Rapid detection of viable Salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification

Applied and Environmental Microbiology

Volumn 77, Issue 12, 2011, Pages 4008-4016

  Chen, Siyi ^a   Wang, Fei ^a   Beaulieu, John C ^b   Stein, Rebecca E ^b   Ge, Beilei ^a  

^a LOUISIANA STATE UNIVERSITY AGRICULTURAL CENTER   (United States)

^b SOUTHERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUMBER; CELL VIABILITY; CONTROL POTENTIAL; DETECTION LIMITS; DETECTION METHODS; NOVEL STRATEGIES; OPTIMIZED CONDITIONS; PROPIDIUM; PURE CULTURE; QUANTITATIVE REAL TIME PCR; RAPID DETECTION; SAMPLE TREATMENT; SIMPLE METHOD; TIME THRESHOLDS;

AMPLIFICATION; CELL CULTURE; OPTIMIZATION; POLYMERASE CHAIN REACTION;

SALMONELLA;

AZIDE; DRUG DERIVATIVE; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE;

BACTERIUM; BIOASSAY; BIOTECHNOLOGY; CONCENTRATION (COMPOSITION); DETECTION METHOD; DYE; INDUSTRIAL PRODUCTION; ISOTHERM; OPTIMIZATION; POLYMERASE CHAIN REACTION; VIABILITY;

ARTICLE; COMPARATIVE STUDY; EVALUATION; FRUIT; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIAL VIABILITY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION; PHYSIOLOGY; SALMONELLA; SENSITIVITY AND SPECIFICITY; TIME; VEGETABLE;

AZIDES; BACTERIOLOGICAL TECHNIQUES; FRUIT; MICROBIAL VIABILITY; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PROPIDIUM; SALMONELLA; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VEGETABLES;

CUCUMIS MELO; LYCOPERSICON ESCULENTUM; SALMONELLA; SPINACIA OLERACEA;

EID: 79960092323     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00354-11     Document Type: Article

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