Detection and quantification of β2AR internalization in living cells using FAP-based biosensor technology

Journal of Biomolecular Screening

Volumn 15, Issue 6, 2010, Pages 703-709

  Fisher, Gregory W ^a   Adler, Sally A ^a   Fuhrman, Margaret H ^a   Waggoner, Alan S ^a   Bruchez, Marcel P ^a   Jarvik, Jonathan W ^a  

^a CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

cell based assays; flow cytometry; G protein coupled receptors (GPCRs); imaging; reporter gene assays

Indexed keywords

BETA 2 ADRENERGIC RECEPTOR; FLUOROGEN ACTIVATING PROTEIN; PROTEIN; UNCLASSIFIED DRUG; FLUORESCENT DYE; ISOPRENALINE; PROPRANOLOL;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BIOSENSOR; CELL SURFACE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; EMBRYO; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENETIC TRANSDUCTION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; INTERNALIZATION; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; RECOMBINANT GENE; SIGNAL TRANSDUCTION; ANIMAL; BIOASSAY; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CELL STRAIN 3T3; CELL SURVIVAL; DOSE RESPONSE; DRUG ANTAGONISM; DRUG EFFECT; DRUG POTENTIATION; ENDOCYTOSIS; GENETIC PROCEDURES; KINETICS; METABOLISM; METHODOLOGY; STAINING; SURFACE PROPERTY; TIME;

MAMMALIA;

ANIMALS; BIOLOGICAL ASSAY; BIOSENSING TECHNIQUES; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOCYTOSIS; FLUORESCENCE; FLUORESCENT DYES; HUMANS; ISOPROTERENOL; KINETICS; MICE; NIH 3T3 CELLS; PROPRANOLOL; RECEPTORS, ADRENERGIC, BETA-2; STAINING AND LABELING; SURFACE PROPERTIES; TIME FACTORS;

EID: 77955799478     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110370892     Document Type: Article

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