Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation I. Strand breakage

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 416, Issue 1-2, 1998, Pages 21-35

  Wojewódzka, Maria ^a   Kruszewski, Marcin ^a   Iwaneñko, Teresa ^a   Collins, Andrew R ^b   Szumiel, Irena ^a  

^a INSTITUTE OF NUCLEAR CHEMISTRY AND TECHNOLOGY   (Poland)

^b ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Chronic low dose irradiation; Comet assay; Human mononuclear blood cell; Occupational risk

Indexed keywords

ADULT; AGED; ARTICLE; DNA DAMAGE; DNA STRAND BREAKAGE; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; OCCUPATIONAL HAZARD; PRIORITY JOURNAL; RADIATION INJURY; WORKPLACE;

ADULT; AGED; CASE-CONTROL STUDIES; DNA; DNA DAMAGE; FEMALE; FILM DOSIMETRY; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MIDDLE AGED; NUCLEAR ENERGY; OCCUPATIONAL EXPOSURE; RADIATION MONITORING; RISK FACTORS;

EID: 0344301939     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(98)00073-4     Document Type: Article

References (18)

1. N.P. Singh, R.E. Stephens, E.L. Schneider, Modifications of alkaline microgel electrophoresis for sensitive detection of DNA damage, Int. J. Radiat. Biol. 66 (1994) 23-28.
2. C.S. Aaron, D.M. Zimmer, P.R. Harbach, R.L. Yu, End-points for biomonitoring: assay sensitivity/selectivity, Environ. Health Perspect. 104 (1996) 521-525.
3. N.P. Singh, M.T. McCoy, R.R. Tice, E.L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res. 175 (1988) 184-191.
4. M. Kruszewski, M.H. Green, J.E. Lowe, I. Szumiel, DNA strand breakage, cytotoxicity and mutagenicity of hydrogen peroxide treatment at 4°C and 37°C in L5178Y sublines, Mutat. Res. 308 (1994) 233-241.
5. J.H. Zar, Biostatistical Analysis, Prentice-Hall, Englewood Cliffs, NJ, 1984.
6. S. Asami, T. Hirano, R. Yamaguchi, Y. Tomioka, H. Itoh, H. Kasai, Increase of a type of oxidative DNA damage, 8-hydroxyguanine, and its repair activity in human leukocytes by cigarette smoking, Cancer Res. 56 (1996) 2546-2549.
7. W.W. Au, G.S. Wilkinson, S.K. Tyring, M.S. Legator, R. el Zein, L. Hallberg, M.Y. Heo, Monitoring populations for DNA repair deficiency and for cancer susceptibility, Environ. Health Perspect. 104 (1996) 579-584.
8. M. Kruszewski, M. Wojewódzka, T. Iwaneñko, A.R. Collins, I. Szumiel, Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation: II. Base damage, Mutat. Res. (1998), submitted.
9. M.C. Chicca, C. Nesti, M. Muzzoli, P. Pasetti, S. Pinamonti, Correlation between age and DNA damage detected by FADU in human peripheral blood lymphocytes, Mutat. Res. 316 (1996) 201-208.
10. H. Thierens, A. Vral, L. De Ridder, A cytogenetic study of radiological workers: effect of age, smoking and radiation burden on the micronucleus frequency, Mutat. Res. 360 (1996) 75-82.
11. L. Migliore, M. Parrini, I. Sbrana, C. Biagini, A. Battaglia, N. Loprieno, Micronucleated lymphocytes in people occupationally exposed to potential environmental contaminants: the age effect, Mutat. Res. 256 (1991) 13-20.
12. S. Ban, J.B. Cologne, S. Fujita, A.A. Awa, Radiosensitivity of atomic bomb survivors as determined with a micronucleus assay, Radiat. Res. 134 (1993) 170-178.
13. M. Fenech, A.A. Morley, Measurement of micronuclei in lymphocytes, Mutat. Res. 147 (1985) 29-36.
14. D.K. Monteith, J. Vanstone, Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the detection of DNA damage, Mutat. Res. 345 (1995) 97-103.
15. M. Klaude, S. Eriksson, J. Nygren, G. Ahnstrom, The comet assay: mechanisms and technical considerations, Mutat. Res. 363 (1996) 89-96.
16. A. Collins, M. Dusinska, M. Franklin, M. Somorovska, H. Petrovska, S. Duthie, L. Fillion, M. Panayiotidis, K. Raslova, N. Vaughan, Comet assay in human biomonitoring studies: reliability, validation, and applications, Environ. Mol. Mutagen. 30 (1997) 139-146.
17. S.M. Clutton, K.M. Townsend, C. Walker, J.D. Ansell, E.G. Wright, Radiation-induced genomic instability and persisting oxidative stress in primary bone marrow cultures, Carcinogenesis 17 (1996) 1633-1639.
18. U.G. Plappert, B. Stocker, H. Fender, T.M. Fliedner, Changes in the repair capacity of blood cells as biomarker for chronic low-dose exposure to ionizing radiation, Environ. Mol. Mutagen. 30 (1997) 153-160.