Comet assay

Chromosomal Alterations: Methods, Results and Importance in Human Health

Volumn 9783540714149, Issue , 2007, Pages 161-176

  Müller, Wolfgang Ulrich ^a  

^a UNIVERSITY OF DUISBURG ESSEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84881337728     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-3-540-71414-9_10     Document Type: Chapter

References (69)

1. Alapetite C, Thirion P, de la Rochefordière A, Cosset J-M, Moustacchi E (1999) Analysis by alkaline comet assay of cancer patients with severe reactions to radiotherapy: Defective rejoining of radioinduced DNA strand breaks in lymphocytes of breast cancer patients. Int J Cancer 83:83-90
2. Anderson D, Yu TW, McGregor DB (1998) Comet assay responses as indicators of carcinogen exposure. Mutagenesis 13:539-555
3. Arlett CF, Lowe JE, Harcourt SA, Waugh A P W, Cole J, Roza L, Diffey BL, Mori T, Nikaido O, Green M H L (1993) Hypersensitivity of human lymphocytes to UV-B and solar irradiation. Cancer Res 53:609-614
4. Böcker W, Bauch T, Müller W-U, Streffer C (1997) Image analysis of comet assay measurements. Int J Radiat Biol 72:449-460
5. Brendler-Schwaab S, Hartmann A, Pfuhler S, Speit G (2005) The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis 20:245-254
6. Collins A, Dusinska M, Franklin M, Somorovska M, Petrovska H, Duthie S, Fillion L, Panayiotidis M, Raslova K, Vaughan N (1997a) Comet assay in human biomonitoring studies: reliability, validation, and applications. Environ Mol Mutagen 30:139-146
7. Collins AR (2004) The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 26:249-261
8. Collins AR, Dobson VL, Dusinska M, Kennedy G, Stetina R (1997b) The comet assay: what can it really tell us? Mutat Res 375:183-193
9. Cotelle S, Ferard JF (1999) Comet assay in genetic ecotoxicology: a review. Environ Mol Mutagen 34:246-255
10. Faust F, Kassie F, Knasmuller S, Boedecker RH, Mann M, Mersch-Sundermann V (2004) The use of the alkaline comet assay with lymphocytes in human biomonitoring studies. Mutat Res 566:209-229
11. Frenzilli G, Lori A, Panasiuk G, Ferdeghini M, Barale R (1998) Comet assay on children's leukocytes 8 years after the Chernobyl disaster. Mutat Res 415:151-158
12. Garcia O, Mandina T (2005) DNA damage evaluated by the comet assay in lymphocytes of children with 137Cs internal contamination caused by the Chernobyl accident. Mutat Res 565:191-197
13. Garcia O, Mandina T, Lamadrid AI, Diaz A, Remigio A, Gonzalez Y, Piloto J, Gonzalez JE, Alvarez A (2004) Sensitivity and variability of visual scoring in the comet assay. Results of an inter-laboratory scoring exercise with the use of silver staining. Mutat Res 556:25-34
14. Gedik CM, Ewen S W B, Collins AR (1992) Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int J Radiat Biol 62:313-320
15. Green M H L, Lowe JE, Harcourt SA, Akinluyi P, Rowe T, Cole J, Anstey AV, Arlett CF (1992) UV-C sensitivity of unstimulated and stimulated human lymphocytes from normal and xeroderma pigmentosum donors in the comet assay: A potential diagnostic technique. Mutat Res 273:137-144
16. Hartmann A, Plappert U, Raddatz K, Grünert-Fuchs M, Speit G (1994) Does physical activity induce DNA damage? Mutagenesis 9:269-272
17. Hartmann A, Pfuhler S, Dennog C, Germadnik D, Pilger A, Speit G (1998) Exercise-induced DNA effects in human leukocytes are not accompanied by increased formation of 8-hydroxy-2'-deoxyguanosine or induction of micronuclei. Free Radic Biol Med 24:245-251
18. Hartmann A, Agurell E, Beevers C, Brendler-Schwaab S, Burlinson B, Clay P, Collins A, Smith A, Speit G, Thybaud V, Tice RR (2003) Recommendations for conducting the in vivo alkaline comet assay. 4th international comet assay workshop. Mutagenesis 18:45-51
19. Hellman B, Friis L, Vaghef H, Edling C (1999) Alkaline single cell gel electrophoresis and human biomonitoring for genotoxicity: a study on subjects with residential exposure to radon. Mutat Res 442:121-132
20. Hoffmann H, Hogel J, Speit G (2005) The effect of smoking on DNA effects in the comet assay: a meta-analysis. Mutagenesis 20:455-466
21. ICRP (1999) Genetic susceptibility to cancer. ICRP publication 79. Pergamon, Oxford
22. Kassie F, Parzefall W, Knasmuller S (2000) Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat Res 463:13-31
23. Kruszewski M, Wojewodzka M, Iwanenko T, Collins AR, Szumiel I (1998) Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation. II. Base damage. Mutat Res 416:37-57
24. Kumaravel TS, Jha AN (2006) Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutat Res 605:7-16
25. Ladeira MS, Rodrigues MA, Freire-Maia DV, Salvadori DM (2005) Use of Comet assay to assess DNA damage in patients infected by Helicobacter pylori: comparisons between visual and image analyses. Mutat Res 586:76-86
26. Malyapa RS, Bi C, Ahern EW, Roti Roti JL (1998) Detection of DNA damage by the alkaline comet assay after exposure to low-dose gamma radiation. Radiat Res 149:396-400
27. McKelvey VJ, Tice RR, Singh NP (1990) A technique for measuring DNA single strand breaks and alkali labile sites in individual cells. Br J Cancer 62:529-530
28. McKelvey-Martin VJ, Ho ET, McKeown SR, Johnston SR, McCarthy PJ, Rajab NF, Downes CS (1998) Emerging applications of the single cell gel electrophoresis (comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization comets for the identification of damaged and repaired DNA sequences in individual cells. Mutagenesis 13:1-8
29. McKenna DJ, Gallus M, McKeown SR, Downes CS, McKelvey-Martin VJ (2003) Modification of the alkaline Comet assay to allow simultaneous evaluation of mitomycin C-induced DNA cross-link damage and repair of specific DNA sequences in RT4 cells. DNA Repair (Amst) 2:879-890
30. McKeown SR, Robson T, Price ME, Ho ET, Hirst DG, McKelvey-Martin VJ (2003) Potential use of the alkaline comet assay as a predictor of bladder tumour response to radiation. Br J Cancer 89:2264-2270
31. Mitchelmore CL, Chipman JK (1998) DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat Res 399:135-147
32. Moller P (2005) Genotoxicity of environmental agents assessed by the alkaline comet assay. Basic Clin Pharmacol Toxicol 96(Suppl 1):1-42
33. Moller P (2006a) Assessment of reference values for DNA damage detected by the comet assay in human blood cell DNA. Mutat Res 612:84-104
34. Moller P (2006b) The alkaline comet assay: towards validation in biomonitoring of DNA damaging exposures. Basic Clin Pharmacol Toxicol 98:336-345
35. Moller P, Knudsen LE, Loft S, Wallin H (2000) The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors. Cancer Epidemiol Biomarkers Prev 9:1005-1015
36. Müller W-U, Bauch T, Streffer C, Niedereichholz F, Böcker W (1994) Comet assay studies of radiation-induced DNA damage and repair in various tumour cell lines. Int J Radiat Biol 65:315-319
37. Müller W-U, Bauch T, Streffer C (1999) The application of comet and micronucleus assay for the assessment of individual radiation risk. In: Baumstark-Khan C, Kozubek S, Horneck G (eds): Fundamentals for the assessment of risks from environmental radiation. Kluwer, Dordrecht, pp 127-134
38. Müller W-U, Bauch T, Stüben G, Sack H, Streffer C (2001) Radiation sensitivity of lymphocytes of healthy individuals and cancer patients as measured by the comet assay. Radiat Environ Biophys 40:83-89
39. Müller W-U, Bauch T, Streffer C, von Mallek D (2002) Does radiotherapy affect the outcome of the comet assay? Br J Radiol 75:608-614
40. Müller W-U, Ciborovius J, Bauch T, Johannes C, Schunck C, Mallek U, Böcker W, Obe G, Streffer C (2004) Analysis of the action of the restriction endonuclease AluI using three different comet assay protocols. Strahlenther Onkol 180:655-664
41. NRPB (1999) Genetic heterogeneity in the population and its implications for radiation risk. National Radiological Protection Board, Chilton
42. Olive PL (1999) DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int J Radiat Biol 75:395-405
43. Olive PL (2002) The comet assay. An overview of techniques. Methods Mol Biol 203:179-194
44. Olive PL, Durand RE (1992) Detection of hypoxic cells in a murine tumor with the use of the comet assay. J Nat Canc Inst 84:707-711
45. Olive PL, Banáth J, Durand RE (1990) Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay. Radiat Res 122:86-94
46. Olive PL, Wlodek D, Banáth JP (1991) DNA double-strand breaks measured in individual cells subjected to gel electrophoresis. Cancer Res 51:4671-4676
47. Olive PL, Wlodek D, Durand RE, Banáth JP (1992) Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp Cell Res 198:259-267
48. Olive PL, Durand RE, Le Riche J, Olivotto IA, Jackson SM (1993a) Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers. Cancer Res 53:733-736
49. Olive PL, Frazer G, Banáth JP (1993b) Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat Res 136:130-136
50. Ostling O, Johanson KJ (1984) Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123:291-298
51. Pandey AK, Bajpayee M, Parmar D, Rastogi SK, Mathur N, Seth PK, Dhawan A (2006) DNA damage in lymphocytes of Indian rickshaw pullers as measured by the alkaline comet assay. Environ Mol Mutagen 47:25-30
52. Pfuhler S, Wolf HU (1996) Detection of DNA-crosslinking agents with the alkaline comet assay. Environ Mol Mutagen 27:196-201
53. Plappert UG, Stocker B, Fender H, Fliedner TM (1997) Changes in the repair capacity of blood cells as a biomarker for chronic low-dose exposure to ionizing radiation. Environ Mol Mutagen 30:153-160
54. Rajeswari N, Ahuja YR, Malini U, Chandrashekar S, Balakrishna N, Rao KV, Khar A (2000) Risk assessment in first degree female relatives of breast cancer patients using the alkaline comet assay. Carcinogenesis 21:557-561
55. Rojas E, Lopez MC, Valverde M (1999) Single cell gel electrophoresis assay: methodology and applications. J Chromatogr B Biomed Sci Appl 722:225-254
56. Santos SJ, Singh NP, Natarajan AT (1997) Fluorescence in situ hybridization with comets. Exp Cell Res 232:407-411
57. Sasaki YF, Sekihashi K, Izumiyama F, Nishidate E, Saga A, Ishida K, Tsuda S (2000) The comet assay with multiple mouse organs: comparison of comet assay results and carcinogenicity with 208 chemicals selected from the IARC monographs and US NTP Carcinogenicity Database. Crit Rev Toxicol 30:629-799
58. Schabath MB, Spitz MR, Grossman HB, Zhang K, Dinney CP, Zheng PJ, Wu X (2003) Genetic instability in bladder cancer assessed by the comet assay. J Natl Cancer Inst 95:540-547
59. Shao L, Lin J, Huang M, Ajani JA, Wu X (2005) Predictors of esophageal cancer risk: assessment of susceptibility to DNA damage using comet assay. Genes Chromosomes Cancer 44:415-422
60. Sigurdson AJ, Hauptmann M, Alexander BH, Doody MM, Thomas CB, Struewing JP, Jones IM (2005) DNA damage among thyroid cancer and multiple cancer cases, controls, and longlived individuals. Mutat Res 586:173-188
61. Singh NP, Graham MM, Singh V, Khan A (1995) Induction of DNA single-strand breaks in human lymphocytes by low doses of gamma-rays. Int J Radiat Biol 68:563-569
62. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191
63. Singh NP, Stephens RE, Schneider EL (1994) Modifications of alkaline microgel electrophoresis for sensitive detection of DNA damage. Int J Radiat Biol 66:23-28
64. Smith TR, Miller MS, Lohman KK, Case LD, Hu JJ (2003) DNA damage and breast cancer risk. Carcinogenesis 24:883-889
65. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206-221
66. Trenz K, Schutz P, Speit G (2005) Radiosensitivity of lymphoblastoid cell lines with a heterozygous BRCA1 mutation is not detected by the comet assay and pulsed field gel electrophoresis. Mutagenesis 20:131-137
67. Tsai K, Hsu TG, Hsu KM, Cheng H, Liu TY, Hsu CF, Kong CW (2001) Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise. Free Radic Biol Med 31:1465-1472
68. Wojewodzka M, Kruszewski M, Iwanenko T, Collins AR, Szumiel I (1998) Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation. I. Strand breakage. Mutat Res 416:21-35
69. Zhang H, Buchholz TA, Hancock D, Spitz MR, Wu X (2000) Gamma-radiation-induced single cell DNA damage as a measure of susceptibility to lung cancer: a preliminary report. Int J Oncol 17:399-404