Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation II. Base damage

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 416, Issue 1-2, 1998, Pages 37-57

  Kruszewski, Marcin ^a   Wojewódzka, Maria ^a   Iwanenko, Teresa ^a   Collins, Andrew R ^b   Szumiel, Irena ^a  

^a INSTITUTE OF NUCLEAR CHEMISTRY AND TECHNOLOGY   (Poland)

^b ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Base damage; Comet assay; Human mononuclear blood cell; Occupational risk

Indexed keywords

ENDONUCLEASE;

ADULT; AGED; ARTICLE; DNA DAMAGE; DNA STRAND BREAKAGE; FEMALE; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; MONITORING; MONONUCLEAR CELL; OCCUPATIONAL HAZARD; PRIORITY JOURNAL; RADIATION INJURY; WORKPLACE;

ADULT; AGED; CASE-CONTROL STUDIES; DEOXYRIBONUCLEASE (PYRIMIDINE DIMER); DNA; DNA DAMAGE; DNA-FORMAMIDOPYRIMIDINE GLYCOSYLASE; ENDODEOXYRIBONUCLEASES; ESCHERICHIA COLI PROTEINS; FEMALE; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MIDDLE AGED; N-GLYCOSYL HYDROLASES; OCCUPATIONAL EXPOSURE; RADIATION MONITORING; RISK FACTORS;

EID: 0344222204     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(98)00074-6     Document Type: Article

References (16)

1. M. Wojewodzka, M. Kruszewski, T. Iwanenko, A.R. Collins, I. Szumiel, Application of the comet assay for monitoring DNA damage in workers exposed to chronic low dose irradiation. I. Strand breakage, Mutat. Res. 416 (1998) 21-35.
2. M. Klaude, S. Eriksson, J. Nygren, G. Ahnstrom, The comet assay: mechanisms and technical considerations, Mutat. Res. 363 (1996) 89-96.
3. A.R. Collins, S.J. Duthie, V.L. Dobson, Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA, Carcinogenesis 14 (1993) 1733-1735.
4. N.P. Singh, M.T. McCoy, R.R. Tice, E.L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res. 175 (1988) 184-191.
5. M. Kruszewski, M.H. Green, J.E. Lowe, I. Szumiel, DNA strand breakage, cytotoxicity and mutagenicity of hydrogen peroxide treatment at 4°C and 37°C in L5178Y sublines, Mutat. Res. 308 (1994) 233-241.
6. J.H. Zar, Biostatistical Analysis, Prentice-Hall, Englewood Cliffs, NJ, 1984.
7. S.J. Duthie, A. Ma, M.A. Ross, A.R. Collins, Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes, Cancer Res. 56 (1996) 1291-1295.
8. C.S. Aaron, D.M. Zimmer, P.R. Harbach, R.L. Yu, End-points for biomonitoring: assay sensitivity/selectivity, Environ. Health Perspect. 104 (1996) 521-552.
9. A.R. Collins, M. Dusinska, C.M. Gedik, R. Stetina, Oxidative damage to DNA: do we have a reliable biomarker?, Environ. Health Perspect. 104 (1996) 465-469.
10. N.P. Singh, R.R. Tice, R.E. Stephens, E.L. Schneider, A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides, Mutat. Res. 252 (1991) 289-296.
11. H. Kasai, S. Nishimura, Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents, Nucleic Acid Res. 12 (1984) 2137-2145.
12. H. Kasai, S. Nishimura, Formation of 8-hydroxydeoxyguanosine in DNA by oxygen radicals and its biological significance, in: H. Sies (Ed.), Oxidative Stress: Oxidants and Antioxidants, Academic Press, New York, 1991, pp. 99-116.
13. S. Loft, A. Astrup, B. Buemann, H.E. Poulsen, Oxidative DNA damage correlates with oxygen consumption in humans, FASEB J. 8 (1994) 534-537.
14. V.L. Wilson, B.G. Taffe, P.G. Shields, A.C. Povey, C.C. Harris, Detection and quantitation of 8-hydroxydeoxuguanosine adducts in peripheral blood of people exposed to ionizing radiation, Environ. Health Perspect. 99 (1993) 261-263.
15. T. Takeuchi, M. Nakajima, Y. Ohta, K. Mure, T. Takeshita, K. Morimoto, Evaluation of 8-hydroxydeoxuguanosine, a typical oxidative DNA damage, in human leukocytes, Carcinogenesis 15 (1994) 1519-1523.
16. A.P. Grollman, M. Moriya, Mutagenesis by 8-oxoguanine: an enemy within, Trends in Genetics 9 (1993) 246-249.