LNA probes in a real-time TaqMan PCR assay for genotyping of Giardia duodenalis in wastewaters

Journal of Applied Microbiology

Volumn 108, Issue 5, 2010, Pages 1594-1601

  Alonso, J L ^a   Amoros I ^a   Cuesta, G ^a  

^a UNIVERSITAT POLITÈCNICA DE VALÈNCIA   (Spain)

Author keywords

Assemblages; Giardia duodenalis; LNA probe; QPCR; Wastewater; giardin

Indexed keywords

ASSAYS; GENES; POLYMERASE CHAIN REACTION; WASTEWATER TREATMENT;

ASSEMBLAGE; GENOTYPING; GIARDIA DUODENALIS; IMMUNOFLUORESCENCE ASSAY; LOCKED NUCLEIC ACID; LOCKED NUCLEIC ACID PROBE; QPCR; WASTE WATER TREATMENT PLANTS; WASTEWATER SAMPLES; Β-GIARDIN;

PROBES;

LOCKED NUCLEIC ACID;

ABUNDANCE; BIOASSAY; CYST; DETECTION METHOD; DNA; GENETIC ANALYSIS; GENOTYPE; IMMUNOASSAY; METHODOLOGY; MICROORGANISM; POLYMERASE CHAIN REACTION; REAL TIME; SPATIAL DISTRIBUTION; WASTEWATER;

ARTICLE; CONTROLLED STUDY; CYST (RESTING STAGE); GENE AMPLIFICATION; GENOTYPE; GIARDIA DUODENALIS; IMMUNOFLUORESCENCE TEST; NONHUMAN; NUCLEIC ACID PROBE; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PARASITE ISOLATION; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SPAIN; SPECIES COMPARISON; WASTE WATER TREATMENT PLANT; WATER ANALYSIS;

GIARDIA; GIARDIA INTESTINALIS;

EID: 77950681547     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04559.x     Document Type: Article

References (25)

1. Anceno, A.J., Katayama, H., Houpt, E.R., Chavalitshewinkoon-Petmitr, P., Chuluun, B. Shipin, O.V. (2007) IMS-free DNA extraction for the PCR-based quantification of Cryptosporidium and Giardia lamblia in surface and wastewater. Int J Environ Health Res 17, 297 310.
2. Bertrand, I. Schwartzbrod, J. (2007) Detection and genotyping of Giardia duodenalis in wastewater: relation between assemblages and faecal contamination origin. Wat Res 41, 3675 3682.
3. Cacciò, S.M., De Giacomo, M. Pozio, E. (2002) Sequence analysis of the β-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int J Parasitol 32, 1023 1030.
4. Cacciò, S.M., De Giacomo, M., Aulicino, F.A. Pozio, E. (2003) Giardia cysts in wastewater treatment plants in Italy. Appl Environ Microbiol 69, 3393 3398.
5. Cacciò, S.M., Thompson, R., McLauchlin, J. Smith, H. (2005) Untravelling Cryptosporidium and Giardia epidemiology. Trends Parasitol 21, 430 437.
6. Castro-Hermida, J.A., García-Presedo, I., Almeida, A., González-Warleta, M., Correia Da Costa, J.M. Mezo, M. (2008) Contribution of treated wastewater to the contamination of recreational rivera areas with Cryptosporidium spp. and Giardia duodenalis. Wat Res 42, 3528 3538.
7. Gelanew, T., Lalle, M., Hailu, A., Pozio, E. Cacciò, S.M. (2007) Molecular characterization of human isolates of Giardia duodenalis from Ethiopia. Acta Trop 102, 92 99.
8. van der Giessen, J.W., de Vries, A., Roos, M., Wielinga, P., Kortbeek, L.M. Mank, T.G. (2006) Genotyping of Giardia in Dutch patients and animals: a phylogenetic analysis oh human and animal isolates. Int J Parasitol 36, 849 858.
9. Giovanni, G.D., Betancourt, W.Q., Hernandez, J., Assadian, N.W., Flores, J.P. Jaramillo, E.J. (2006) Investigation of potential zooanthroponotic transmission of cryptosporidiosis and giardiasis through agricultural use of reclaimed wastewater. Int J Environ Health Res 16, 405 418.
10. Guy, R.A., Payment, P., Krull, U.J. Horgen, P.A. (2003) Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl Environ Microbiol 69, 5178 5185.
11. Guy, R.A., Xiao, C. Horgen, P.A. (2004) Real-time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens. J Clin Microbiol 42, 3317 3320.
12. Jiang, J., Alderisio, K.A., Singh, A. Xiao, L. (2005) Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates for relief of PCR inhibitors. Appl Environ Microbiol 71, 1135 1141.
13. Kumar, R., Singh, S.K., Koshkin, A.A., Rajwanshi, V.K., Meldgaard, M. Wengel, J. (1998) The first analogues of LNA (locked nucleic acids): phosphorohioate-LNA and 2′thio-LNA. Bioorg Med Chem Lett 8, 2219 2222.
14. Lalle, M., Pozio, E., Capelli, G., Bruschi, F., Crotti, D. Cacciò, S.M. (2005a) Genetic heterogeneity at the β-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes. Int J Parasitol 35, 207 213.
15. Lalle, M., Jimenez-Cardosa, E., Cacciò, S.M. Pozio, E. (2005b) Genotyping of Giardia duodenalis from humans and dogs from Mexico using a β-giardin nested polymerase chain reaction assay. J Parasitol 91, 203 205.
16. Monis, P.T. Thompson, R.C.A. (2003) Cryptosporidium and Giardia-zoonoses: fact or fiction? Infect Genet Evol 3, 233 244.
17. Monis, P.T., Andrews, R.H., Mayrhofer, G. Ey, P.L. (2003) Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect Genetic Evol 3, 29 38.
18. Nichols, R.A., Campbell, B.M. Smith, H.V. (2003) Identification of Cryptosporidium spp. oocysts in United Kingdom noncarbonated natural mineral waters and drinking waters bu using a modified nested PCR-restriction fragment length polymorphism assay. Appl Environ Microbiol 69, 4183 4189.
19. Nolan, T., Hands, R.E. Bustin, S.A. (2006) Quantification of mRNA using real-time RT-PCR. Nature Protocols 1, 1559 1582.
20. Reynisson, E., Josefsen, M.H., Krause, M. Hoorfar, J. (2006) Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR. J Microbiol Methods 66, 206 216.
21. Robertson, L.J., Hermansen, L. Gjerde, B.K. (2006) Occurrence of Cryptosporidium oocysts and Giardia cysts in sewage in Norway. Appl Environ Microbiol 72, 5297 5303. (Pubitemid 44255343)
22. Robertson, L.J., Forberg, T. Gjerde, B.K. (2008) Giardia cysts in sewage influent in Bergen, Norway 15-23 months after an extensive waterborne outbreak of giardiasis. J. Appl Microbiol 104, 1147 1152. (Pubitemid 351393056)
23. Smith, S., Vigilant, L. Morin, P.A. (2002) The effects of sequence length and oligonucleotide mismatches on 5′ exonuclease assay efficiency. Nucleic Acids Res 30, 111.
24. Sulaiman, I.M., Jiang, J., Singh, A. Xiao, L. (2004) Distribution of Giardia duodenalis genotypes and subgenotypes in raw urban wastewater in Milwaukee, Wisconsin. Appl Environ Microbiol 70, 3776 3780. (Pubitemid 38745935)
25. Wilhelm, J., Hahn, M. Pingoud, A. (2000) Influence of DNA target melting behaviour on real-time PCR quantification. Clin Chem 46, 1738 1743.