. Cytometry 2000;41:216-222.
16. Vesey G, Deere D, Gauci MR, Griffiths KR, Williams KL, Veal DA. Evaluation of fluorochromes for immunofluorescent labeling of microorganisms in environmental water samples. Cytometry 1997;29:147-154.
17. Rochelle PA, De Leon R, Johnson A, Stewart MH, Wolfe RL. Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples. Appl Environ Microbiol 1999;65:841-845.
18. Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. Washington, DC: US Environment Protection Agency; 1999.
19. Method 1622: Cryptosporidium in water by Filtration/IMS/FA. Washington, DC: US Environmental Protection Agency; 1999. p EPA 821-R-97-021.
20. Campbell A, Smith H. Immunomagnetic separation of Cryptosporidium oocysts from water samples: round robin comparison of techniques. Water Sci Technol 1997;35:397-401.
21. Veal DA, Scandizzo P, Dorsch M. Detection of Cryptosporidium and Giardia in biosolids. Sydney: Department of Biological Sciences, Macquarie University; 2000. p 27.
22. Ferrari BC. The development of dual-colour flow cytometric analysis of Cryptosporidium and Giardia in water. Division of Environmental and life sciences. Sydney: Macquarie University; 2000. p 280.
23. Veal DA, Dorsch MR, Ferrari BC. Automated detection and viability assessment. In: Smith M, Thompson KC, editors. Cryptosporidium: the analytical challenge. Cambridge: Royal Society of Chemistry; 2001. p 143-154.
24. Vesey G, Ashbolt N, Fricker E. The use of ribosomal rRNA targeted oligonucleotide probes for fluorescent labelling viable Cryptosporidium oocysts. J Appl Environ Microbiol 1998;85:429-440.
25. Deere D, Vesey G, Ashbolt N, Davies KA, Williams KL, Veal D. Evaluation of fluorochromes for flow cytometric detection of Cryptosporidium parvum oocysts labelled by fluorescent in situ hybridization. Lett Appl Microbiol 1998;27:352-356.
26. Prescott AM, Fricker CR. In situ reverse transcription for the specific detection of bacteria and protozoa. Lett Appl Microbiol 1999;29:396-400.
27. Wallner G, Steinmetz I, Bitter-Suermann D, Amann R. Combination of rRNA-targeted probes and immuno-probes for the identification of bacteria by flow cytometry. System Appl Microbiol 1996;19:569-576.
28. Nielsen PE, Egholm M, Berg RH, Buchardt O. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science 1991;254:1497-1500.
29. Just T, Burgwald H, Broe MK. Flow cytometric detection of EBV (EBER snRNA) using peptide nucleic acid probes. J Virol Methods 1998;73:163-174.
30. Vesey G, Deere D, Weir CJ, Ashbolt N, Williams KL, Veal DA. A simple method for evaluating Cryptosporidium-specific antibodies used in monitoring environmental water samples. Lett Appl Microbiol 1997;25:316-320.
31. Vesey G. Application of flow cytometry to the detection of Cryptosporidium in water. Sydney: School of Biological Sciences, Macquarie University; 1996.
32. Deere D, Vesey G, Milner M, Williams K, Ashbolt N, Veal D. Rapid method for fluorescent in situ ribosomal RNA labelling of Cryptosporidium parvum. J Appl Microbiol 1998;85:807-818.