Directed evolution of a fluorogen-activating single chain antibody for function and enhanced brightness in the cytoplasm

Molecular Biotechnology

Volumn 54, Issue 3, 2013, Pages 829-841

  Yates, Bradley P ^a   Peck, Michelle A ^a   Berget, Peter B ^a,b  

^a CARNEGIE MELLON UNIVERSITY   (United States)

^b UNIVERSITY OF THE SCIENCES   (United States)

Author keywords

Directed evolution; Fluorogen activating protein; Fluorogenic dyes; Fluoromodule; Intrabody; scFv

Indexed keywords

DIRECTED EVOLUTION; FLUOROGENICS; FLUOROMODULE; INTRABODY; SCFV;

AMINO ACIDS; CELLS; CHAINS; CHEMICAL ACTIVATION; COVALENT BONDS; CYTOLOGY; LUMINANCE; OPTIMIZATION;

ANTIBODIES;

CYSTEINE; DISULFIDE; FLUOROGEN ACTIVATIG PROTEIN; PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY STRUCTURE; ANTIGEN SPECIFICITY; ARTICLE; CYTOPLASM; DISULFIDE BOND; FLOW CYTOMETRY; FLUOROMETRY; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BIOTECHNOLOGY; CYTOLOGICAL TECHNIQUES; CYTOPLASM; DIRECTED MOLECULAR EVOLUTION; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; ROSANILINE DYES; SEQUENCE ALIGNMENT; SINGLE-CHAIN ANTIBODIES;

EID: 84882894834     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-012-9631-7     Document Type: Article

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