Development of a sensitive and specific qPCR assay in conjunction with propidium monoazide for enhanced detection of live Salmonella spp. in food

BMC Microbiology

Volumn 13, Issue 1, 2013, Pages

  Li, Baoguang ^a   Chen, Jin Qiang ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

invA gene; Live cells; Propidium monoazide; qPCR; Salmonella

Indexed keywords

PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG; AZIDE; BACTERIAL DNA; BACTERIAL PROTEIN; ENZYME INHIBITOR; INVA PROTEIN, BACTERIA;

AMPLICON; ARTICLE; BACTERIAL GROWTH; BACTERIUM DETECTION; BEEF; CELL DIFFERENTIATION; COLONY FORMING UNIT; CONTROLLED STUDY; DNA EXTRACTION; FOOD ANALYSIS; GENE AMPLIFICATION; LIMIT OF DETECTION; NONHUMAN; POLYMERASE CHAIN REACTION; SALMONELLA; SALMONELLA ENTERITIDIS; SENSITIVITY AND SPECIFICITY; SINGLE NUCLEOTIDE POLYMORPHISM; SPINACH; ANALOGS AND DERIVATIVES; EVALUATION STUDY; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIAL VIABILITY; MICROBIOLOGICAL EXAMINATION; PHYSIOLOGY; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION;

SALMONELLA; SPINACIA OLERACEA;

AZIDES; BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; DNA, BACTERIAL; ENZYME INHIBITORS; FOOD MICROBIOLOGY; MICROBIAL VIABILITY; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION; SALMONELLA; SENSITIVITY AND SPECIFICITY;

EID: 84888359825     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-13-273     Document Type: Article

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