A rainbow of fluoromodules: A promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes

Journal of the American Chemical Society

Volumn 130, Issue 38, 2008, Pages 12620-12621

  Özhalici Ünal, Hayriye ^a   Pow, Crystal Lee ^a   Marks, Sarah A ^a   Jesper, Lawrence D ^a   Silva, Gloria L ^a   Shank, Nathaniel I ^a   Jones, Elizabeth W ^a   Burnette III, James M ^a,b   Berget, Peter B ^a   Armitage, Bruce A ^a  

^a CARNEGIE MELLON UNIVERSITY   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYANINE DYE; DIMETHYLINDOLE RED; FLUORESCENT DYE; FLUOROGEN ACTIVATING PROTEIN; GREEN FLUORESCENT PROTEIN; MALACHITE GREEN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY CLONE K7; THIAZOLE ORANGE; UNCLASSIFIED DRUG;

ARTICLE; DRUG DESIGN; DRUG PROTEIN BINDING; DRUG SYNTHESIS; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE ANALYSIS; FLUORESCENCE CORRELATION SPECTROSCOPY; FLUOROMODULE; NEAR INFRARED SPECTROSCOPY; NONHUMAN; PHOTOACTIVATION; SCANNING LASER CONFOCAL FLUORESCENCE IMAGING;

CARBOCYANINES; COLORING AGENTS; FLOW CYTOMETRY; FLUORESCENT DYES; FUNGAL PROTEINS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; MICROSCOPY, FLUORESCENCE; PEPTIDE LIBRARY; PROTEIN BINDING; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, INFRARED; YEASTS;

EID: 52449109981     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja805042p     Document Type: Article

References (11)

1. Debler, E. W.; Kaufmann, G. F.; Meijler, M. M.; Heine, A.; Mee, J. M.; Pljevaljcic, G.; Di Bilio, A. J.; Schultz, P. G.; Millar, D. P.; Janda, K. D.; Wilson, I. A.; Gray, H. B.; Lerner, R. A. Science 2008, 319, 1232-1235.
2. Griffin, B. A.; Adams, S. R.; Tsien, R. Y. Science 1998, 281, 269-272.
3. Simeonov, A.; Matsushita, M.; Juban, E. A.; Thompson, E. H. Z.; Hoffman, T. Z.; Beuscher, A. E., IV.; Taylor, M. J.; Wirsching, P.; Rettig, W.; McCusker, J. K.; Stevens, R. C.; Millar, D. P.; Schultz, P. G.; Lerner, R. A.; Janda, K. D. Science 2000, 290, 307-313.
4. Babendure, J. R.; Adams, S. R.; Tsien, R. Y. J. Am. Chem. Soc. 2003, 125, 14716-14717.
5. Sando, S.; Narita, A.; Aoyama, Y. ChemBioChem 2007, 8, 1795-1803.
6. Sparano, B. A.; Koide, K. J. Am. Chem. Soc. 2007, 129, 4785-4794.
7. Szent-Gyorgyi, C.; Schmidt, B. F.; Creeger, Y.; Fisher, G. W.; Zakel, K. L.; Adler, S.; Fitzpatrick, J. A.; Woolford, C. A.; Yan, Q.; Vasilev, K. V.; Berget, P. B.; Bruchez, M. P.; Jarvik, J. W.; Waggoner, A. Nat. Biotechnol. 2007, 26, 235-240.
8. Feldhaus, M. J.; Siegel, R. W.; Opresko, L. K.; Coleman, J. R.; Feldhaus, J. M.; Yeung, Y. A.; Cochran, J. R.; Heinzelman, P.; Colby, D.; Swers, J.; Graff, C.; Wiley, H. S.; Wittrup, K. D. Nat. Biotechnol. 2003, 21, 163-70.
9. Constantin, T.; Silva, G. L.; Robertson, K. L.; Hamilton, T. P.; Fague, K. M.; Waggoner, A. S.; Armitage, B. A. Org. Lett. 2008, 10, 1561-1564.
10. Chao, G.; Lau, W. L.; Hackel, B. J.; Sazinsky, S. L.; Lippow, S. M.; Wittrup, K. D. Nat. Protoc. 2006, 1, 755-68.
11. Shaner, N. C.; Steinbach, P. A.; Tsien, R. Y. Nat. Methods 2005, 2, 905-909.