Heterologous expression and characterization of wild-type human cytochrome P450 1A2 without conventional N-terminal modification in Escherichia coli

Protein Expression and Purification

Volumn 57, Issue 2, 2008, Pages 188-200

  Kim, Dong Hyun ^a   Kim, Keon Hee ^a   Isin, Emre M ^b   Guengerich, F Peter ^b   Chae, Ho Zoon ^a   Ahn, Taeho ^a   Yun, Chul Ho ^a  

^a Chonnam National University   (South Korea)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Catalytic function; Heterologous expression; Human cytochrome P450 1A2; N terminal membrane anchor domain; N terminal modification; Spin equilibrium

Indexed keywords

CYTOCHROME P450 1A2; DEUTERIUM; ENZYME INHIBITOR; HYDROGEN PEROXIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CODON; DRUG ANTAGONISM; DRUG EFFECT; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETICS; HUMAN; HYDROXYLATION; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; SPECTROPHOTOMETRY; TEMPERATURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CODON; CYTOCHROME P-450 CYP1A2; DEUTERIUM; ENZYME INHIBITORS; ESCHERICHIA COLI; HUMANS; HYDROGEN PEROXIDE; HYDROXYLATION; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; NADP; OXIDATION-REDUCTION; SPECTROPHOTOMETRY; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI;

EID: 37349017983     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.10.010     Document Type: Article

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