Rapid detection of viable bacteria by nested polymerase chain reaction via long DNA amplification after ethidium monoazide treatment

Analytical Biochemistry

Volumn 418, Issue 2, 2011, Pages 286-294

  Soejima, Takashi ^a,b   Schlitt Dittrich, Frank ^b   Yoshida, Shin Ichi ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b MORINAGA MILK INDUSTRY CO LTD   (Japan)

Author keywords

Cells of Enterobacteriaceae; E. sakazakii; EMA; Nested PCR; Pasteurized milk; PMA; Real time PCR

Indexed keywords

BACTERIA; CYTOLOGY; DNA; GENES; PASTEURIZATION; RNA;

E. SAKAZAKII; ENTEROBACTERIACEAE; NESTED PCR; PASTEURIZED MILK; REAL-TIME PCR;

POLYMERASE CHAIN REACTION;

AZIDE; BACTERIAL DNA; DNA 16S; ETHIDIUM; ETHIDIUM AZIDE; RNA 16S; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIAL VIABILITY; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; CONTROLLED STUDY; DIRECT REAL TIME POLYMERASE CHAIN REACTION; ENTEROBACTER SAKAZAKII; ENTEROBACTERIACEAE; FOOD CONTAMINATION; GENE AMPLIFICATION; KLEBSIELLA OXYTOCA; MILK; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

ENTEROBACTERIACEAE;

EID: 80052767867     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.06.033     Document Type: Article

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