Optimization of a flow cytometry protocol for detection and viability assessment of Giardia lamblia

Travel Medicine and Infectious Disease

Volumn 6, Issue 4, 2008, Pages 234-239

  Barbosa, Joana ^a,b   Costa de Oliveira, Sofia ^a   Rodrigues, Acácio Gonçalves ^a,c   Pina Vaz, Cidália ^a,c  

^a UNIVERSITY OF PORTO   (Portugal)

^b INSTITUTO PIAGET   (Portugal)

^c HOSPITAL DE SÃO JOÃO   (Portugal)

Author keywords

Monoclonal antibodies; Protozoan parasite; Water analysis

Indexed keywords

FLUORESCEIN; MONOCLONAL ANTIBODY; PROPIDIUM IODIDE;

ARTICLE; CANDIDA ALBICANS; CONTROLLED STUDY; CROSS REACTION; CRYPTOSPORIDIUM PARVUM; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; GIARDIA LAMBLIA; NONHUMAN; OOCYST; PARASITE IDENTIFICATION; PARASITE VIABILITY; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, PROTOZOAN; FLOW CYTOMETRY; FLUORESCEIN; GIARDIA LAMBLIA; HUMANS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; WATER;

EID: 45049083532     PISSN: 14778939     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tmaid.2008.01.004     Document Type: Article

References (22)

1. Marshall M.M., Naumovitz D., Ortega Y., and Sterling C.R. Waterborne protozoan pathogens. Clin Microb Rev 10 (1997) 67-85
2. World Health Organization. Guidelines for drinking-water quality, 3rd ed. 2004.
3. Thompson R.C., Reynoldson J.A., and Mendis A.H. Giardia and giardiasis. Adv Parasitol 32 (1993) 71-160
4. Boreham P.F., Upcroft J.A., and Upcroft P. Changing approaches to the study of Giardia epidemiology: 1681-2000. Int J Parasitol 20 (1990) 479-487
5. Weitzel T., Dittrich S., Mohl I., Adusu E., and Jelinek T. Evaluation of seven commercial antigen detection tests for Giardia and Cryptosporidium in stool samples. Eur J Clin Microbiol Infect Dis 12 (2006) 655-659
6. Verweij J., Blange R.A., Templeton K., Schinkel J., Brienen E.A., van Rooyen M.A., et al. Simultaneous detection of Entamoeba histolytica, Giardia lamblia and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J Clin Microbiol 42 (2004) 1220-1223
7. Dixon B.R., Parenteau M., Martineau C., and Fournier J. A comparison of conventional microscopy, immunofluorescence microscopy and flow cytometry in the detection of Giardia lamblia cysts in beaver fecal samples. J Immunol Methods 202 (1997) 27-33
8. Ghosh S., Debnath A., Sil A., De S., Chattopadhyay D.J., et al. PCR detection of Giardia lamblia in stool: targeting intergenic spacer region of multicopy rRNA gene. Mol Cell Probes 14 (2000) 181-189
9. Hsu B.M., Wu N.M., Jang H.D., Shih F.C., Wan M.T., and Kung C.M. Using the flow cytometry to quantify the Giardia cysts and Cryptosporidium oocysts in water samples. Environ Monit Assess 104 (2005) 155-162
10. Ferrari B.C., Stoner K., and Bergquist P.L. Applying fluorescence based technology to the recovery and isolation of Cryptosporidium and Giardia from industrial wastewater streams. Water Res 40 (2006) 541-548
11. Pina-Vaz C., Costa-Oliveira S., Rodrigues A.G., and Salvador A. Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples. J Clin Microbiol 42 (2004) 906-908
12. Pina-Vaz C., Costa-Oliveira S., and Rodrigues A.G. Safe susceptibility testing of Mycobacterium tuberculosis by flow cytometry with the fluorescent nucleic acid stain SYTO 16. J Med Microbiol 53 (2004) 77-81
13. Pina-Vaz C., Sansonetty F., Rodrigues A.G., Costa-de-Oliveira S., Tavares C., et al. Cytometric approach for a rapid evaluation of susceptibility of Candida strains to antifungals. Clin Microbiol Infect 7 (2001) 609-618
14. Pina-Vaz C., Costa-Oliveira S., Rodrigues A.G., and Espinel-Ingroff A. Comparison of two probes for testing susceptibilities of pathogenic yeasts to voriconazole, itraconazole, and caspofungin by flow cytometry. J Clin Microbiol 43 (2005) 4674-4679
15. Pina-Vaz C., Rodrigues A., Sansonetty F., Martinez-de-Olievira J., Fonseca A.P., and Mardh P.-A. Antifungal activity of local anesthetics against Candida species. Inf Dis Obst Gynecol 8 (2000) 124-137
16. Pina-Vaz C., Sansonetty F., Rodrigues A., Martinez-de-Olievira J., Fonseca A.P., and Mardh P.-A. Antifungal activity of ibuprofen alone and in combination with fluconazol against Candida species. J Med Microbiol 49 (2000) 831-840
17. Barbosa J., Costa-de-Oliveira S., Rodrigues A.G., Hanscheid T., Shapiro H., and Pina-Vaz C. A flow cytometric protocol for detection of Cryptosporidium spp. Cytometry A 73 (2008) 44-47
18. de Roubin M.R., Pharamond J.S., Zanelli F., Poty F., Houdart S., Laurent F., et al. Application of laser scanning cytometry followed by epifluorescent and differential interference contrast microscopy for the detection and enumeration of Cryptosporidium and Giardia in raw and potable waters. J Appl Microbiol 93 (2002) 507-599
19. Lemos V., Graczyk T.K., Alves M., Lobo M.L., Sousa M.C., Antunes F., et al. Identification and determination of the viability of Giardia lamblia cysts and Cryptosporidium parvum and Cryptosporidium hominis oocysts in human fecal and water supply samples by fluorescent in situ hybridization (FISH) and monoclonal antibodies. Parasitol Res 98 (2005) 48-53
20. Haugland R.P. Handbook of fluorescence probes. 9th ed. (1996), Eugene Molecular probes Inc
21. McGuigan K.G., Méndez-Hermida F., Castro-Hermida J.S., Ares-Mazás E., Kehoe S.C., et al. Batch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water. J Appl Microbiol 101 (2006) 453-463
22. Hayes S.L., Rice E.W., Ware M.W., and Schaefer III F.W. Low pressure ultraviolet studies for inactivation of Giardia muris cysts. J Appl Microbiol 94 (2003) 54-59