Biochemical production of biobutanol

Handbook of Biofuels Production: Processes and Technologies

Volumn , Issue , 2011, Pages 221-257

  Kopke M ^a   Durre P ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Butanol; Clostridium acetobutylicum; Clostridium beijerinckii; Fermentation; Solventogenesis

Indexed keywords

BUTENES; FERMENTATION; METABOLIC ENGINEERING; MOLECULAR BIOLOGY;

ANAEROBIC BACTERIUM; BIOTECHNOLOGICAL APPLICATIONS; CLOSTRIDIUM ACETOBUTYLICUM; CLOSTRIDIUM BEIJERINCKII; DOWNSTREAM-PROCESSING; PROCESS TECHNOLOGIES; SOLVENTOGENESIS; TRANSCRIPTIONAL REGULATION;

CLOSTRIDIUM;

EID: 84886072640     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1533/9780857090492.2.221     Document Type: Chapter

References (263)

1. Ali M.K., Rudolph F.B., Bennett G.N. Thermostable xylanase10B from Clostridium acetobutylicum ATCC824. J Ind Microbiol Biotechnol 2004, 31:229-234.
2. Ali M.K., Rudolph F.B., Bennett G.N. Characterization of thermostable Xyn10A enzyme from mesophilic Clostridium acetobutylicum ATCC 824. J Ind Microbiol Biotechnol 2005, 32:12-18.
3. Alsaker K.V., Papoutsakis E.T. Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum. J Bacteriol 2005, 187:7103-7118.
4. Alsaker K.V., Spitzer T.R., Papoutsakis E.T. Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress. J Bacteriol 2004, 186:1959-1971.
5. Andersch W., Bahl H., Gottschalk G. Level of enzymes involved in acetate, butyrate, acetone and butanol formation by Clostridium acetobutylicum. Eur J Appl Microbiol Biotechnol 1983, 18:327-332.
6. Annous B.A., Blaschek H.P. Isolation and characterization of α-amylase derived from starch-grown Clostridium acetobutylicum ATCC 824. J Ind Microbiol 1994, 13:10-16.
7. Atsumi S., Cann A.F., Connor M.R., Shen C.R., Smith K.M., Brynildsen M.P., et al. Metabolic engineering of Escherichia coli for 1-butanol production. Metabolic Eng 2008, 10:305-311.
8. Atsumi S., Hanai T., Liao J.C. Non fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature 2008, 451:86-89.
9. Baer S.H., Blaschek H.P., Smith T.L. Effect of butanol challenge and temperature on lipid composition and membrane fluidity of butanol-tolerant Clostridium acetobutylicum. Appl Environ Microbiol 1987, 53:2854-2861.
10. Baer S.H., Bryant D.L., Blaschek H.P. Electron spin resonance analysis of the effect of butanol on the membrane fluidity of intact cells of Clostridium acetobutylicum. Appl Environ Microbiol 1989, 55:2729-2731.
11. Bahl H., Andersch J., Gottschalk G. Continuous production of acetone and butanol by Clostridium acetobutylicum in a two-stage phosphate limited chemostat. Appl Microbiol Biotechnol 1982, 15:201-205. 15, 201-05 (Erratum 17, 73.
12. Bahl H., Andersch W., Braun K., Gottschalk G. Effect of pH and butyrate concentration on the production of acetone and butanol by Clostridium acetobutylicum grown in continuous culture. Eur J Appl Microbiol Biotechnol 1982, 14:17-20.
13. Bahl H., Dürre P. Clostridia. Biotechnology and Medical Applications 2001, Wiley-VCH Verlag GmbH, Weinheim.
14. Bahl H., Gottwald M., Kuhn A., Rale V., Andersch W., Gottschalk G. Nutritional factors affecting the ratio of solvents produced by Clostridium acetobutylicum. Appl Environ Microbiol 1986, 52:169-172.
15. Ballongue J., Amine J., Maison E., Petitdemange H., Gay R. Induction of acetoacetate decarboxylase in Clostridium acetobutylicum. FEMS Microbiol Lett 1985, 29:273-277.
16. Behrens S., Mitchell W., Bahl H. Molecular analysis of the mannitol operon of Clostridium acetobutylicum encoding a phosphotransferase system and a putative PTS-modulated regulator. Microbiology 2001, 147:75-86.
17. Berezina O.V., Brandt A., Yarotsky S., Schwarz W.H., Zverlov V.V. Isolation of a new butanol-producing Clostridium strain: high level of hemicellulosic activity and structure of solventogenesis genes of a new Clostridium saccharobutylicum isolate. Syst Appl Microbiol 2009, 32:449-459.
18. Birrer G.A., Chesbro W.R., Zsigray R.M. Electro-transformation of Clostridium beijerinckii NRRL B-592 with shuttle plasmid pHR106 and recombinant derivatives. Appl Microbiol Biotechnol 1994, 41:32-38.
19. Bowles L.K., Ellefson W.I. Effects of butanol on Clostridium acetobutylicum. Appl Environ Microbiol 1985, 50:1165-1170.
20. Boynton Z.L., Bennett G.N., Rudolph F.B. Intracellular concentrations of coenzyme A and its derivatives from Clostridium acetobutylicum ATCC 824 and their roles in enzyme regulation. Appl Environ Microbiol 1994, 60:39-44.
21. Boynton Z.L., Bennett G.N., Rudolph F.B. Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 1996, 62:2758-2766.
22. BP, DuPont BP-DuPont Biofuels Fact Sheet Available from:, (accessed 31 Oct 2009). http://www.bp.com/liveassets/bp_internet/globalbp/STAGING/global_assets/downloads/B/BiB_bp_dupont_fact_sheet_jun06.pdf.
23. Brooks J.B., Selin M.J., Alley C.C. Electron capture gas chromatography study of the acid and alcohol products of Clostridium septicum and Clostridium chauvoei. J Clin Microbiol 1976, 3:180-185.
24. Brüggemann H., Gottschalk G. Clostridia. Molecular Biology in the Post-Genomic Era 2009, Caister Academic Press, Norfolk.
25. Brügger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., et al. The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic crenarchaeote growing up to 108°C. Archaea 2007, 2:127-135.
26. Butamax Advanced Biofuels Fact Sheet Available from:, (accessed 31 Oct 2009). http://www.butamax.com/assets/pdf/butamax_advanced_biofuels_llc_fact_sheet.pdf.
27. ButylFuel Butanol Works in your Car Today Available from:, (accessed 31 Oct 2009). http://www.butanolc.startlogic.com/index.html.
28. Cary J.W., Petersen D.J., Papoutsakis E.T., Bennett G.N. Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli. J Bacteriol 1988, 170:4613-4618.
29. Chauvatcharin S., Siripatana C., Seki T., Takagi M., Yoshida T. Metabolism analysis and on-line physiological state diagnosis of acetone-butanol fermentation. Biotechnol Bioeng 1998, 58:561-571.
30. Chen C.-K., Blaschek H.P. Acetate enhances solvent production and prevents degeneration in Clostridium beijerinckii BA101. Appl Microbiol Biotechnol 1999, 52:170-173.
31. Chen C.-K., Blaschek H.P. Effect of acetate on molecular and physiological aspects of Clostridium beijerinckii NCIMB 8052 solvent production and strain degeneration. Appl Microbiol Biotechnol 1999, 65:499-505.
32. Chen J.-S. Properties of acid-and solvent-forming enzymes of clostridia. The Clostridia and Biotechnology 1993, 51-76. Butterworth-Heinemann, Stoneham, MA. D.R. Woods (Ed.).
33. Chen J.-S. Alcohol dehydrogenase: multiplicity and relatedness in the solvent-producing clostridia. FEMS Microbiol Rev 1995, 17:263-273.
34. Chiao J.-S., Sun Z.-H. History of the acetone-butanol-ethanol fermentation industry in China: development of continuous production technology. J Mol Microbiol Biotechnol 2007, 13:12-14.
35. Colby G.D., Chen J.-S. Purification and properties of 3-hydroxybutyryl-coenzyme A dehydrogenase from Clostridium beijerinckii ("Clostridium butylicum") NRRL B593. Appl Environ Microbiol 1992, 58:3297-3302.
36. Cole J.R., Chai B., Farris R.J., Wang Q., Kulam-Syed-Mohideen A.S., McGarrell D.M., et al. The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucl Acids Res 2007, 35:D169-D172.
37. Collins M.D., Lawson P.A., Willems A., Cordoba J.J., Fernandez-Garayzabal J., Garcia P., et al. The phylogeny of the genus Clostridium: Proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 1994, 44:812-826.
38. Cornillot E., Nair R.V., Papoutsakis E.T., Soucaille P. The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain. J Bacteriol 1997, 179:5442-5447.
39. Cortiñas T.I., Micalizzi B., de Guzman A.M. Influence of culture conditions on growth and protective antigenicity of Clostridium chauvoei. J Appl Bacteriol 1994, 77:382-387.
40. Dijk E.-K., Raamsdonk L.M. Butanol Production in a Eukaryotic Cell 2009, International Patent 2009/01360.
41. Donaldson G.K., Huang L.L., Maggio-Hall L.A., Nagarajan V., Nakamura C.E., Suh W. Fermentative Production of Four Carbon Alcohols 2007, International Patent WO2007/041269.
42. Dürre P. New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation. Appl Microbiol Biotechnol 1998, 49:639-648.
43. Dürre P. From Pandora's box to cornucopia: clostridia - a historical perspective. Clostridia. Biotechnology and Medical Applications 2001, 1-17. Wiley-VCH Verlag, Weinheim. H. Bahl, P. Dürre (Eds.).
44. Dürre P. Formation of solvents in clostridia. Handbook on Clostridia 2005, 671-693. CRC Press, Boca Raton, FL. P. Dürre (Ed.).
45. Dürre P. Handbook on Clostridia 2005, CRC Press, Boca Raton, FL.
46. Dürre P. Biobutanol: an attractive biofuel. Biotechnol J 2007, 2:1525-1534.
47. Dürre P., Bahl H. Microbial production of acetone/butanol/isopropanol. Biotechnology 1996, Vol. 6:229-268. VCH Verlagsgesellschaft, Weinheim.
48. Dürre P., Bahl H., Gottschalk G. Membrane processes and product formation in anaerobes. Handbook on Anaerobic Fermentations 1988, 187-206. Marcel Dekker, New York. L.E. Erickson, D.Y.-C. Fung (Eds.).
49. Eckert G., Schügerl K. Continuous acetone-butanol production with direct product removal. Appl Microbiol Biotechnol 1987, 27:221-228.
50. Energy Information Administration International Energy Outlook 2007 2007, US government printing office, Washington, DC.
51. Ennis B.M., Marshall C.T., Maddox I.S., Paterson A.H.J. Continuous product recovery by in-situ gas stripping/condensation during solvent production from whey permeate using Clostridium acetobutylicum. Biotechnol Lett 1986, 8:725-730.
52. Ezeji T.C., Qureshi N., Blaschek H.P. Acetone butanol ethanol (ABE) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. Appl Microbiol Biotechnol 2004, 63:653-658.
53. Ezeji T.C., Qureshi N., Blaschek H.P. Industrially relevant fermentations. Handbook on Clostridia 2005, 797-812. CRC Press, Boca Raton, FL. P. Dürre (Ed.).
54. Ezeji T.C., Qureshi N., Blaschek H.P. Bioproduction of butanol from biomass: from genes to bioreactors. Curr Opin Biotechnol 2007, 18:220-227.
55. Fernbach A., Strange E.H. Improvements in the manufacture of products of fermentation 1911, British Patent 15203.
56. Fernbach A., Strange E.H. Improvement in the manufacture of higher alcohols 1911, British Patent 15204.
57. Fernbach A., Strange E.H. Improvements connected with fermentation processes for the production of acetone, and higher alcohols, from starch, sugars, and other carbohydrate materials 1912, British Patent 15073.
58. Feustel L. Molekularbiologische Untersuchungen zur transkriptionellen Regulation des sol-Operons von Clostridium acetobutylicum 2004, PhD Thesis, University of Ulm, Germany.
59. Fischer R.J., Helms J., Dürre P. Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis. J Bacteriol 1993, 175:6959-6969.
60. Fitz A. Ueber die Gährung des Glycerins. Ber Dtsch Chem Ges 1876, 9:1348-1352.
61. Fitz A. Ueber Schizomyceten-Gährungen II [Glycerin, Mannit, Stärke, Dextrin]. Ber Dtsch Chem Ges 1877, 10:276-283.
62. Fitz A. Ueber Schizomyceten-Gährungen III. Ber Dtsch Chem Ges 1878, 11:42-55.
63. Fitz A. Ueber Spaltpilzgährungen. VII. Mittheilung. Ber Dtsch Chem Ges 1882, 15:867-880.
64. Fontaine L., Meynial-Salles I., Girbal L., Yang X., Croux C., Soucaille P. Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. J Bacteriol 2002, 184:821-830.
65. Förberg C., Häggström L. Control of cell adhesion and activity during continuous production of acetone and butanol with adsorbed cells. Enzyme Microbial Technol 1985, 7:230-234.
66. Freier D., Gottschalk G. L(+)-lactate dehydrogenase of Clostridium acetobutylicum is activated by fructose-1,6-bisphosphate. FEMS Microbiol Lett 1987, 43:229-233.
67. Freier-Schröder D., Wiegel J., Gottschalk G. Butanol formation by Clostridium thermosaccharolyticum at neutral pH. Biotechnol Lett 1989, 11:831-836.
68. Friedl A., Qureshi N., Maddox I.S. Continuous acetone-butanol-ethanol (ABE) fermentation using immobilized cells of Clostridium acetobutylicum in a packed bed reactor and integration with product removal by pervaporation. Biotechnol Bioeng 1991, 38:518-527.
69. Gabriel G.L. Butanol fermentation process. Ind Eng Chem 1928, 20:1063-1067.
70. Garcia A., Iannotti E.L., Fischer J.L. Butanol fermentation liquor production and separation by reverse osmosis. Biotechnol Bioeng 1985, 28:785-791.
71. Geng Q., Park C.H. Pervaporative butanol fermentation by Clostridium acetobutylicum B18. Biotechnol Bioeng 1994, 43:978-986.
72. George H.A., Johnson J.L., Moore W.E.C., Holdeman L.V., Chen J.-S. Acetone, isopropanol, and butanol production by Clostridium beijerinckii (syn. Clostridium butylicum) and Clostridium aurantibutyricum. Appl Environ Microbiol 1983, 45:1160-1163.
73. Gerischer U., Dürre P. Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum. J Bacteriol 1990, 172:6907-6918.
74. Gerischer U., Dürre P. mRNA analysis of the adc gene region of Clostridium acetobutylicum during the shift to solventogenesis. J Bacteriol 1992, 174:426-433.
75. Gevo Gevo Announces First Retrofit of Ethanol Demonstration Plant to Biobutanol for the Oil and Chemical Industries Available from:, (accessed 31 Oct 2009). http://www.gevo.com/news_Retrofit-Demo-Plant-pr_100509.php.
76. Girbal L., Croux C., Vasconcelos V., Soucaille P. Regulation of metabolic shifts in Clostridium acetobutylicum ATCC 824. FEMS Microbiol Rev 1995, 17:287-297.
77. Godin C., Engasser J.M. Two-stage continuous fermentation of Clostridium acetobutylicum: effects of pH and dilution rate. Appl Microbiol Biotechnol 1990, 33:269-273.
78. Gottwald M., Gottschalk G. The internal pH of Clostridium acetobutylicum and its effect on the shift from acid to solvent formation. Arch Microbiol 1985, 143:42-46.
79. Gottwald M., Hippe H., Gottschalk G. Formation of n-butanol from D-glucose by strains of the "Clostridium tetanomorphum" group. Appl Environ Microbiol 1984, 48:573-576.
80. Green E.M., Boynton Z.L., Harris L.M., Rudolph F.B., Papoutsakis E.T., Bennett G.N. Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824. Microbiology 1996, 142:2079-2086.
81. Grethlein A.J., Worden R.M., Jain M.K., Datta R. Evidence for production of n-butanol from carbon monoxide by Butyribacterium methylotrophicum. J Ferment Bioeng 1991, 72:58-60.
82. Grobben N.G., Eggink G., Cuperus F.P., Huizing H.J. Production of acetone, butanol and ethanol (ABE) from potato wastes: fermentation with integrated membrane extraction. Appl Microbiol Biotechnol 1993, 39:494-498.
83. Groot W.J., van den Oever C.E., Kossen N.W.F. Pervaporation for simultaneous product recovery in the butanol/isopropanol batch fermentation. Biotechnol Lett 1984, 6:709-714.
84. Groot W.J., van der Lans R.G.J.M., Luyben K.C.A.M. Batch and continuous butanol fermentation with free cells: integration with product recovery by gas stripping. Appl Microbiol Biotechnol 1989, 32:305-308.
85. Grupe H., Gottschalk G. Physiological events in Clostridium acetobutylicum during the shift from acidogenesis to solventogenesis in continuous culture and presentation of a model for shift induction. Appl Environ Microbiol 1992, 58:3896-3902.
86. Gu Y., Li J., Zhang L., Chen J., Niu L., Yang Y., et al. Improvement of xylose utilization in Clostridium acetobutylicum via expression of the talA gene encoding transaldolase from Escherichia coli. J Biotechnol 2009, 143:284-287.
87. Gutierrez N.A., Maddox I.S., Swoboda H., Schuster K.C., Gapes J.R. Strain comparison and medium preparation for the acetone-butanol-ethanol (ABE) fermentation process using a substrate of potato. Bioresource Technol 1998, 66:263-265.
88. Harris J., Mulder R., Kell D.B., Walter R.P., Morris J.G. Solvent production by Clostridium pasteurianum in media of high sugar content. Biotechnol Lett 1986, 8:889-892.
89. Harris L.M., Blank L., Desai R.P., Welker N.E., Papoutsakis E.T. Fermentation characterization and flux analysis of recombinant strains of Clostridium acetobutylicum with an inactivated solR gene. J Ind Microbiol Biotechnol 2001, 27:322-328.
90. Harris L.M., Desai R.P., Welker N.E., Papoutsakis E.T. Characterization of recombinant strains of the Clostridium acetobutylicum butyrate kinase inactivation mutant: need for new phenomenological models for solventogenesis and butanol inhibition?. Biotechnol Bioeng 2000, 67:1-11.
91. Hartmanis M.G.N. Butyrate kinase from Clostridium acetobutylicum. J Biol Chem 1987, 262:617-621.
92. Hartmanis M.G.N., Gatenbeck S. Intermediary metabolism in Clostridium acetobutylicum: levels of enzymes involved in the formation of acetate and butyrate. Appl Environ Microbiol 1984, 47:1277-1283.
93. Hastings J.H.J. Development of the fermentation industries in Great Britain. Advances in Applied Microbiology 1971, Vol. 14:1-45. Academic Press, New York.
94. Hastings J.H.J. Acetone-butyl alcohol fermentation. Economic Microbiology, Primary Products of Metabolism 1978, Vol. 2:31-45. Academic Press, New York.
95. Heap J.T., Kuehne S.A., Ehsaan M., Cartman S.T., Cooksley C.M., Scott J.C., et al. The ClosTron: Mutagenesis in Clostridium refined and streamlined. J Microbiol Methods 2010, 80:49-55.
96. Heap J.T., Pennington O., Cartman S.T., Carter G.P., Minton N.P. The ClosTron: a universal gene knock-out system for the genus Clostridium. J Microbiol Methods 2007, 70:452-464.
97. Heap J.T., Pennington O., Cartmann S.T., Minton N.P. A modular system for Clostridium shuttle plasmids. J Microbiol Methods 2009, 78:79-85.
98. Herrmann G., Jayamani E., Mai G., Buckel W. Energy conservation via electrontransferring flavoprotein in anaerobic bacteria. J Bacteriol 2008, 190:784-791.
99. Hillmann F., Fischer R.-J., Saint-Prix F., Girbal L., Bahl H. PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum. Mol Microbiol 2008, 68:848-860.
100. Hollergschwandner C. Unterschiede in der Regulation der Sporulation von Clostridium acetobutylicum und Bacillus subtilis 2003, PhD Thesis, University of Ulm, Germany.
101. Holt R.A., Cairns A.J., Morris J.G. Production of butanol by Clostridium puniceum in batch and continuous culture. Appl Microbiol Biotechnol 1988, 27:319-324.
102. Holt R.A., Stephens G.M., Morris J.G. Production of solvents by Clostridium acetobutylicum cultures maintained at neutral pH. Appl Environ Microbiol 1984, 48:1166-1170.
103. Huang K.-X., Huang S., Rudolph F.B., Bennett G.N. Identification and characterization of a second butyrate kinase from Clostridium acetobutylicum ATCC 824. J Mol Microbiol Biotechnol 2000, 2:33-38.
104. Huang L., Gibbins L.N., Forsberg C.W. Transmembrane pH gradient and membrane potential in Clostridium acetobutylicum during growth under acetogenic and solventogenic conditions. Appl Environ Microbiol 1985, 50:1043-1047.
105. Huesemann M., Papoutsakis E.T. Effect of acetoacetate, butyrate, and uncoupling ionophores on growth and product formation of Clostridium acetobutylicum. Biotechnol Lett 1986, 8:37-42.
106. Imperial War Museum Collections How did Horse Chestnuts help the War Effort during the First World War? Available from:, (accessed 31 Oct 2009). http://collections.iwm.org.uk/server/show/ConWebDoc.1267.
107. Ingram L.O. Adaptation of membrane lipids to alcohols. J Bacteriol 1976, 125:670-678.
108. Inui M., Suda M., Kimura S., Yasuda K., Suzuki H., Toda H., et al. Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Appl Microbiol Biotechnol 2008, 77:1305-1316.
109. Ismaiel A.A., Zhu C.-X., Colby G.D., Chen J.-S. Purification and characterization of a primary-secondary alcohol dehydrogenase from two strains of Clostridium beijerinckii. J Bacteriol 1993, 175:5097-5105.
110. Izák P., Schwarz K., Ruth W., Bahl H., Kragl U. Increased productivity of Clostridium acetobutylicum fermentation of acetone, butanol, and ethanol by pervaporation through supported ionic liquid membrane. Appl Microbiol Biotechnol 2008, 78:597-602.
111. JGI Clostridium beijerincki NCIMB 8052 Available from:, (accessed 31 Oct 2009). http://genome.jgi-psf.org/finished_microbes/clobe/clobe.info.html.
112. Jiang Y., Xu C., Dong F., Yang Y., Jiang W., Yang S. Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metab Eng 2009, 11:284-291.
113. Jitesh K., Pangarkar V.G., Niranjan K. Pervaporative stripping of acetone, butanol and ethanol to improve ABE fermentation. Bioseparation 2000, 9:145-154.
114. Jones D.T. Applied acetone-butanol fermentation. Clostridia. Biotechnology and Medical Applications 2001, 125-168. Wiley-VCH Verlag, Weinheim. H. Bahl, P. Dürre (Eds.).
115. Jones D.T. Advances in industrial butanol production 2008, Wageningen, 28 September-1, October 2008.
116. Jones D.T., Keis S. Origins and relationships of industrial solvent-producing clostridial strains. FEMS Microbiol Rev 1995, 17:223-232.
117. Jones D.T., Shirley M., Wu X., Woods D.R. Bacteriophage infections in the industrial acetone butanol (AB) fermentation process. J Mol Microbiol Biotechnol 1986, 2:21-26.
118. Jones D.T., Woods D.R. Acetone-butanol fermentation revisited. Microbiol Rev 1986, 50:484-524.
119. Jones S.W., Paredes C.J., Tracy B., Cheng N., Sillers R., Senger R.S., et al. The transcriptional program underlying the physiology of clostridial sporulation. Genome Biol 2008, 9:R114.
120. Junne S., Klein E., Angersbach A., Goetz P. Electrooptical measurements for monitoring metabolite fluxes in acetone-butanol-ethanol fermentations. Biotechnol Bioeng 2008, 99:862-869.
121. Kanchanatawee S., Maddox I.S. Effect of phosphate and ammonium ion concentrations on solvent production in defined medium by Clostridium acetobutylicum. J Ind Microbiol 1990, 5:272-282.
122. Kashket E.R., Cao Z.-Y. Isolation of a degeneration-resistant mutant of Clostridium acetobutylicum NCIMB 8052. J Bacteriol 1993, 59:4198-4202.
123. Keis S., Shaheen R., Jones D.T. Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov. Int J Syst Evol Microbiol 2001, 51:2095-2103.
124. Killeffer D.H. Butanol and acetone from corn. Ind Eng Chem 1927, 19:46-50.
125. Kim J., Bajpai R., Iannotti E.L. Redox potential in acetone-butanol fermentations. Appl Biochem Biotechnol 1988, 18:175-186.
126. Köpke M. Genetische Veränderung von Clostridium ljungdahlii zur Produktion von 1-Butanol aus Synthesegas 2009, PhD Thesis, University of Ulm, Germany.
127. Krouwel P.G., Groot W.J., Kossen N.W. Continuos IBE fermentation by immobilized growing Clostridium beijerinckii cells in a stirred-tank fermentor. Biotechnol Bioeng 1983, 25:281-299.
128. Largier S.T., Long S., Santangelo J.D., Jones D.T., Woods D.R. Immobilized Clostridium acetobutylicum P262 mutants for solvent production. Appl Environ Microbiol 1985, 50:477-481.
129. Larrayoz M.A., Puigjaner L. Study of butanol extraction through pervaporation in acetobutylic fermentation. Biotechnol Bioeng 1987, 30:692-696.
130. Lee J.Y., Jang Y.S., Lee J., Papoutsakis E.T., Lee S.Y. Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production. Biotechnol J 2009, 4:1432-1440.
131. Lee S.F., Forsberg C.W. Isolation and some properties of a β-D-xylosidase from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 1987, 53:651-654.
132. Lee S.F., Forsberg C.W., Gibbins L.N. Cellulolytic activity of Clostridium acetobutylicum. Appl Environ Microbiol 1985, 50:220-228.
133. Lee S.F., Forsberg C.W., Gibbins L.N. Xylanolytic activity of Clostridium acetobutylicum. Appl Environ Microbiol 1985, 50:1068-1076.
134. Lee S.F., Forsberg C.W., Rattray J.B. Purification of characterization of two endoxylanases from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 1987, 53:644-650.
135. Lee S.M., Cho M.O., Um Y., Sang B.I. Development of real-time PCR primer and probe sets for detecting degenerated and non-degenerated forms of the butanol-producing bacterium Clostridium acetobutylicum ATCC 824. Appl Biochem Biotechnol 2009, 161:75-83.
136. Lemmel S.A., Datta R., Frankiewicz J.R. Fermentation of xylan by Clostridium acetobutylicum. Enzyme Microbial Technol 1986, 8:217-221.
137. Liao J.C., Atsumi S., Brynildsen M.P., Cann A.F., Chou K.J., Shen R.P.C., et al. Butanol Production by Recombinant Microorganisms 2008, International Patent 2008/124523.
138. Lienhardt J., Schripsema J., Qureshi N., Blaschek H.P. Butanol production by Clostridium beijerinckii BA101 in an immobilized cell biofilm reactor: increase in sugar utilization. Appl Biochem Biotechnol 2002, 98-100:591-598.
139. Liou J.S.-C., Blakwill D.L., Drake G.R., Tanner R.S. Clostridium carboxidivorans sp. nov., a solvent-producing clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL1 as Clostridium drakei sp. nov. Int J Syst Evol Microbiol 2005, 55:2085-2091.
140. Lopéz-Contreras A.M., Martens A.A., Szijarto N., Mooibroek H., Claassen P.A., van der Oost J., et al. Production by Clostridium acetobutylicum ATCC 824 of CelG, a cellulosomal glycoside hydrolase belonging to family 9. Appl Environ Microbiol 2003, 69:869-877.
141. Lorenz K. Gezielte Mutagenese des sol-Operons und physiologische Untersuchungen zur enzymatischen Aktivität der Alkohol/Aldehyd-Dehydrogenase E aus Clostridium acetobutylicum 1997, PhD Thesis, University of Ulm, Germany.
142. Maddox I.S., Qureshi N., Gutierrez N.A. Utilization of whey by clostridia and process technology. The Clostridia and Biotechnology 1993, 343-369. Butterworth-Heinemann, Stoneham, MA. D.R. Woods (Ed.).
143. Maddox I.S., Steiner E., Hirsch S., Wessner S., Gutierrez N.A., Gapes J.R., et al. The cause of "acid crash" and "acidogenic fermentations" during the batch acetone-butanol-ethanol (ABE-) fermentation process. J Mol Microbiol Biotechnol 2000, 2:95-100.
144. Marchal R., Blanchet D., Vandecasteele J.P. Industrial optimization of acetone-butanol fermentation: a study of the utilization of Jerusalem artichokes. Appl Microbiol Biotechnol 1985, 23:92-98.
145. Matsumura M., Takehara S., Kataoka H. Continuous butanol/isopropanol fermentation in down-flow column reactor coupled with pervaporation using supported liquid membrane. Biotechnol Bioeng 1992, 39:148-156.
146. McClung L.S., McCoy E. Studies on anaerobic bacteria. VII. The serological reactions of Clostridium acetobutylicum, Cl. felsineum and Cl. roseum. Arch Mikrobiol 1935, 6:239-249.
147. McCoy E., Fred E.B., Peterson W.H., Hastings E.G. A cultural study of the acetone butyl alcohol organism. J Infect Dis 1926, 39:457-484.
148. McCoy E., McClung L.S. Studies on anaerobic bacteria. IV. The nature and systematic position of a new chromogenic Clostridium. Arch Mikrobiol 1935, 6:230-238.
149. McCutchan W.N., Hickey R.J. The butanol-acetone fermentations. Ind Ferment 1954, 1:347-388.
150. Meinecke B., Bertram J., Gottschalk G. Purification and characterization of the pyruvate: ferredoxin-oxidoreductase from Clostridium acetobutylicum. Arch Microbiol 1989, 152:244-250.
151. Mermelstein L.D., Papoutsakis E.T. In vivo methylation in Escherichia coli by the Bacillus subtilis phage ϕ3TI to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 1993, 59:1077-1081.
152. Mermelstein L.D., Papoutsakis E.T., Petersen D.J., Bennett G.N. Metabolic engineering of Clostridium acetobutylicum ATCC 824 for increased solvent production by enhancement of acetone formation enzyme activities using a synthetic acetone operon. Biotechnol Bioeng 1993, 42:1053-1060.
153. Minton N.P., Clarke D.J. Clostridia 1989, Plenum Press, New York.
154. Mitchell W.J. Carbohydrate uptake and utilization by Clostridium beijerinckii NCIMB 8052. Anaerobe 1996, 2:379-384.
155. Mitchell W.J., Shaw J.E., Andrews L. Properties of the glucose phosphotransferase system of Clostridium acetobutylicum NCIB 8052. Appl Environ Microbiol 1991, 57:2534-2539.
156. Mitchell W.J., Tangney M. Carbohydrate uptake by the phosphotransferase system and other mechanisms. Handbook on Clostridia 2005, 153-173. CRC Press, Boca Raton, FL. P. Dürre (Ed.).
157. Monot F., Engasser J.-M., Petitdemange H. Regulation of acetone butanol production in batch and continuous cultures of Clostridium acetobutylicum. Biotechnol Bioeng Symp 1983, 13:207.
158. Moreira A.R., Ulmer D.C., Linden J.C. Butanol toxicity in the butylic fermentation. Biotechnol Bioeng Symp 1981, 11:567-579.
159. Mutschlechner O., Swoboda H., Gapes J.R. Continuous two-stage ABE-fermentation using Clostridium beijerinckii NRRL B592 operating with a growth rate in the first stage vessel close to its maximal value. J Mol Microbiol Biotechnol 2000, 2:101-105.
160. Nair R.V., Bennett G.N., Papoutsakis E.T. Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824. J Bacteriol 1994, 176:871-885.
161. Nair R.V., Green E.M., Watson D.E., Bennett G.N., Papoutsakis E.T. Regulation of the sol locus genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor. J Bacteriol 1994, 181:319-330.
162. Nair R.V., Papoutsakis E.T. Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production. J Bacteriol 1994, 176:5843-5846.
163. Nakas J.P., Schaedle M., Parkinson C.M., Coonley C.E., Tannenbaum S.W. System development for linked-fermentation production of solvents from algal biomass. Appl Environ Microbiol 1983, 46:1017-1023.
164. Nakotte S., Schaffer S., Böhringer M., Dürre P. Electroporation of, plasmid isolation from and plasmid conservation in Clostridium acetobutylicum DSM 792. Appl Microbiol Biotechnol 1998, 50:564-567.
165. Ni Y., Sun Z. Recent progress on industrial fermentative production of acetone-butanol-ethanol by Clostridium acetobutylicum in China. Appl Microbiol Biotechnol 2009, 83:415-423.
166. Nielsen D.R., Leonard E., Yoon S.H., Tseng H.C., Yuan C., Prather K.L. Engineering alternative butanol production platforms in heterologous bacteria. Metabolic Eng 2009, 11:262-273.
167. Nold N. Untersuchungen zur Regulation des sol-Operons in Clostridium acetobutylicum 2008, PhD Thesis, University of Ulm, Germanye.
168. Nölling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., et al. Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J Bacteriol 2001, 183:4823-4838.
169. Ounine K., Petitdemange H., Raval G., Gay R. Regulation and butanol inhibition of D-xylose and D-glucose uptake in Clostridium acetobutylicum. Appl Environ Microbiol 1985, 49:874-878.
170. Paquet V., Croux C., Goma G., Soucaille P. Purification and characterization of the extracellular α-amylase from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 1991, 57:212-218.
171. Park C.-H., Okos M.R., Wankat P.C. Acetone-butanol-ethanol (ABE) fermentation in an immobilized cell trickle bed reactor. Biotechnol Bioeng 1989, 34:18-29.
172. Park C.-H., Okos M.R., Wankat P.C. Characterization of an immobilized cell, trickle bed reactor during long term butanol (ABE) fermentation. Biotechnol Bioeng 1990, 36:207-217.
173. Pasteur L. Quelques résultats nouveaux relatifs aux fermentations acétique et butyrique. Bull Soc Chim Paris 1862, 52-53.
174. Perret S., Casalot L., Fierobe H.P., Tardif C., Sabathe F., Belaich J.P., et al. Production of heterologous and chimeric scaffoldins by Clostridium acetobutylicum ATCC 824. J Bacteriol 2004, 186:253-257.
175. Petersen D.J., Bennett G.N. Purification of acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824 and cloning of the acetoacetate decarboxylase gene in Escherichia coli. Appl Environ Microbiol 1990, 56:3491-3498.
176. Petersen D.J., Welch R.W., Rudolph F.B., Bennett G.N. Molecular cloning of an alcohol (butanol) dehydrogenase gene cluster from Clostridium acetobutylicum ATCC 824. J Bacteriol 1991, 173:1831-1834.
177. Qureshi N., Annous B.A., Ezeji T.C., Karcher P., Maddox I.S. Biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates. Microbial Cell Factories 2005, 4:24.
178. Qureshi N., Blaschek H.P. Production of acetone butanol ethanol (ABE) by a hyper-producing mutant strain of Clostridium beijerinckii BA101 and recovery by pervaporation. Biotechnol Prog 1999, 15:594-602.
179. Qureshi N., Blaschek H.P. Butanol production using Clostridium beijerinckii BA101 hyper-butanol producing mutant strain and recovery by pervaporation. Appl Biochem Biotechnol 2000, 84-86:225-235.
180. Qureshi N., Hughes S., Maddox I.S., Cotta M.A. Energy-efficient recovery of butanol from model solutions and fermentation broth by adsorption. Bioprocess Biosyst Eng 2005, 27:215-222.
181. Qureshi N., Karcher P., Cotta M., Blaschek H.P. High-productivity continuous biofilm reactor for butanol production: effect of acetate, butyrate, and corn steep liquor on bioreactor performance. Appl Biochem Biotechnol 2004, 113-116:713-721.
182. Qureshi N., Li X.-L., Hughes S., Saha B.C., Cotta M.A. Butanol production from corn fiber xylan using Clostridium acetobutylicum. Biotechnol Prog 2006, 22:673-680.
183. Qureshi N., Maddox I.S. A mathematical model of a fluidized bed reactor for the continuous production of solvents by immobilized Clostridium acetobutylicum. J Chem Technol Biotechnol 1989, 48:369-378.
184. Qureshi N., Maddox I.S. Reduction in butanol inhibition by perstraction: utilization of concentrated lactose/whey permeate by Clostridium acetobutylicum to enhance butanol fermentation economics. Food and Bioproducts Processing 2005, 83:43-52.
185. Qureshi N., Paterson A.H.J., Maddox I.S. Model for continuous production of solvents from whey permeate in a packed bed reactor using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar. Appl Microbiol Biotechnol 1988, 29:323-328.
186. Qureshi N., Schripsema J., Lienhardt J., Blaschek H.P. Continuous solvent production by Clostridium beijerinckii BA101 immobilized by absorption onto brick. World J Microbiol Biotechnol 2000, 16:230-235.
187. Rao G., Mutharasan R. Alcohol production by Clostridium acetobutylicum induced by methyl viologen. Biotechnol Lett 1986, 8:893-896.
188. Rao G., Mutharasan R. Altered electron flow in continuous cultures of Clostridium acetobutylicum induced by viologen dyes. Appl Environ Microbiol 1987, 53:1232-1235.
189. Ravagnani A., Jennert K.C., Steiner E., Grünberg R., Jefferies J.R., Wilkinson S.R., et al. Spo0A directly controls the switch from acid to solvent production in solvent-forming clostridia. Mol Microbiol 2000, 37:1172-1185.
190. Renewable Fuels Association The Industry - Statistics Available from:, (accessed 31 Oct 2009). http://www.ethanolrfa.org/industry/statistics/.
191. Rood J.I., McClane B.A., Songer J.G., Titball R.W. The Clostridia. Molecular Biology and Pathogenesis 1997, Academic Press, San Diego/London.
192. Ross D. The acetone-butanol fermentation. Prog Ind Microbiol 1961, 3:73-85.
193. Rühl J., Schmid A., Blank L.M. Selected Pseudomonas putida strains able to grow in the presence of high butanol concentrations. Appl Environ Microbiol 2009, 75:4653-4656.
194. Sabathé F., Bélaich A., Soucaille P. Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. FEMS Microbiol Lett 2002, 217:15-22.
195. Sabathé F., Croux C., Cornillot E., Soucaille P. amyP, a reporter gene to study strain degeneration in Clostridium acetobutylicum ATCC 824. FEMS Microbiol Lett 2002, 210:93-98.
196. Sabathé F., Soucaille P. Characterization of the CipA scaffolding protein and in vivo production of a minicellulosome in Clostridium acetobutylicum. J Bacteriol 2003, 185:1092-1096.
197. Schaffer S., Isci N., Zickner B., Dürre P. Changes in protein synthesis and identification of proteins specifically induced during solventogenesis in Clostridium acetobutylicum. Electrophoresis 2002, 23:110-121.
198. Schuster K.C., Goodacre R., Gapes J.R., Young M. Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells. J Ind Microbiol Biotechnol 2001, 27:314-322.
199. Schwarz K., Fiedler T., Fischer R.J., Bahl H. A standard operating procedure (SOP) for the preparation of intra-and extracellular proteins of Clostridium acetobutylicum for proteome analysis. J Microbiol Methods 2007, 68:396-402.
200. Scotcher M.C., Huang K.-X., Harrison M.L., Rudolph F.B., Bennett G.N. Sequences affecting the regulation of solvent production in Clostridium acetobutylicum. J Ind Microbiol Biotechnol 2003, 30:414-420.
201. Seedorf H., Fricke W.F., Veith B., Brüggemann H., Liesegang H., Strittmatter A., et al. The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features. Proc Natl Acad Sci U S A 2008, 105:2128-2133.
202. Senger R.S., Papoutsakis E.T. Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis. Biotechnol Bioeng 2008, 101:1036-1052.
203. Senger R.S., Papoutsakis E.T. Genome-scale model for Clostridium acetobutylicum: Part II. Development of specific proton flux states and numerically determined sub-systems. Biotechnol Bioeng 2008, 101:1053-1071.
204. Shao L., Hu S., Yang Y., Gu Y., Chen J., Yang Y., et al. Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Cell Res 2007, 17:963-965.
205. Shi Z., Blaschek H.P. Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 and the hyper-butanol-producing mutant BA101 during the shift from acidogenesis to solventogenesis. Appl Environ Microbiol 2008, 74:7709-7714.
206. Shinto H., Tashiro Y., Yamashita M., Kobayashi G., Sekiguchi T., Hanai T., et al. Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production. J Biotechnol 2007, 131:45-56.
207. Sillers R., Al-Hinai M.A., Papoutsakis E.T. Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations. Biotechnol Bioeng 2009, 102:38-49.
208. Sillers R., Chow A., Tracy B., Papoutsakis E.T. Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance. Metabolic Eng 2008, 10:321-332.
209. Soucaille P. Process for the Biological Production of n-Butanol with High Yield 2008, International Patent WO2008/052973.
210. Soucaille P., Figge R., Croux C. Process for Chromosomal Integration and DNA Sequence Replacement in Clostridia 2008, International Patent WO2008/040387.
211. Steen E.J., Chan R., Prasad N., Myers S., Petzold C.J., Redding A., et al. Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol. Microbial Cell Factories 2008, 7:36.
212. Stim-Herndon K.P., Petersen D.J., Bennett G.N. Characterization of an acetyl-CoA acetyltransferase (thiolase) gene from Clostridium acetobutylicum ATCC 824. Gene 1995, 154:81-85.
213. Sullivan L., Bennett G.N. Proteome analysis and comparison of Clostridium acetobutylicum ATCC 824 and Spo0A strain variants. J Ind Microbiol Biotechnol 2006, 33:298-308.
214. Tangney M., Brehm J.K., Minton N.P., Mitchell W.J. A gene system for glucitol transport and metabolism in Clostridium beijerinckii NCIMB 8052. Appl Environ Microbiol 1998, 64:1612-1619.
215. Tangney M., Mitchell W.J. Analysis of a catabolic operon for sucrose transport and metabolism in Clostridium acetobutylicum ATCC 824. J Mol Microbiol Biotechnol 2000, 2:71-80.
216. Tangney M., Mitchell W.J. Characterisation of a glucose phosphotransferase system in Clostridium acetobutylicum ATCC 824. Appl Microbiol Biotechnol 2007, 74:398-405.
217. Tangney M., Rousse C., Yazdanian M., Mitchell W.J. Note: sucrose transport and metabolism in Clostridium beijerinckii NCIMB 8052. J Appl Microbiol 1998, 84:914-919.
218. Tangney M., Winters G.T., Mitchell W.J. Characterization of a maltose transport system in Clostridium acetobutylicum ATCC 824. J Ind Microbiol Biotechnol 2001, 27:298-306.
219. Tashiro Y., Takeda K., Kobayashi G., Sonomoto K., Ishizaki A., Yoshino S. High butanol production by Clostridium saccharoperbutylacetonicum N1-4 in fed-batch culture with pH-stat continuous butyric acid and glucose feeding method. J Biosci Bioeng 2004, 98:263-268.
220. Terracciano J.S., Kashket E.R. Intracellular conditions required for initiation of solvent production by Clostridium acetobutylicum. Appl Environ Microbiol 1986, 52:86-91.
221. Thompson D.K., Chen J.-S. Purification and properties of an acetoacetyl coenzyme A-reacting phosphotransbutyrylase from Clostridium beijerinckii ("Clostridium butylicum") NRRL B593. Appl Environ Microbiol 1990, 56:607-613.
222. Thormann K. Untersuchungen zur Regulation des sol-Operons von Clostridium acetobutylicum 2001, PhD Thesis, University of Ulm, Germany.
223. Thormann K., Dürre P. Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?. J Ind Microbiol Biotechnol 2002, 27:307-313.
224. Thormann K., Feustel L., Lorenz K., Nakotte S., Dürre P. Control of butanol formation in Clostridium acetobutylicum by transcriptional activation. J Bacteriol 2002, 184:1966-1973.
225. Tomas C.A., Alsaker K.V., Bonarius H.P., Hendriksen W.T., Yang H., Beamish J.A., et al. DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5. J Bacteriol 2003, 185:4539-4547.
226. Tomas C.A., Beamish J., Papoutsakis E.T. Transcriptional analysis of butanol stress and tolerance in Clostridium acetobutylicum. J Bacteriol 2004, 186:2006-2018.
227. Tomas C.A., Tummala S.B., Papoutsakis E.T. Metabolic engineering of solventogenic clostridia. Handbook on Clostridia 2005, 813-830. CRC Press, Boca Raton, FL. P. Dürre (Ed.).
228. Tomas C.A., Welker N.E., Papoutsakis E.T. Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and changes in the cell's transcriptional program. Appl Environ Microbiol 2003, 69:4951-4965.
229. Toth J., Ismaiel A.A., Chen J.-S. The ald gene, encoding a coenzyme A-acylating aldehyde dehydrogenase, distinguishes Clostridium beijerinckii and two other solvent-producing clostridia from Clostridium acetobutylicum. Appl Environ Microbiol 1999, 65:4973-4980.
230. Tummala S.B., Junne S.G., Papoutsakis E.T. Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations. J Bacteriol 2003, 185:3644-3653.
231. Tummala S.B., Junne S.G., Paredes C.J., Papoutsakis E.T. Transcriptional analysis of product-concentration driven changes in cellular programs of recombinant Clostridium acetobutylicum strains. Biotechnol Bioeng 2003, 84:842-854.
232. Tummala S.B., Tomas C., Harris L.M., Papoutsakis E.T., Welker N.E., Rudolph F.B., et al. Genetic tools for solventogenic clostridia. Clostridia. Biotechnology and Medical Applications 2005, 105-123. Wiley-VCH Verlag, Weinheim. H. Bahl, P. Dürre (Eds.).
233. Tyurin M., Padda R., Huang K.-X., Wardwell S., Caprette D., Bennett G.N. Electrotransformation of Clostridium acetobutylicum ATCC 824 using high-voltage radio frequency modulated square pulses. J Appl Microbiol 2000, 88:220-227.
234. Ullmann S., Dürre P. Changes in DNA topology are involved in triggering the onset of solventogenesis in Clostridium acetobutylicum. Recent Res Dev Microbiol 1998, 2:281-294.
235. Ullmann S., Kuhn A., Dürre P. DNA topology and gene expression in Clostridium acetobutylicum: implications for the regulation of solventogenesis. Biotechnol Lett 1996, 18:1413-1418.
236. Voget C.E., Mignone C.F., Ertola R.J. Butanol production from apple pomace. Biotechnol Lett 1985, 7:43-46.
237. Vollherbst-Schneck K., Sands J.A., Montenecourt B.S. Effect of butanol on lipid composition and fluidity of Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 1984, 47:193-194.
238. Votruba J., Volesky B., Yerushalmi L. Mathematical model of a batch acetone-butanol fermentation. Biotechnol Bioeng 1986, 28:247-255.
239. Wagner E.G., Simons R.W. Antisense RNA control in bacteria, phages, and plasmids. Annu Rev Microbiol 1994, 48:713-742.
240. Walter K.A., Bennett G.N., Papoutsakis E.T. Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes. J Bacteriol 1992, 174:7149-7158.
241. Walter K.A., Mermelstein L.D., Papoutsakis E.T. Studies of recombinant Clostridium acetobutylicum with increased dosages of butyrate formation genes. Ann N Y Acad Sci 1994, 721:69-72.
242. Walter K.A., Nair R.V., Cary J.W., Bennett G.N., Papoutsakis E.T. Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824. Gene 1993, 134:107-111.
243. Wang J.Y., Syvanen M. DNA twist as a transcriptional sensor for environmental changes. Mol Microbiol 1992, 6:1861-1896.
244. Waterson R.M., Castellino F.C., Hass G.M., Hill R.M. Purification and characterization of crotonase from Clostridium acetobutylicum. J Biol Chem 1972, 247:5266-5271.
245. Weizmann C. Improvements in the Bacterial Fermentation of Carbohydrates and in Bacterial Cultures for the Same 1915, British Patent No. 4845.
246. Welsh F.W., Williams R.E., Veliky I.A. Solid carriers for a Clostridium acetobutylicum that produced acetone and butanol. Enzyme Microbial Technol 1987, 9:500-502.
247. Weyer E.R., Rettger L.F. A comparative study of six different strains of the organism commonly concerned in large-scale production of butyl alcohol and acetone by the biological process. J Bacteriol 1927, 14:399-424.
248. Wiesenborn D.P., Rudolph F.B., Papoutsakis E.T. Thiolase from Clostridium acetobutylicum ATCC 824 and its role in the synthesis of acids and solvents. Appl Environ Microbiol 1988, 54:2717-2722.
249. Wiesenborn D.P., Rudolph F.B., Papoutsakis E.T. Coenzyme A transferase from Clostridium acetobutylicum ATCC 824 and its role in the uptake of acids. Appl Environ Microbiol 1989, 55:323-329.
250. Wiesenborn D.P., Rudolph F.B., Papoutsakis E.T. Phosphotransbutyrylase from Clostridium acetobutylicum ATCC 824 and its role in acidogenesis. Appl Environ Microbiol 1989, 55:317-322.
251. Winzer K., Lorenz K., Dürre P. Acetate kinase from Clostridium acetobutylicum: a highly specific enzyme that is actively transcribed during acidogenesis and solventogenesis. Microbiology 1997, 143:3279-3286.
252. Winzer K., Lorenz K., Zickner B., Dürre P. Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. J Mol Microbiol Biotechnol 2000, 2:531-541.
253. Wong J., Bennett G.N. The effect of novobiocin on solvent production by Clostridium acetobutylicum. J Ind Microbiol 1996, 16:354-359.
254. Woods D.R. The clostridia and biotechnology 1993, Butterworth-Heinemann, Stoneham, MA.
255. Yan R.-T., Chen J.-S. Coenzyme A-acylating aldehyde dehydrogenase from Clostridium beijerinckii NRRL B592. Appl Environ Microbiol 1990, 56:2591-2599.
256. Yang X., Tsao G.T. Enhanced acetone-butanol fermentation using repeated fed-batch operation coupled with cell recycle by membrane and simultaneous removal of inhibitory products by adsorption. Biotechnol Bioeng 1995, 47:444-450.
257. Yu Y., Tangney M., Aass H.C., Mitchell W.J. Analysis of the mechanism and regulation of lactose transport and metabolism in Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 2007, 73:1842-1850.
258. Zhang Y., Ma Y., Yang F., Zhang C. Continuous acetone-butanol-ethanol production by corn stalk immobilized cells. J Ind Microbiol Biotechnol 2009, 36:1117-1121.
259. Zhao Y., Tomas C.A., Rudolph F.B., Papoutsakis E.T., Bennett G.N. Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and their implications for solvent formation. Appl Environ Microbiol 2005, 71:530-537.
260. Zickner B., Böhringer M., Nakotte S., Schaffer S., Thormann K., Dürre P. Multiple levels of regulation of solventogenesis in Clostridium acetobutylicum. Regulatory Networks in Prokaryotes 1993, 127-132. Horizon Scientific Press, Wymondham. P. Dürre, B. Friedrich (Eds.).
261. Zillig W., Holz I., Janekovic D., Klenk H.-P., Imsel E., Trent J., et al. Hyperthermus butylicus, a hyperthermophilic sulfur-reducing archaebacterium that ferments peptides. J Bacteriol 1990, 172:3959-3965.
262. Zoutberg G.R., Willemsberg R., Smit G., Teixeira de Mattos M.J., Neijssel O.M. Solvent production by an aggregate-forming variant of Clostridium butyricum. Appl Microbiol Biotechnol 1989, 32:22-26.
263. Zverlov V.V., Berezina O., Velikodvorskaya G.A., Schwarz W.H. Bacterial acetone and butanol production by industrial fermentation in the Soviet Union: use of hydrolyzed agricultural waste for biorefinery. Appl Microbiol 2006, 71:587-597.