Genotyping of Giardia lamblia human isolates from Portugal by PCR-RFLP and sequencing

Journal of Eukaryotic Microbiology

Volumn 53, Issue SUPPL. 1, 2006, Pages

  Sousa, M C ^a   Morais, J B ^a   Machado, J E ^a   Poiares Da Silva, J ^a  

^a UNIVERSITY OF COIMBRA   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSKELETON PROTEIN; GIARDIN PROTEIN, GIARDIA LAMBLIA; PROTOZOAL PROTEIN;

FLAGELLATE; GENETIC ANALYSIS; GENOTYPE; POLYMERASE CHAIN REACTION;

ADOLESCENT; ADULT; AGAR GEL ELECTROPHORESIS; ANIMAL; CHILD; CLASSIFICATION; CONFERENCE PAPER; DNA FINGERPRINTING; DNA SEQUENCE; FECES; FEMALE; GENETICS; GENOTYPE; GIARDIA LAMBLIA; GIARDIASIS; HUMAN; INFANT; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; MIDDLE AGED; PARASITOLOGY; POLYMERASE CHAIN REACTION; PORTUGAL; PRESCHOOL CHILD; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ADOLESCENT; ADULT; ANIMALS; CHILD; CHILD, PRESCHOOL; CYTOSKELETAL PROTEINS; DNA FINGERPRINTING; ELECTROPHORESIS, AGAR GEL; FECES; FEMALE; GENOTYPE; GIARDIA LAMBLIA; GIARDIASIS; HUMANS; INFANT; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; PORTUGAL; PROTOZOAN PROTEINS; SEQUENCE ANALYSIS, DNA;

EURASIA; EUROPE; PORTUGAL; SOUTHERN EUROPE;

GIARDIA INTESTINALIS;

EID: 33751376138     PISSN: 10665234     EISSN: 15507408     Source Type: Journal    
DOI: 10.1111/j.1550-7408.2006.00221.x     Document Type: Conference Paper

References (20)

1. Amar, C. F. L., Dear, P. H., Pedraza-Díaz, S., Looker, N., Linnane, E. & MacLauchlin, J. 2002. Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces. J. Clin. Microbiol., 40:446-452.
2. Aydin, A. F., Besirbellioglu, B. A., Avci, I. Y., Tanyuksel, M., Araz, E. & Pahsa, A. 2004. Classification of Giardia duodenalis parasites in Turkey into groups A and B using restriction fragment length polymorphism. Diagn. Microbiol. Infect. Dis., 50:147-151.
3. Cacciò, S. M., de Giacomo, M. & Pozio, E. 2002. Sequence analysis of the β-giardin gene and development of a PCR-RFLP assay to genotype Giardia duodenalis cysts from human feacal samples. Int. J. Parasitol., 32:3393-3398.
4. Ey, P. L., Mansouri, M., Kulda, J., Nohýnková, E., Monis, P. T., Andrews, R. & Mayrhofer, G. 1997. Genetic analysis of Giardia from hoot farm animals reveals artiodactyl-specific and potentially zoonotic genotypes. J. Euk. Microbiol., 44:626-635.
5. Homan, W. L. & Mank, T. G. 2001. Human giardiasis: genotype linked differences in clinical symptomatology. Int. J. Parasitol., 31:822-826.
6. Keister, D. B. 1983. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans. R. Soc. Trop. Med. Hyg., 77:487-488.
7. Lalle, M., Pozio, E., Capelli, G., Bruschi, F., Crotti, D. & Cacciò, S. M. 2005. Genetic heterogeneity at the β-giardin locus among human and animal isolates of Giardia duodenalis and identification of potencially zoonotic subgenotypes. Int. J. Parasitol., 35:207-213.
8. Lemos, V., Graczyk, T. K., Alves, M. L., Lobo, M. L., Sousa, M. C., Antunes, F. & Matos, O. 2005. Identification and determination of the viability of Giardia lamblia cysts, Cryptosporidium parvum and Cryptosporidium hominis oocysts in human faecal and water supply samples by fluorescent in situ hybridization (FISH) and monoclonal antibodies. Parasitol. Res., 98:48-53.
9. MacLauchlin, J., Pedraza-Díaz, S., Amar-Hoetzeneder, C. & Nichols, G. L. 1999. Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis. J. Clin. Microbiol., 37:3153-3158.
10. MacIntyre, L., Hoang, L., Ong, C. S. L., Lee, P. & Isaac-Renton, J. L. 2000. Evaluation of molecular techniques to biotype Giardia duodenalis collected during an outbreak. J. Parasitol., 86:172-177.
11. MacRoberts, K. M., Meloni, B. P., Morgan, U. M., Marano, R., Binz, N. & Erlandsen, S. L. 1996. Morphological and molecular characterization of Giardia isolated from the straw-necked ibis (Threskiornis spinicollis) in western Australia. J. Parasitol., 82:711-718.
12. Monis, P. T., Andrews, R. H., Mayrhofer, G. & Ey, P. L. 1999. Molecular systematics of the parasitic protozoan Giardia intestinalis. Mol. Biol. Evol., 16(Suppl. 9):1135-1144.
13. Monis, P. T., Andrews, R. H., Mayrhofer, G. & Ey, P. L. 2003. Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect. Genet. Evol., 3:29-38.
14. Paintlia, S. A. & Mahajan, R. C. 1999. Characterization of Giardia lamblia groups A and B from North India by isoenzyme and randon amplified polymorphic DNA analysis. Parasitol. Res., 85:510-512.
15. Read, C., Walters, J., Robertson, I. D. & Thompson, R. C. A. 2002. Correlation between genotype of Giardia duodenalis and diarrhoea. Int. J. Parasitol., 32:229-231.
16. Roberts-Thompson, I. C. & Mitchell, G. F. 1978. Giardiasis in mice. Prolonged infections in certain mouse strains and hypothymic (nude) mice. Gastroenterology, 75:42-46.
17. Sousa, M. C. & Poiares-da-Silva, J. 2004. Characterization of human isolates of Giardia lamblia from Portugal. Abstract. Int. J. Infect. Dis., 8(suppl.1):S218.
18. Sousa, M. C., Donato, A. & Poiares-da-Silva, J. 2002. Genotyping of human isolates of Giardia lamblia. Abstract. Clin. Microbiol. Infect., 8(Suppl. 1):P1273.
19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. 1997. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res., 24:4876-4882.
20. Thompson, R. C. A., Hopkins, R. M. & Homan, W. L. 2000. Nomenclature and genetic groupings of Giardia infecting mammals. Parasitol. Today, 16:210-213.