Detection of DNA damage by the alkaline comet assay after exposure to low-dose gamma radiation

Radiation Research

Volumn 149, Issue 4, 1998, Pages 396-400

  Malyapa, Robert S ^a   Bi, Chen ^a   Ahern, Eric W ^a   Roti Roti, Joseph L ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA DAMAGE; DNA DETERMINATION; FIBROBLAST; GAMMA IRRADIATION; IONIZING RADIATION; LOW ENERGY RADIATION; LYMPHOCYTE; MOUSE; NONHUMAN; PRIORITY JOURNAL; RADIATION EXPOSURE; RAT;

ANIMALS; CELL LINE; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, RADIATION; ELECTROPHORESIS, AGAR GEL; GAMMA RAYS; HYDROGEN-ION CONCENTRATION; LYMPHOCYTES; MICE; MICE, INBRED C3H; RATS; RATS, SPRAGUE-DAWLEY; TEMPERATURE;

ANIMALIA;

EID: 0031922151     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.2307/3579703     Document Type: Article

References (11)

1. O. Östling and K. J. Johanson, Microelectrophoretic study of radiation-induced DNA damages in individual cells. Biochem. Biophys. Res. Commun. 123, 291-298 (1984).
2. N. P. Singh, M. T. McCoy, R. R. Tice and E. L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175, 184-191 (1988).
3. P. L. Olive, D. Wlodek, R. E. Durand and J. P. Banáth, Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res. 198, 259-267 (1992).
4. N. P. Singh, R. E. Stephens and E. L. Schneider, Modification of alkaline microgel electrophoresis for sensitive detection of DNA damage. Int. J. Radiat. Biol. 66, 23-28 (1994).
5. C. R. H. Kent, J. J. Eady, G. M. Ross and G. G. Steel, The comet moment as a measure of DNA damage in the comet assay. Int. J. Radiat. Biol. 67, 655-660 (1995).
6. D. W. Fairbairn, P. L. Olive and K. L. O'Neill, The cornet assay: A comprehensive review. Mutat. Res. 339, 37-59 (1995).
7. M. Klaude, S. Eriksson, J. Nygren and G. Ahnström, The comet assay: Mechanisms and technical considerations. Mutat. Res. 363, 89-96 (1996).
8. U. Plappert, K. Raddatz, S. Roth and T. M. Fliedner, DNA-damage detection in man after radiation exposure - the comet assay - its possible application for human biomonitoring. Stem Cells 13 (Suppl. 1), 215-222 (1995).
9. R. S. Malyapa, E. W. Ahern, W. L. Straube, E. G. Moros, W. F. Pickard and J. L. Roti Roti, Measurement of DNA damage following exposure to 2450 MHz electromagnetic radiation. Radiat. Res. 148, 608-617 (1997).
10. R. R. Tice, The single cell gel/comet assay: A microgel electrophoretic technique for the detection of DNA damage and repair in individual cells. In Environmental Mutagenesis (D. H. Phillipps and S. Venitt, Eds.), pp. 315-339. Bios Scientific Publishers, Oxford, 1995.
11. Vijayalaxmi, R. R. Tice and G. H. S. Strauss, Assessment of radiation-induced DNA damage in human blood lymphocytes using the single-cell gel electrophoresis technique. Mutat. Res. 271, 243-252 (1992).