Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Ukkonen, Kaisa ^a,b   Veijola, Johanna ^a   Vasala, Antti ^b   Neubauer, Peter ^c  

^a UNIVERSITY OF OULU   (Finland)

^b BioSilta Oy   (Finland)

^c TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Autoinduction; Fab fragment; Fed batch; Oxygen transfer; Periplasmic expression

Indexed keywords

GLYCEROL; IMMUNOGLOBULIN F(AB) FRAGMENT; OXYGEN;

AERATION; ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; FED BATCH CULTURE; GLUCOSE INTAKE; NONHUMAN; OXYGEN TRANSPORT; PRODUCT RECOVERY; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SPECIES CULTIVATION;

BATCH CELL CULTURE TECHNIQUES; CULTURE MEDIA; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FAB FRAGMENTS; OXYGEN; PERIPLASM; RECOMBINANT PROTEINS;

ESCHERICHIA COLI K12;

EID: 84880886319     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-73     Document Type: Article

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