DNA damage in lymphocytes of Indian rickshaw pullers as measured by the alkaline comet assay

Environmental and Molecular Mutagenesis

Volumn 47, Issue 1, 2006, Pages 25-30

  Pandey, Alok Kumar ^a   Bajpayee, Mahima ^a   Parmar, Devendra ^a   Rastogi, Subodh K ^a   Mathur, Neeraj ^a   Seth, Prahlad K ^a   Dhawan, Alok ^a  

^a CSIR INDIAN INSTITUTE OF TOXICOLOGY RESEARCH   (India)

Author keywords

Comet assay; DNA damage; Indian population; Peripheral blood lymphocytes; Physical activity; Rickshaw pullers

Indexed keywords

CELL DNA; GENOMIC DNA; REACTIVE OXYGEN METABOLITE;

ADULT; AIR POLLUTANT; ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; ENVIRONMENTAL EXPOSURE; HEALTH BEHAVIOR; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; INDIA; LYMPHOCYTE; MALE; NORMAL HUMAN; OCCUPATIONAL EXPOSURE; PHYSICAL ACTIVITY; SOCIAL STATUS; STATISTICAL SIGNIFICANCE; URBAN POPULATION;

ADULT; AIR POLLUTANTS; COMET ASSAY; DNA DAMAGE; EXERCISE; HUMANS; INDIA; LYMPHOCYTES; MALE; MIDDLE AGED; OCCUPATIONAL EXPOSURE; OXIDATIVE STRESS; TRANSPORTATION;

EID: 32544448660     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/em.20161     Document Type: Article

References (42)

1. Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, Merlo F, Natarajan AT, Norppa H, Shuker DE, Tice R, Waters MD, Aitio A. 2000. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutat Res 463:111-172.
2. Alessio HM. 1993. Exercise-induced oxidative stress. Med Sci Sports Exerc 25:218-224.
3. Asami S, Hirano T, Yamaguchi R, Itoh H, Kasai H. 1998. Reduction of 8-hydroxyguanine in human leukocyte DNA by physical exercise. Free Radic Res 29:581-584.
4. Awara WM, El-Nabi SH, El-Gohary M. 1998. Assessment of vinyl chloride-induced DNA damage in lymphocytes of plastic industry workers using a single-cell gel electrophoresis technique. Toxicology 128:9-16.
5. Bajpayee M, Dhawan A, Parmar D, Pandey AK, Mathur N, Seth PK. 2002. Gender related differences in basal DNA damage in lymphocytes of a healthy Indian population using alkaline Comet assay. Mutat Res 520:83-91.
6. Bajpayee M, Pandey AK, Parmar D, Mathur N, Seth PK, Dhawan A. 2005. Comet assay responses in human lymphocytes are not influenced by the menstrual cycle: a study in healthy Indian females. Mutat Res 565:163-172.
7. Betti C, Davini T, Giannessi L, Loprieno N, Barale R. 1994. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat Res 307:323-333.
8. Betti C, Davini T, Giannessi L, Loprieno N, Barale R. 1995. Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects. Mutat Res 343:201-207.
9. Chaudhary AK, Nokubo M, Reddy GR, Yeola SN, Morrow JD, Blair IA, Marnett LJ. 1994. Detection of endogenous malondialdehydedeoxyguanosine adducts in human liver. Science 265:1580-1582.
10. Collins A, Dusinska M, Franklin M, Somorovska M, Petrovska H, Duthie S, Fillion L, Panayiotidis M, Raslova K, Vaughan N. 1997. Comet assay in human biomonitoring studies: reliability, validation and applications. Environ Mol Mutagen 30:139-146.
11. Davies KJ, Quintanilha AT, Brooks GA, Packer L. 1982. Free radicals and tissue damage produced by exercise. Biochem Biophy Res Commun 107:1198-1205.
12. Demir S, Turgut G, Yurtseven O. Asian D, Gene O. 2001. Effect of exercise on lipid peroxidation. Acta Medica 44:41-42.
13. Dey A. Parmar D, Dhawan A, Dash D, Seth PK. 2002. Cytochrome P450 2E1(CYP 2E1) dependent catalytic activity and lipid peroxidation in rat blood lymphocytes. Life Sci 71:2509-2519.
14. Dhawan A, Mathur N. Seth PK. 2001. The effect of smoking and eating habits on DNA damage in Indian population as measured in the comet assay. Mutat Res 474:121-128.
15. Felley-Bosco E. 1998. Role of nitric oxide in genotoxicity: implication for carcinogenesis. Cancer Metastasis Rev 17:25-37.
16. Garaj-Vrhovac V, Zeljezic D. 2002. Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, micronucleus assay and Comet assay. J Appl Toxicol 22:249-55.
17. Hartmann A, Plappert U, Raddatz K, Grunert-Fuchs M, Speit G. 1994. Does physical activity induce DNA damage? Mutagenesis 2:269-272.
18. Hartmann A, Herkommer K, Gluck M, Speit G. 1995a. DNA damaging effect of cyclophosphamide on human blood cells in vivo and in vitro studied with the single cell gel test (Comet assay). Environ Mol Mutagen 25:180-187.
19. Hartmann A, Niess AM, Grunert-Fuchs M, Poch B, Speit G. 1995b. Vitamin E prevents exercise-induced DNA damage. Mutat Res 346:195-202.
20. Hartmann A, Pfuhler S, Dennog C, Germadnik D, Pilger A, Speit G. 1998. Exercise-induced DNA effects in human leukocytes are not accompanied by increased formation of 8-hydroxy-2′-deoxyguanosine or induction of micronuclei. Free Radic Biol Med 24:245-251.
21. Huang HY, Helzlsouer KJ, Appel LJ. 2000. The effects of vitamin C and vitamin E on oxidative DNA damage: results from a randomized controlled trial. Cancer Epidemiol Biomarkers Prev 9:647-652.
22. Ji LL. 1995. Oxidative stress during exercise: implication of antioxidant nutrients. Free Radic Biol Med 18:1079-1086.
23. Kasai H, Iwamoto-Tanaka N, Miyamoto T, Kawanami K, Kawanami S, Kido R, Ikeda M. 2001. Life style and urinary 8-hydroxydeoxy-guanosine, a marker of oxidative DNA damage: effects of exercise, working conditions, meat intake, body mass index and smoking. Jpn J Cancer Res 92:9-15.
24. Kassie F, Parzefall W, Knasmuller S. 2000. Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat Res 463:13-31.
25. Mars M, Govender S, Weston A, Naicker V, Chuturgoon A. 1998. High intensity exercise: a cause of lymphocyte apoptosis? Biochem Biophys Res Commun 249:366-370.
26. Niess AM, Baumann M, Roecker K, Horstmann T, Mayer F, Dickhuth HH. 1998. Effects of intensive endurance exercise on DNA damage in leucocytes. J Sports Med Phys Fitness 38:111-115.
27. Pandey AK, Bajpayee M, Parmar D, Rastogi SK, Mathur N, Seth PK, Dhawan A. 2005. DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay. Environ Mol Mutagen 45:435-441.
28. Phillips HJ. 1973. Dye exclusion tests for cell viability. In: Kruse PF, Patterson MJ, editors. Tissue Culture: Methods and Applications. New York: Academic Press, pp 406-408.
29. Piperakis SM, Visvardis EE, Sagnou M, Tassiou AM. 1998. Effects of smoking and aging on oxidative DNA damage of human lymphocytes. Carcinogenesis 19:695-698.
30. Piperakis SM, Petrakou E, Tsilimigaki S. 2000. Effect of air pollution and smoking on DNA damage of human lymphocyte. Environ Mol Mutagen 36:243-249.
31. Poulsen HE, Loft S, Vistisen K, 1996. Extreme exercise and oxidative DNA modification. J Sports Sci 14:343-346.
32. Poulsen HE, Weismann A, Loft S. 1999. Methods to detect DNA damage by free radicals: relation to exercise. Proc Nutr Soc 58:1007-1014.
33. Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191.
34. Singh NP, Danner DB, Tice RR. Brant L, Schneider EL. 1990. DNA damage and repair with age in individual human lymphocytes. Mutat Res 237:123-130.
35. Singh NP, Danner DB, Tice RR, Pearson JD, Brant LJ, Morrell CH. Schneider EL. 1991. Basal DNA damage in individual human lymphocytes with age. Mutat Res 256:1-6.
36. Sjodin B, Hellsten Westing Y, Apple FS. 1990. Biochemical mechanisms for oxygen free radical formation during exercise. Sports Med 10:236-254.
37. Tice RR. 1995. Applications of the single cell gel assay to environmental biomonitoring for genotoxic pollutants. In: Butterworth BE, Corkum LD, Guzmán-Rincón J, editors. Biomonitors and Biomarkers as Indicators of Environmental Change. New York: Plenum, pp 69-79.
38. Tice RR, Andrews PW, Singh NP. 1990. The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair. Basic Life Sci 53:291-301.
39. Tsai K, Hsu TG, Hsu KM, Cheng H, Liu TY, Hsu CF, Kong CW. 2001. Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise. Free Radic Biol Med 31:1465-1472.
40. Undeger U, Zorlu AF, Basaran N. 1999. Use of the alkaline comet assay to monitor DNA damage in technicians exposed to low-dose radiation. J Occup Environ Med 41:693-698.
41. Wiseman H, Halliwell B. 1996. Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer. Biochem J 313:17-29.
42. World Resources Institute. 1998. Health effect of air pollution: rising energy use (Chapter 2). In World Resources 1998-99, Part I: Environmental Changes and Human Health (http://population. wri.org/pubs_content_text.cfm? contentID=1519).