In vitro evolution of an antibody fragment population to find high-affinity hapten binders

Protein Engineering, Design and Selection

Volumn 21, Issue 8, 2008, Pages 485-493

  Persson, Helena ^a   Wallmark, Henrik ^a   Ljungars, Anne ^b   Hallborn, Johan ^b   Ohlin, Mats ^a  

^a LUND UNIVERSITY   (Sweden)

^b BioInvent International AB   (Sweden)

Author keywords

Affinity maturation; Antibody library; Hapten; Phage display; Testosterone

Indexed keywords

ANTIBODY FRAGMENTS; ANTIBODY LIBRARIES; BINDING PROPERTIES; CONCENTRATION (COMPOSITION); IN VITRO EVOLUTION; PHAGE DISPLAYS; RANDOM MUTAGENESIS; SELECTION PROCEDURES; SELECTION STRATEGIES; SINGLE CLONE; TESTOSTERONE (T);

BINDERS; BINDING ENERGY; CLONING; CONCENTRATION (PROCESS); MUTAGENESIS;

ANTIGENS;

HAPTEN; TESTOSTERONE;

ANTIBODY AFFINITY; ANTIBODY COMBINING SITE; ANTIBODY ENGINEERING; ANTIBODY ISOLATION; ANTIBODY LIBRARY; ANTIBODY SPECIFICITY; ANTIBODY STRUCTURE; ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ARTICLE; BACTERIAL INFECTION; BACTERIOPHAGE; CLONAL VARIATION; COMPETITIVE INHIBITION; ENZYME LINKED IMMUNOSORBENT ASSAY; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

ANTIBODY AFFINITY; DIRECTED MOLECULAR EVOLUTION; HAPTENS; IMMUNOGLOBULIN FRAGMENTS; PEPTIDE LIBRARY; TESTOSTERONE;

EID: 47649099663     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzn024     Document Type: Article

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