Directed in Vitro Evolution and Crystallographic Analysis of a Peptide-binding Single Chain Antibody Fragment (scFv) with Low Picomolar Affinity

Journal of Biological Chemistry

Volumn 279, Issue 18, 2004, Pages 18870-18877

  Zahnd, Christian ^a   Spinelli, Silvia ^b   Luginbühl, Béatrice ^a   Amstutz, Patrick ^a   Cambillau, Christian ^b   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; ANTIGENS; BINDERS; DNA; OPTIMIZATION;

BINDING AFFINITY; PEPTIDE BINDERS;

BIOCHEMISTRY;

ANTIBODY; ANTIGEN; PEPTIDE;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CRYSTALLOGRAPHY; DNA SHUFFLING; EVOLUTION; IN VITRO STUDY; MOLECULAR INTERACTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RIBOSOME;

AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DIRECTED MOLECULAR EVOLUTION; ELECTROSTATICS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MODELS, MOLECULAR; MUTATION; PEPTIDE LIBRARY; PEPTIDES; PROTEIN BINDING;

MURINAE;

EID: 2442442119     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M309169200     Document Type: Article

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