Development of real-time PCR primer and probe sets for detecting degenerated and non-degenerated forms of the butanol-producing bacterium clostridium acetobutylicum ATCC 824

Applied Biochemistry and Biotechnology

Volumn 161, Issue 1-8, 2010, Pages 75-83

  Lee, Sun Mi ^a   Cho, Min Ok ^a   Um, Youngsoon ^a   Sang, Byoung In ^a  

^a Korea Institute of Science and Technology   (South Korea)

Author keywords

Biobutanol; Degeneration; Monitoring; pSOL1 plasmid; Real time qPCR

Indexed keywords

16S RDNA SEQUENCE; BIOBUTANOL; BUTANOL FERMENTATION; CLOSTRIDIUM ACETOBUTYLICUM; DEGENERATION; ESSENTIAL GENE; LIMITING FACTORS; PSOL1 PLASMID; REAL-TIME PCR; REAL-TIME QPCR; SOLVENT PRODUCTION; SPECIFIC DETECTION; SPECIFIC PRIMERS;

CLOSTRIDIUM; DNA; GENES; MONITORING; POLYMERASE CHAIN REACTION; PROBES;

ALCOHOLS;

BUTANOL; PRIMER DNA; RIBOSOME DNA;

ARTICLE; BACTERIUM DETECTION; CLOSTRIDIUM ACETOBUTYLICUM; GENE SEQUENCE; MEDICAL RESEARCH; MOLECULAR PROBE; MONITORING; NONHUMAN; PLASMID; REAL TIME POLYMERASE CHAIN REACTION; DNA PROBE; EVALUATION; FERMENTATION; GENETICS; METABOLISM; METHODOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM ACETOBUTYLICUM ATCC 824;

BUTANOLS; CLOSTRIDIUM ACETOBUTYLICUM; DNA PRIMERS; DNA PROBES; FERMENTATION; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 77952995796     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-009-8788-4     Document Type: Article

References (13)

1. Lee, S. M., Cho, M. O., Park, C. H., Chung, Y. C., Kim, J. H., Sang, B. I., et al. (2008). Energy & Fuels, 22, 3459-3464.
2. Schuster, K. C., Goodacre, R., Gapes, J. R., & Young, M. (2001). Journal of Industrial Microbiology & Biotechnology, 27, 314-321.
3. Jones, D. T., & Woods, D. R. (1986). Microbiology and Molecular Biology Reviews, 50, 484-524.
4. Sabathe, F., Croux, C., Cornillot, E., & Soucaille, P. (2002). FEMS Microbiology Letters, 210, 93-98.
5. Chen, C. K., & Blaschek, H. P. (1999). Applied and Environmental Microbiology, 65, 499-505.
6. Chen, C. K., & Blaschek, H. P. (1999). Applied Microbiology and Biotechnology, 52, 170-173.
7. Assobhei, O., Kanouni, A. E., Ismaili, M., Loutfi, M., & Petitdemange, H. (1998). Journal of Fermentation and Bioengineering, 85, 209-212.
8. Cornillot, E., Nair, R. V., Papoutsakis, E. T., & Soucaille, P. (1997). The Journal of Bacteriology, 179, 5442-5447.
9. Giulietti, A., Overbergh, L., Valckx, D., Decallonne, B., Bouillon, R., & Mathieu, C. (2001). Methods, 25, 386-401.
10. Harms, G., Layton, A. C., Dionisi, H. M., Gregory, I. R., Garrett, V. M., Hawkins, S. A., et al. (2003). Environmental Science & Technology, 37, 343-351.
11. Yu, Y., Lee, C., Kim, J., & Hwang, S. (2005). Biotechnology and Bioengineering, 89, 670-679.
12. Labrenz, M., Brettar, I., Christen, R., Flavier, S., Botel, J., & Hofle, M. G. (2004). Applied and Environmental Microbiology, 70, 4971-4979.
13. Lim, J., Do, H., Shin, S. G., & Hwang, S. (2008). Biotechnology and Bioengineering, 99, 1374-1383.