Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in clostridium acetobutylicum fermentations

Biotechnology and Bioengineering

Volumn 102, Issue 1, 2009, Pages 38-49

  Sillers, Ryan ^a   Al Hinai, Mohab Ali ^b   Papoutsakis, Eleftherios T ^a,c  

^a Northwestern University   (United States)

^b SULTAN QABOOS UNIVERSITY   (Oman)

^c University of Delaware   (United States)

Author keywords

Acetyl CoA; Butyryl CoA; Clostridium acetobutylicum; Flux analysis; Metaboli engineering; Thiolase

Indexed keywords

ACETYL-COA; BUTYRYL-COA; CLOSTRIDIUM ACETOBUTYLICUM; FLUX ANALYSIS; METABOLI ENGINEERING; THIOLASE;

ACETONE; ALCOHOLS; ALDEHYDES; BIOCHEMICAL ENGINEERING; COMPOSITE MICROMECHANICS; ENZYMES; ETHANOL; GENE ENCODING; METABOLISM; NUCLEIC ACIDS; RNA; SOLVENTS;

ORGANIC SOLVENTS;

ACETONE; ACETYL COENZYME A ACETYLTRANSFERASE; ALCOHOL; ALCOHOL DEHYDROGENASE; ALDEHYDE DEHYDROGENASE; BUTANOL; BUTYRIC ACID; COENZYME A TRANSFERASE; ACETOACETYL COENZYME A; ACYL COENZYME A; BACTERIAL PROTEIN; UNCLASSIFIED DRUG;

ALCOHOL PRODUCTION; ARTICLE; CLOSTRIDIUM ACETOBUTYLICUM; CONTROLLED STUDY; DNA MODIFICATION; FERMENTATION; GENE EXPRESSION; METABOLIC ENGINEERING; NONHUMAN; BIOSYNTHESIS; GENETICS; METABOLISM;

CLOSTRIDIUM ACETOBUTYLICUM;

ACETONE; ACETYL-COA C-ACETYLTRANSFERASE; ACYL COENZYME A; ALDEHYDE DEHYDROGENASE; BACTERIAL PROTEINS; BUTANOLS; CLOSTRIDIUM ACETOBUTYLICUM; ETHANOL; FERMENTATION; GENE EXPRESSION; METABOLIC NETWORKS AND PATHWAYS;

EID: 58149268329     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22058     Document Type: Article

References (27)

1. Alsaker KV, Papoutsakis ET. 2005. Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum. J Bacteriol 187(20):7103-7118.
2. Alsaker KV, Spitzer TR, Papoutsakis ET. 2004. Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress. J Bacteriol 186(7):1959-1971.
3. Alsaker K, Paredes CJ, Papoutsakis ET. 2005. Design, optimization, and validation of genomic DNA microarrays for examining the Clostridium acetobutylicum transcriptome. Biotechnol Bioprocess Eng 10(5):432- 443.
4. Boynton ZL, Bennett GN, Rudolph FB. 1996. Cloning, sequencing, and expression of clustered genes encoding β-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. J Bacteriol 178(11): 3015-3024.
5. Clark SW, Bennett GN, Rudolph FB. 1989. Isolation and characterization of mutants of Clostridium acetobutylicum ATCC 824 deficient in acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase (EC 2.8.3.9) and in other solvent pathway enzymes. Appl Environ Microbiol 55(4):970-976.
6. Cornillot E, Nair RV, Papoutsakis ET, Soucaille P. 1997. The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain. J Bacteriol 179(17):5442-5447.
7. Desai RP, Papoutsakis ET. 1999. Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum. Appl Environ Microbiol 65(3):936-945.
8. Desai RP, Harris LM, Welker NE, Papoutsakis ET. 1999a. Metabolic flux analysis elucidates the importance of the acid-formation pathways in regulating solvent production by Clostridium acetobutylicum. Metab Eng 1(3):206-213.
9. Desai RP, Nielsen LN, Papoutsakis ET. 1999b. Metabolic flux analysis of C. acetobutylicum fermentations using nonlinear constraints. J Biotechnol 71:191-205.
10. Gerischer U, D̈rre P. 1990. Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum. J Bacteriol 172(12):6907-6918.
11. Harris LM, Desai RP, Welker NE, Papoutsakis ET. 2000. Characterization of recombinant strains of the Clostridium acetobutylicum butyrate kinase inactivation mutant: Need for new phenomenological models for solventogenesis and butanol inhibition? Biotechnol Bioeng 67(1): 1-11.
12. Harris LM, Welker NE, Papoutsakis ET. 2002. Northern, morphological, and fermentation analysis of spoOA inactivation and overexpression in Clostridium acetobutylicum ATCC 824. J Bacteriol 184(13):3586-3597.
13. Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. 2007. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods 70(3):452-464.
14. Husemann MHW, Papoutsakis ET. 1988. Solventogenesis in Clostridium-Acetobutylicum fermentations related to carboxylic-acid and proton concentrations. Biotechnol Bioeng 32(7):843-852.
15. Mermelstein LD, Papoutsakis ET. 1993. In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium aceto- butylicum ATCC 824. Appl Environ Microbiol 59(4):107710-107781.
16. Mermelstein LD, Welker NE, Bennett GN, Papoutsakis ET. 1992. Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824. Biotechnology (NY) 10(2):190-195.
17. Nair RV, Bennett GN, Papoutsakis ET. 1994. Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium-Acetobu-tylicum Atcc-824. J Bacteriol 176(3):871-885.
18. Nölling J, Breton G, Omelchenko MV, Makarova KS, Zeng Q, Gibson R, Lee HM, Dubois J, Qiu D, Hitti J, Wolf YI, Tatusov RL, Sabathe F, Doucette-Stamm L, Soucaille P, Daly MJ, Bennett GN, Koonin EV, Smith DR. 2001. Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J Bacteriol 183(6):4823-4838.
19. Qureshi N, Blaschek HP. 2001. Recent advances in ABE fermentation: Hyper-butanol producing Clostridium beijerinckii BA101. J Ind Microbiol Biotechnol 27(5):287-291.
20. Shao L, Hu S, Yang Y, Gu Y, Chen J, Yang Y, Jiang W, Yang S. 2007. Targeted gene disruption by use ofa group II intron (targetron) vector in Clostridium acetobutylicum. Cell Res 17(11):963-965.
21. Tomas CA, Alsaker KV, Bonarius HPJ, Hendriksen WT, Yang H, Beamish JA, Parades CJ, Papoutsakis ET. 2003a. DNA-array based transcriptional analysis of asporogenous, non-solventogenic Clostridium acetobutylicum strains SKO1 and M5. J Bacteriol 185(15):4539-4547.
22. Tomas CA, Beamish JA, Papoutsakis ET. 2003b. Transcriptional analysis of butanol stress and tolerance in Clostridium acetobutylicum. J Bacteriol 186(7):2006-2018.
23. Tomas CA, Welker NE, Papoutsakis ET. 2003c. Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and large changes in the cell's transcriptional program. Appl Environ Microbiol 69(8):4951-4965.
24. Tummala SB, Welker NE, Papoutsakis ET. 1999. Development and characterization of a gene expression reporter system for Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 65(9):3793-3799.
25. Tummala SB, Junne SG, Papoutsakis ET. 2003a. Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations. J Bacteriol 185(12):3644-3653.
26. Tummala SB, Welker NE, Papoutsakis ET. 2003b. Design ofantisense RNA constructs for downregulation of the acetone formation pathway of Clostridium acetobutylicum. J Bacteriol 185(6):1923-1934.
27. Zhao YS, Tomas CA, Rudolph FB, Papoutsakis ET, Bennett GN. 2005. Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and their implications for solvent formation. Appl Environ Microbiol 71(1):530-537.