Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function

Archives of Biochemistry and Biophysics

Volumn 494, Issue 1, 2010, Pages 86-93

  Tang, Zhongmei ^a   Salamanca Pinzón, Sandra Giovanna ^a   Wu, Zhong Liu ^a   Xiao, Yi ^a   Guengerich, F Peter ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cytochrome P450 4F11; Enzyme purification; Heterologous expression; Liquid chromatography mass spectrometry; Metabolomics

Indexed keywords

ARACHIDONIC ACID; CYTOCHROME P450; CYTOCHROME P450 4F11; DOCOSAHEXAENOIC ACID; OLEIC ACID; PALMITIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; DEMETHYLATION; ENZYME PURIFICATION; ESCHERICHIA COLI; FATTY ACID ANALYSIS; HETEROLOGOUS EXPRESSION; HUMAN; HYDROXYLATION; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MASS FRAGMENTOGRAPHY; MASS SPECTROMETRY; METABOLOMICS; NONHUMAN; OXIDATION KINETICS; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BIOCATALYSIS; BIOTRANSFORMATION; CARCINOGENS; CHROMATOGRAPHY, AFFINITY; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; KINETICS; LIVER; MOLECULAR SEQUENCE DATA; SPECTROPHOTOMETRY, ULTRAVIOLET; SUBSTRATE SPECIFICITY; TANDEM MASS SPECTROMETRY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 74149088519     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2009.11.017     Document Type: Article

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