Real-time PCR methodology for selective detection of viable Escherichia coli O157: H7CELLS by targeting Z3276 as a genetic marker

Applied and Environmental Microbiology

Volumn 78, Issue 15, 2012, Pages 5297-5304

  Li, Baoguang ^a   Chen, Jin Qiang ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

96-WELL PLATE; DEAD CELLS; E.COLI O157:H7; ESCHERICHIA COLI O157; ESCHERICHIA COLI O157:H7; FOOD MATRIXES; GENETIC MARKERS; GENOMIC DNA; HIGH-THROUGHPUT; NUMBER OF SAMPLES; OPEN READING FRAME; PCR ASSAY; PROPIDIUM; REAL-TIME PCR; REAL-TIME PCR ASSAY; SELECTIVE DETECTION; VIABLE CELLS;

CELLS; CYTOLOGY; DNA; ESCHERICHIA COLI;

POLYMERASE CHAIN REACTION;

AZIDE; DRUG DERIVATIVE; PRIMER DNA; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE;

BIOASSAY; CATTLE; DETECTION METHOD; EPIDEMIOLOGY; FECAL COLIFORM; FOOD SAFETY; GENETIC MARKER; GENOMICS; INHIBITION; POLYMERASE CHAIN REACTION; REAL TIME; VIABILITY;

ARTICLE; ESCHERICHIA COLI O157; FOOD SAFETY; GENETIC MARKER; GENETICS; GROWTH, DEVELOPMENT AND AGING; METHODOLOGY; OPEN READING FRAME; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; STEM CELL;

AZIDES; DNA PRIMERS; ESCHERICHIA COLI O157; FOOD SAFETY; GENETIC MARKERS; OPEN READING FRAMES; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; STEM CELLS;

ESCHERICHIA COLI;

EID: 84866152247     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00794-12     Document Type: Article

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