Comet assay on children's leukocytes 8 years after the Chernobyl disaster

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 415, Issue 1-2, 1998, Pages 151-158

  Frenzilli, G ^a   Lori, A ^a   Panasiuk, G ^b   Ferdeghini, M ^a   Barale, R ^a  

^a UNIVERSITY OF PISA   (Italy)

^b Radiological Dispensary   (Belarus)

Author keywords

Adaptive response; Bleomycin; DNA damage; Human leukocyte; Single cell gel electrophoresis

Indexed keywords

BLEOMYCIN; CESIUM 137; SINGLE STRANDED DNA BINDING PROTEIN;

ADAPTATION; ADOLESCENT; ARTICLE; CHERNOBYL ACCIDENT; CLINICAL ARTICLE; CONTROLLED STUDY; DNA DAMAGE; FEMALE; GEL ELECTROPHORESIS; GENOTOXICITY; HUMAN; HUMAN CELL; LEUKOCYTE; MALE; MUTAGEN TESTING; PRIORITY JOURNAL; RADIATION INJURY; SCHOOL CHILD; THYROID CANCER;

ACCIDENTS, RADIATION; ADOLESCENT; BIOLOGICAL ASSAY; BLEOMYCIN; CHILD; DISASTERS; DNA DAMAGE; FEMALE; HUMANS; LEUKOCYTES; MALE; POWER PLANTS; THYROID NEOPLASMS; UKRAINE;

EID: 0031844105     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(98)00060-6     Document Type: Article

References (22)

1. G. Olivieri, J. Bodycote, S. Wolff, Adaptive response in human lymphocytes to low concentrations of radioactive thymidine, Science 22 (1984) 594-597.
2. S. Wolff, V. Afzal, J.K. Wiencke, G. Olivieri, A. Michaeli, Human lymphocytes exposed to low doses of ionizing radiations become refractory to high doses of radiation as well as to chemical mutagens that induce double-strand breaks in DNA, Int. J. Radiat. Biol. 53 (1988) 39-48.
3. K. Sankaranarayanan, A.v. Duyn, M.J. Looos, A.T. Natarajan, Adaptive response of human lymphocytes to low-level radiation from radioisotopes or X-rays, Mutat. Res. 211 (1989) 7-12.
4. 3H}thymidine and the inability to measure human lymphocyte proliferation by incorporated radioactivity, Proc. Natl. Acad. Sci. U.S.A. 83 (1986) 4749-4753.
5. B.J.S. Sanderson, A.A. Morley, Exposure of human lymphocytes to ionizing radiation reduces mutagenesis by subsequent ionizing radiation, Mutat. Res. 164 (1986) 347-351.
6. L. Cai, S.-Z. Liu, Induction of cytogenetic adaptive response of somatic and germ cells in vivo and in vitro by low-dose X-irradiation, Int. J. Radiat. Biol. 58 (1990) 187-194.
7. o human lymphocytes conditioned with hydrogen peroxide or low-dose X-rays, Mutat. Res. 311 (1994) 157-163.
8. B. Tedeschi, D. Caporossi, P. Vernole, L. Padovani, F. Mauro, Do human lymphocytes exposed to the fallout of the Chernobyl accident exhibit an adaptive response? III. Challenge with bleomycin in lymphocytes from children hit by the initial acute dose of ionizing radiation, Mutat. Res. 354 (1996) 77-80.
9. D.W. Fairbairn, P.L. Olive, K.L. O'Neill, The comet assay: a comprehensive review, Mutat. Res. 339 (1995) 37-59.
10. R.R. Tice, The single cell gel/Comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells, in: D.H. Phillips, S. Venit (Eds.), Environmental Mutagenesis, Chap. 16, Bios Scientific Publishers, Oxford, UK (1995) pp. 315-338.
11. M.H.L. Green, J.E. Lowe, S.A. Harcourt, P. Akinluyi, T. Rowe, J. Cole, A.V. Anstey, C.F. Arlett, UV-C sensitivity of unstimulated and stimulated human lymphocytes from normal and xeroderma pigmentosum donors in the comet assay: a potential diagnostic technique, Mutat. Res. 273 (1992) 137-144.
12. N.P. Singh, M.T. McCoy, R.R. Tice, E.L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res. 175 (1988) 184-191.
13. L.A. Videla, T. Sir, C. Wolff, Increased lipid peroxidation in hyperthyroid patients: suppression by propylthiouracil treatment, Free Radic. Res. Commun. 5 (1) (1988) 1-10.
14. M. Sugawara, T. Kita, E.D. Lee, J. Takamatsu, G.A. Hagen, K. Kuma, G.A. Medeiros-Neto, Deficiency of superoxide dismutase in endemic goiter tissue, J. Clin. Endocrinol. Metab. 67 (6) (1988) 1156-1161.
15. K. Asayama, K. Dobashi, H. Hayashibe, Y. Megata, K. Kato, Lipid peroxidation and free radical scavengers in thyroid dysfunction in the rat: a possible mechanism of injury to heart and skeletal muscle in hyperthyroidism, Endocrinology 121 (6) (1987) 2112-2118.
16. N.P. Singh, D.B. Danner, R.R. Tice, L. Brant, E.L. Schneider, DNA damage and repair with age in individual human lymphocytes, Mutat. Res. 237 (1990) 123-130.
17. I. Emerit, M. Quastel, J. Goldsmith, L. Merkin, A. Levy, L. Cernjavski, A. Alaoui-Youssefi, A. Pogossian, E. Riklis, Clastogenic factors in the plasma of children exposed at Chernobyl, Mutat. Res. 373 (1997) 47-54.
18. T. Ikushima, H. Aritomi, J. Morisita, Radioadaptive response: efficient repair of radiation-induced DNA damage in adapted cells, Mutat. Res. 358 (1996) 193-198.
19. L.F. Porvik, M.J. Finley Austin, Genotoxicity of bleomycin, Mutat. Res. 257 (1991) 127-143.
20. A. Bosi, G. Olivieri, Variability of the adaptive response to ionizing radiations in humans, Mutat. Res. 211 (1989) 13-17.
21. A. Hartmann, U. Plappert, K. Raddatz, M. Grunert-Fuchs, G. Speit, Does physical activity induce DNA damage?, Mutagenesis 9 (3) (1994) 269-272.
22. A.R. Collins, M. Ai-guo, S.J. Duthie, The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells, Mutat. Res. 336 (1995) 69-77.