IMS-free DNA extraction for the PCR-based quantification of Cryptosporidium parvum and Giardia lamblia in surface and waste water

International Journal of Environmental Health Research

Volumn 17, Issue 4, 2007, Pages 297-310

  Anceno, Alfredo J ^a   Katayama, Hiroyuki ^b   Houpt, Eric R ^c   Chavalitshewinkoon Petmitr, Porntip ^d   Chuluun, Buyan ^a   Shipin, Oleg V ^a  

^a ASIAN INSTITUTE OF TECHNOLOGY   (Thailand)

^b UNIVERSITY OF TOKYO   (Japan)

^c UNIVERSITY OF VIRGINIA   (United States)

^d MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Cryptosporidium; Giardia; Pathogen removal; PCR inhibitors; Real time PCR

Indexed keywords

CHELATING AGENT; RESIN; SURFACE WATER; THIOCYANIC ACID DERIVATIVE;

DNA; LYSIS; PATHOGEN; POLYMERASE CHAIN REACTION; PROTOZOAN; QUANTITATIVE ANALYSIS; SURFACE WATER; WASTEWATER;

ARTICLE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CRYPTOSPORIDIUM PARVUM; DNA EXTRACTION; GIARDIA LAMBLIA; IMMUNOMAGNETIC SEPARATION; LYSIS; MICROORGANISM DETECTION; NONHUMAN; OOCYST; PHYSICAL CHEMISTRY; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; TEMPERATURE; WASTE WATER; WATER SAMPLING;

ANIMALS; CRYPTOSPORIDIUM PARVUM; DNA; ENVIRONMENTAL MONITORING; GENOTYPE; GIARDIA LAMBLIA; IMMUNOMAGNETIC SEPARATION; POLYMERASE CHAIN REACTION; WATER;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; GIARDIA; GIARDIA INTESTINALIS; PROTOZOA;

EID: 34447130252     PISSN: 09603123     EISSN: 13691619     Source Type: Journal    
DOI: 10.1080/09603120701372573     Document Type: Article

References (25)

1. Anceno AJ, Shipin OV, Chuluun B, Katayama H. 2007. Relief of inhibitors for the PCR-based monitoring of occurrence and fate of Cryptosporidium in wastewater and surface water. In: Furumai H, Kurisu F, Katayama H, Satoh H, Ohgaki S, Thanh NC, editors. Southeast Asian Water Environment 2. London: IWA Publishing. p 346.
2. Bertrand I, Albertini L, Schwartzbrod J. 2005. Comparison of two target genes for detection and genotyping of Giardia lamblia in human feces by PCR and PCR-restriction fragment length polymorphism. J Clin Microbiol 43:5940-5944.
3. Caccio SM. 2004. New methods for the diagnosis of Cryptosporidium and Giardia: Review. Parassitologia 46:151-155.
4. Carey CM, Lee H, Trevors JT. 2004. Biology, persistence and detection of Cryptosporidium parvum and Cryptosporidium hominis oocyst: Review. Wat Res 38:818-862.
5. Di Giovanni GD, Hashemi FH, Shaw NJ, Abahams FA, LeChevalier MW, Abbaszadegan M. 1999. Detection of infectious Cryptosporidium parvum oocysts in surface and filter backwash water samples by immunomagnetic separation and integrated cell culture-PCR. Appl Environ Microbiol 65:3427-3432.
6. DuPont HL, Chappell CL, Sterling CR, Okhuysen PC, Rose JB, Jakubowski W. 1995. The infectivity of Cryptosporidium parvum in healthy volunteers. New Eng J Med 332:855-859.
7. Fontaine M, Guillot E. 2003. An immunomagnetic separation-real-time PCR method for quantification of Cryptosporidium parvum in water samples. J Microbiol Methods 54:29-36.
8. Greinert JA, Furtado DN, Smith JJ, Monte-Barardi C, Simões CO. 2004. Detection of Cryptosporidium oocysts and Giardia cysts in swimming pool filter backwash water concentrates by flocculation and immunomagnetic separation. Int J Environ Health Res 14:395-404.
9. Guy R, Payment P, Krull U, Horgen P. 2003. Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl Environ Microbiol 69:5178-5185.
10. Hallier-Soulier S, Guillot E. 1999. An immunomagnetic separation polymerase chain reaction assay for rapid and ultra sensitive detection of Cryptosporidium parvum in drinking water. FEMS Microbiol Lett 176:285-289.
11. Jiang J, Alderisio K, Singh A, Xiao L. 2005. Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Appl Environ Microbiol 71:1135-1141.
12. Limsawat S, Ohgaki S. 1997. Fate of liberated viral RNA in wastewater determined by PCR. Appl Environ Microbiol 63:2932-2933.
13. Loge FJ, Thompson DE, Call DR. 2002. PCR detection of specific pathogens in water: A risk-based analysis. Environ Sci Technol 36:754-759.
14. Lowery CJ, Moore JE, Millar BC, Burke DP, McCorry KA, Crothers E, Dooley JSG. 2000. Detection and speciation of Cryptosporidium spp. in environmental samples by immunomagnetic separation, PCR and endonuclease restriction. J Med Microbiol 49:779-785.
15. Morgan UM, Clare C, Constantine CC, Forbes DA, Thompson RCA. 1997. Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis. J Parasitol 83:825-830.
16. Ng CT, Gilchrist CA, Lane A, Roy S, Haque R, Houpt ER. 2005. Multiplex real-time PCR assay using Scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples. J Clin Microbiol 43:1256-1260.
17. Quintero-Betancourt W, Peele E, Rose J. 2002. Cryptosporidium parvum and Cyclospora cayetanensis: A review of laboratory methods for detection of these waterborne parasites. J Microbiol Methods 49:209-224.
18. Rendtorff RC. 1954. The experimental transmission of human intestinal protozoan parasites. II. Giardia lamblia cysts given in capsules. Am J Hyg 59:209-220.
19. Rimhanen-Finne R, Hörman A, Ronkainen P, Hänninen M-L. 2002. An IC-PCR method for detection of Cryptosporidium and Giardia in natural surface waters in Finland. J Microbiol Methods 50:299-303.
20. Rimhanen-Finne R, Ronkainen P, Hänninen M-L. 2001. Simultaneous detection of Cryptosporidium parvum and Giardia in sewage sludge by IC-PCR. J Appl Microbiol 91:1030-1035.
21. Robertson LJ, Paton CA, Campbell AT, Smith PG, Jackson MH, Gilmour RA, Black SE, Stevenson DA, Smith HV. 2000. Giardia cysts and Cryptosporidium oocysts at sewage treatment works in Scotland, UK. Wat Res 34:2310-2322.
22. Rochelle PA, de Leon R, Johnson A, Stewart MC, Wolfe RL. 1999. Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples. Appl Environ Microbiol 65:841-845.
23. Rose J, Dickson L, Farrah S, Carnahan R. 1996. Removal of pathogenic and indicator microorganisms by a full-scale water reclamation facility. Wat Res 30:2785-2797.
24. US Environmental Protection Agency (USEPA). 1999. Method 1623: Cryptosporidium and Giardia in water by Filtration/IMS/IFA. Office of Water. EPA-821-R-99-006.
25. Wiedenmann A, Kruger P, Botzenhart K. 1998. PCR detection of Cryptosporidium parvum in environmental samples - a review of published protocols and current developments. J Ind Microbiol Biotechnol 21:150-166.