Modification of the alkaline Comet assay to allow simultaneous evaluation of mitomycin C-induced DNA cross-link damage and repair of specific DNA sequences in RT4 cells

DNA Repair

Volumn 2, Issue 8, 2003, Pages 879-890

  McKenna, Declan J ^a   Gallus, Massimo ^a   McKeown, Stephanie R ^a   Downes, C Stephen ^a   McKelvey Martin, Valerie J ^a  

^a UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

Bladder cancer; Comet FISH assay; DNA repair; Mitomycin C; TP53 gene

Indexed keywords

MITOMYCIN C;

ALKALINITY; ARTICLE; CANCER CELL CULTURE; CHEMICAL MODIFICATION; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA CROSS LINKING; DNA FLANKING REGION; DNA HYBRIDIZATION; DNA REPAIR; DNA SEQUENCE; DNA STRAND BREAKAGE; DNA TRANSFER; DOSE TIME EFFECT RELATION; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; REDUCTION KINETICS;

EID: 0142029127     PISSN: 15687864     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1568-7864(03)00086-7     Document Type: Article

References (28)

1. Collins A.R., Dobson V.L., Dusinska M., Dennedy G., Stetena R. The Comet assay: what can it really tell us? Mutat. Res. 375:1997;183-193.
2. Fairbairn D.W., Olive P.L., O'Neill K.L. The Comet assay: a comprehensive review. Mutat. Res. 339:1995;37-59.
3. Miyamae Y., Iwasake K., Kinae N., Tsuda S., Murakami M., Tanaka M., Sasaki Y.F. Detection of DNA lesions induced by chemical mutagens by the single cell electrophoresis (Comet) assay. 2. Relationship between DNA migration and alkaline condition. Mutat Res. 393:1997;107-113.
4. Olive P.L., Wlodek D., Durand R.E., Banath J.P. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res. 198:1992;259-267.
5. Merk O., Speit G. Detection of cross-links with the Comet assay in relationship to genotoxicity and cytotoxicity. Environ. Mol. Mutagen. 33:1999;167-172.
6. Pfuhler S., Wolf H.U. Detection of DNA-cross-linking agents with the alkaline Comet assay. Environ. Mol. Mutatgen. 27:1996;196-201.
7. Santos S.J., Singh N.P., Natarajan A.T. Fluorescence in situ hybridization with Comets. Exp. Cell Res. 232:1997;407-411.
8. McKelvey-Martin V.J., Ho E.T.S., McKeown S.R., Johnston S.R., McCarthy P.J., Rajab N.F., Downes C.S. Emerging applications of the single cell gel electrophoresis (Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells. Mutagenesis. 13:1998;1-8.
9. McKenna D.J., Rajab N.F., McKeown S.R., McKerr G., McKelvey-Martin V.J. Use of the Comet-FISH assay to demonstrate repair of the TP53 gene region in two human bladder carcinoma cell-lines. Radiat. Res. 159:2003;49-56.
10. Pow-Sang J.M., Seigne J.D. Contemporary management of superficial bladder cancer. Cancer Control. 7:2000;335-339.
11. Chresta C.M., Masters J.R., Hickman J.A. Hypersensitivity of human testicular tumors to etoposide-induced apoptosis is associated with functional p53 and a high Bax:Bcl-2 ratio. Cancer Res. 56:1996;1834-1841.
12. Ostling O., Johanson K.J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123:1984;291-298.
13. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., Sasaki Y.F. Single cell gel/Comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35:2000;206-221.
14. Speit G., Schutz P., Merk O. Induction and repair of formaldehyde- induced DNA-protein cross-links in repair-deficient human cell lines. Mutagenesis. 15:2000;85-90.
15. Meyn R.E., Jenkins S.F., Thompson L.H. Defective removal of DNA cross-links in a repair-deficient mutant of Chinese hamster cells. Cancer Res. 42:1982;3106-3110.
16. Plooy A.C., Dijk M., Brends F., Lohman P.H. Formation and repair of DNA interstrand cross-links in relation to cytotoxicity and unscheduled DNA synthesis induced in control and mutant human cells treated with cis-diamminedichloroplatinum(II). Cancer Res. 45:1985;4178-4184.
17. Bohr V.A., Smith C.A., Okumoto D.S., Hanawalt P.C. DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall. Cell. 40:1985;359-369.
18. Venema J., Bartosova Z., Natarajan A.T., van Zeeland A.A., Mullenders L.H. Transcription affects the rate but not the extent of repair of cyclobutane pyrimidine dimers in the human adenosine deaminase gene. J. Biol. Chem. 267:1992;8852-8856.
19. Gao S., Drouin R., Holmquist G.P. DNA repair rates mapped along the human PGKI gene at nucleotide resolution. Science. 263:1994;1438-1440.
20. Bock C., Rapp A., Dittmar H., Monajembashi S., Greulich K.-O. Localization of specific sequences and DNA single strand breaks in individual UV-A irradiated human lymphocytes by Comet-FISH. Prog. Biochem. Opt. SPIE. 3568:1999;207-217.
21. Menke M., Angelis K.J., Schubert I. Detection of specific DNA lesions by a combination of Comet assay and FISH in plants. Environ. Mol. Mutagen. 35:2000;132-138.
22. Dean C.T., Ormerod M.G., Seriani R.W., Alexander P. DNA strand breakings in cells irradiated with X-rays. Nature. 222:1969;1042-1044.
23. G. Ahnström, K. Erixon, Measurement of strand breaks by alkaline denaturation and hydroxylapatite chromatography, in: E.C. Friedberg, P.C. Hanawalt (Eds.), DNA Repair, vol. 1 (Part A), Marcel Dekker, New York, 1981, pp. 403-418.
24. McGlynn A.P., Wasson G., O'Connor J., McKerr G., McKelvey-Martin V.J., Downes C.S. The bromodeoxyuridine Comet assay: detection of maturation of recently replicated DNA in individual cells. Cancer Res. 59:1999;5912-5916.
25. Evans M.K., Taffe B.G., Harris C.C., Bohr V.A. DNA strand bias in the repair of the p53 gene in normal human and xeroderma pigmentosum group C fibroblasts. Cancer Res. 53:1993;5377-5381.
26. Ford J.M., Lommel L., Hanawalt P.C. Preferential repair of ultraviolet light-induced DNA damage in the transcribed strand of the human p53 gene. Mol. Carcinogen. 10:1994;105-109.
27. Larminat F., Zhen W., Bohr V.A. Gene-specific DNA repair of interstrand cross-links induced by chemotherapeutic agents can be preferential. J. Biol. Chem. 268:1993;2649-2654.
28. Meniel V., Magana-Schwencke N., Averback D. Preferential repair in yeast after induction of interstrand cross-links by 8-methoxypsoralen plus UVA. Mutat. Res. 329:1995;121-130.