Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression

Biotechnology Letters

Volumn 31, Issue 10, 2009, Pages 1589-1593

  Wu, Zhong Liu ^a   Qiao, Jing ^a,b   Zhang, Zhi Gang ^a,b   Guengerich, F Peter ^c   Liu, Yan ^a   Pei, Xiao Qiong ^a  

^a CHENGDU INSTITUTE OF BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Chaperon; Codon; Cytochrome P450; Escherichia coli; Expression

Indexed keywords

CHAPERON; CHAPERONE CO-EXPRESSION; CO-EXPRESSION; CODON; CODON OPTIMIZATION; CYTOCHROME P450; E. COLI; EXPRESSION; EXPRESSION LEVELS; HETEROLOGOUS EXPRESSION; MOLECULAR CHAPERONES; SYNERGETIC EFFECT;

MAMMALS; OPTIMIZATION;

ESCHERICHIA COLI;

CHAPERONE; CYTOCHROME P450; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CODON; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; MAMMAL; METABOLISM;

ANIMALS; CODON; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; GENE EXPRESSION; MAMMALS; MOLECULAR CHAPERONES; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 70350121593     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0059-5     Document Type: Article

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