Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems

Protein Expression and Purification

Volumn 92, Issue 1, 2013, Pages 77-87

  Zelasko, Susan ^a   Palaria, Amrita ^b,d   Das, Aditi ^a,b,c  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c University of Illinois at Urbana Champaign   (United States)

^d UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

Cytochrome P450 protein; Cytochrome P450s; Expression systems; N terminus modifications; Recombinant expression; Silent mutations

Indexed keywords

CYTOCHROME P450; RECOMBINANT PROTEIN;

CHEMICAL STRUCTURE; CHEMISTRY; CODON; CYTOCHROME P450 PROTEIN; CYTOCHROME P450S; ESCHERICHIA COLI; EXPRESSION SYSTEMS; GENE EXPRESSION; GENETICS; HUMAN; METABOLISM; MOLECULAR CLONING; MUTATION; N-TERMINUS MODIFICATIONS; PROTEIN CONFORMATION; RECOMBINANT EXPRESSION; REVIEW; SILENT MUTATIONS;

CYTOCHROME P450 PROTEIN; CYTOCHROME P450S; EXPRESSION SYSTEMS; N-TERMINUS MODIFICATIONS; RECOMBINANT EXPRESSION; SILENT MUTATIONS;

CLONING, MOLECULAR; CODON; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

EID: 84886632399     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.07.017     Document Type: Review

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