Use of propidium monoazide and increased amplicon length reduce false-positive signals in quantitative PCR for bioburden analysis

Applied Microbiology and Biotechnology

Volumn 97, Issue 5, 2013, Pages 2153-2162

  Schnetzinger, Franz ^a   Pan, Youwen ^b   Nocker, Andreas ^a  

^a CRANFIELD UNIVERSITY   (United Kingdom)

^b BAXTER HEALTHCARE CORPORATION   (United States)

Author keywords

Bioburden analysis; False positive signals; Propidium monoazide; Rapid microbiological method; Real time PCR

Indexed keywords

BIOBURDEN; FALSE-POSITIVE SIGNALS; MICROBIOLOGICAL METHODS; PROPIDIUM; REAL-TIME PCR;

CHEMICAL REACTIONS; DNA; ESCHERICHIA COLI; RNA; SIGNAL DETECTION;

POLYMERASE CHAIN REACTION;

DYE; GENOMIC DNA; PROPIDIUM MONOAZIDE; RNA 16S; UNCLASSIFIED DRUG;

CONCENTRATION (COMPOSITION); DNA; GENOMICS; HEALTH CARE; MEMBRANE; MICROBIAL COMMUNITY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SAMPLING;

AMPLICON; ARTICLE; CELL SUSPENSION; ESCHERICHIA COLI; FALSE POSITIVE RESULT; LIMIT OF DETECTION; MICROBIOLOGICAL PARAMETERS; NUCLEIC ACID AMPLIFICATION; PHOTOACTIVATION; POLYMERASE CHAIN REACTION;

AZIDES; ENZYME INHIBITORS; FALSE POSITIVE REACTIONS; INTERCALATING AGENTS; MICROBIAL VIABILITY; MICROBIOLOGICAL TECHNIQUES; POLYMERASE CHAIN REACTION; PROPIDIUM; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84874110056     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4711-6     Document Type: Article

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