Expression of human cytochrome P450 46A1 in Escherichia coli: Effects of N- and C-terminal modifications

Archives of Biochemistry and Biophysics

Volumn 428, Issue 1, 2004, Pages 99-108

  Mast, Natalia ^a   Andersson, Ulla ^b   Nakayama, Kazuo ^a,c   Bjorkhem, Ingemar ^b   Pikuleva, Irina A ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c Banyu Pharmaceutical Co   (Japan)

Author keywords

Aggregation; C terminal truncation; CYP46A1; Membrane localization; N terminal truncation; P450; Proline rich region

Indexed keywords

BUFFER; CHOLESTEROL; CHOLESTEROL DERIVATIVE; CYTOCHROME P450 46A1; CYTOCHROME P450 ISOENZYME; HYBRID PROTEIN; MONOMER; PROLINE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING KINETICS; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CYTOSOL; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; IONIC STRENGTH; MEMBRANE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; SOLUBILITY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ENZYME ACTIVATION; ESCHERICHIA COLI; HUMANS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; STEROID HYDROXYLASES; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI;

EID: 3042683304     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.abb.2004.05.012     Document Type: Article

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